Construction of tissue-engineered nucleus pulposus by stimulation with periodic mechanical stress and BMP-2

Liu, Yang; Gao, Guoquan; Yang, K.G. Auw; Nong, Luming

doi:10.1016/j.isci.2022.104405

Cited by 5 publications

(9 citation statements)

References 68 publications

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“…We provide a novel interaction network of circRNA/miRNA/mRNA in IVDD progression. Based on the competing endogenous RNA mechanism, circFGFBP1 can modulate BMP2 to suppress the degenerative histopathological changes in the intervertebral disc and induce the regeneration of NP cells [ 24 , 25 , 26 ]. Moreover, we also determined the new role of miR-9-5p in NP cells during IVDD.…”

Section: Discussionmentioning

confidence: 99%

FOXO3-Activated circFGFBP1 Inhibits Extracellular Matrix Degradation and Nucleus Pulposus Cell Death via miR-9-5p/BMP2 Axis in Intervertebral Disc Degeneration In Vivo and In Vitro

et al. 2023

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(1) Background: intervertebral disc degeneration (IVDD) defined as the degenerative changes in intervertebral disc is characterized by extracellular matrix (ECM) degradation and death in nucleus pulposus (NP) cells. (2) Methods: The model of IVDD was established in male Sprague Dawley rats using a puncture of a 21-gauge needle at the endplates located in the L4/5 intervertebral disc. Primary NP cells were stimulated by 10 ng/mL IL-1β for 24 h to mimic IVDD impairment in vitro. (3) Results: circFGFBP1 was downregulated in the IVDD samples. circFGFBP1 upregulation inhibited apoptosis and extracellular matrix (ECM) degradation and promoted proliferation in IL-1β-stimulated NP cells. Additionally, circFGFBP1 upregulation mitigated the loss of NP tissue and the destruction of the intervertebral disc structure in vivo during IVDD. FOXO3 could bind to the circFGFBP1 promoter to enhance its expression. circFGFBP1 upregulated BMP2 expression in NP via sponging miR-9-5p. FOXO3 enhanced the protection of circFGFBP1 in IL-1β-stimulated NP cells, whereas a miR-9-5p increase partly reversed the protection. miR-9-5p downregulation contributed to the survival of IL-1β-stimulated NP cells, which was partially reversed by BMP2 silence. (4) Conclusions: FOXO3 could activate the transcription of circFGFBP1 via binding to its promoter, which resulted in the enhancement of BMP2 via sponging miR-9-5p and then inhibited apoptosis and ECM degradation in NP cells during IVDD.

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Section: Discussionmentioning

confidence: 99%

FOXO3-Activated circFGFBP1 Inhibits Extracellular Matrix Degradation and Nucleus Pulposus Cell Death via miR-9-5p/BMP2 Axis in Intervertebral Disc Degeneration In Vivo and In Vitro

et al. 2023

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“…The large changes in pressure between the degenerative IDs leads to a decrease in the survival and differentiation of MSCs[ 56 ]. Medullary pulposus cell-derived hydrogels help MSCs differentiate into nucleus pulposus cells[ 57 ].…”

Section: Harsh Environment Of the Idmentioning

confidence: 99%

How to enhance the ability of mesenchymal stem cells to alleviate intervertebral disc degeneration

Zhang,

Cui

2023

World J Stem Cells

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Intervertebral disc (ID) degeneration (IDD) is one of the main causes of chronic low back pain, and degenerative lesions are usually caused by an imbalance between catabolic and anabolic processes in the ID. The environment in which the ID is located is harsh, with almost no vascular distribution within the disc, and the nutrient supply relies mainly on the diffusion of oxygen and nutrients from the blood vessels located under the endplate. The stability of its internal environment also plays an important role in preventing IDD. The main feature of disc degeneration is a decrease in the number of cells. Mesenchymal stem cells have been used in the treatment of disc lesions due to their ability to differentiate into nucleus pulposus cells in a nonspecific anti-inflammatory manner. The main purpose is to promote their regeneration. The current aim of stem cell therapy is to replace the aged and metamorphosed cells in the ID and to increase the content of the extracellular matrix. The treatment of disc degeneration with stem cells has achieved good efficacy, and the current challenge is how to improve this efficacy. Here, we reviewed current treatments for disc degeneration and summarize studies on stem cell vesicles, enhancement of therapeutic effects when stem cells are mixed with related substances, and improvements in the efficacy of stem cell therapy by adjuvants under adverse conditions. We reviewed the new approaches and ideas for stem cell treatment of disc degeneration in order to contribute to the development of new therapeutic approaches to meet current challenges.

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“…18,19 To date, biomaterials for the treatment of degenerated NP are either synthetically manufactured permanent acellular implants 20 or degradable materials which rely on cell growth and proliferation to form neo-NP tissue at the site of degeneration to illicit repair. 21 In all NP replacement strategies, the mechanical properties of biomaterials are of great significance. Simple uniaxial compression tests are far from adequate when considering the six degrees of freedom motions (flexion, extension, right and left lateral bending, compression, and axial rotation) experienced in the human spine.…”

Section: Introductionmentioning

confidence: 99%

“…To date, biomaterials for the treatment of degenerated NP are either synthetically manufactured permanent acellular implants 20 or degradable materials which rely on cell growth and proliferation to form neo‐NP tissue at the site of degeneration to illicit repair 21 . In all NP replacement strategies, the mechanical properties of biomaterials are of great significance.…”

Section: Introductionmentioning

confidence: 99%

Mechanical characterization and design of biomaterials for nucleus pulposus replacement and regeneration

Honiball

et al. 2023

J Biomedical Materials Res

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Biomaterials for nucleus pulposus (NP) replacement and regeneration have great potential to restore normal biomechanics in degenerated intervertebral discs following nucleotomy. Mechanical characterizations are essential for assessing the efficacy of biomaterial implants for clinical applications. While traditional compression tests are crucial to quantify various modulus values, relaxation behaviors and fatigue resistance, rheological measurements should also be conducted to investigate the viscoelastic properties, injectability, and overall stability upon deformation. To recapitulate the physiological in vivo environment, the use of spinal models is necessary to evaluate the risk of implant extrusion and the restoration of biomechanics under different loading conditions. When designing devices for NP replacement, injectable materials are ideal to fully fill the nucleus cavity and prevent implant migration. In addition to achieving biocompatibility and desirable mechanical characteristics, biomaterial implants should be optimized to avoid implant extrusion or re‐herniation post‐operatively. This review discusses the most commonly used testing protocols for assessing mechanical properties of biomaterial implants and serves as reference material for enabling researchers to characterize NP implants through a unified approach whereby newly developed biomaterials may be compared and contrasted to existing devices.

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Construction of tissue-engineered nucleus pulposus by stimulation with periodic mechanical stress and BMP-2

Cited by 5 publications

References 68 publications

FOXO3-Activated circFGFBP1 Inhibits Extracellular Matrix Degradation and Nucleus Pulposus Cell Death via miR-9-5p/BMP2 Axis in Intervertebral Disc Degeneration In Vivo and In Vitro

FOXO3-Activated circFGFBP1 Inhibits Extracellular Matrix Degradation and Nucleus Pulposus Cell Death via miR-9-5p/BMP2 Axis in Intervertebral Disc Degeneration In Vivo and In Vitro

How to enhance the ability of mesenchymal stem cells to alleviate intervertebral disc degeneration

Mechanical characterization and design of biomaterials for nucleus pulposus replacement and regeneration

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