Construction of three quadruple-fluorescent MDA435 cell lines that enable monitoring of the whole chromosome segregation process in the living state

Mechanism of induction of binucleated cells by multiwalled carbon nanotubes as revealed by live-cell imaging analysis

“…Dichromatically visualized MDA-435 human breast cancer cells, in which chromosomes and centromeres were stained with a red fluorescent protein (mCherry–Histone H3 fusion) and green fluorescent protein (EGFP–CENP-A fusion), respectively, were kindly provided by Dr. Kenji Sugimoto (Osaka Prefecture University, Osaka, Japan) [ 20 ]. The cells were cultured at 37 °C (5 % CO 2 , 100 % humidity) in Dulbecco’s Modified Eagle’s medium (DMEM) (Nacalai Tesque, Kyoto, Japan), supplemented with 10 % fetal bovine serum.…”

Visualization of Aberrant Perinuclear Microtubule Aster Organization by Microtubule-Destabilizing Agents

“…pmCherry--tubulin, pmKO-AF8, 10) and pECFPhistone H3 7) were separately introduced into MDA-GFP-Aurora-A cells 7) with selection plasmids, pTKHyg, pPur, and pCAGGS-bsr respectively. Stable transformants were sequentially selected by hygromycin B, puromycin, and blasticidin to establish MDA AuroA/ tub/AF8/H3 (clone 7-1).…”

Synthesis of mouse centromere-targeted polyamides and physico-chemical studies of their interaction with the target double-stranded DNA

“…[14][15][16] In addition, they do not modify cell genomes as in case of artificial engineered constructs that express different DNA-binding peptides or proteins fused with colored fluorescent proteins (Green Fluorescent Protein, GFP, and others). [17][18][19][20][21][22][23][24][25][26][27] Fluorescent polyamide tandems with enhanced specificity were used to label telomeric and centromeric repeats in chromosomes, 28 fixed insect and mammalian cells. [29][30][31] Cells were permeabilized in order to facilitate probe access to DNA and to remove the probe excess for eliminating a high fluorescent background.…”