Construction of tissue-engineered osteochondral composites and repair of large joint defects in rabbit

Deng, Tianzheng; Lv, Jing; Pang, Jianliang; Liu, Bing; Ke, Jie

doi:10.1002/term.1556

Cited by 27 publications

(29 citation statements)

References 60 publications

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“…Fifteen studies [49, 65, 72, 75, 81, 86, 89–97] used a mixture of solutions prepared from hyaluronic acid [65, 92, 94–97], phosphate buffer solution [91], plasma [75], basal medium with chondrogenesis [89], collagen acid [93], sodium alginate [86] or a growth factor medium [90]. Two studies used MSCs only [49, 72].…”

Section: Resultsmentioning

confidence: 99%

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

et al. 2017

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BackgroundThe management of articular cartilage defects presents many clinical challenges due to its avascular, aneural and alymphatic nature. Bone marrow stimulation techniques, such as microfracture, are the most frequently used method in clinical practice however the resulting mixed fibrocartilage tissue which is inferior to native hyaline cartilage. Other methods have shown promise but are far from perfect. There is an unmet need and growing interest in regenerative medicine and tissue engineering to improve the outcome for patients requiring cartilage repair. Many published reviews on cartilage repair only list human clinical trials, underestimating the wealth of basic sciences and animal studies that are precursors to future research. We therefore set out to perform a systematic review of the literature to assess the translation of stem cell therapy to explore what research had been carried out at each of the stages of translation from bench-top (in vitro), animal (pre-clinical) and human studies (clinical) and assemble an evidence-based cascade for the responsible introduction of stem cell therapy for cartilage defects.Main body of abstractThis review was conducted in accordance to PRISMA guidelines using CINHAL, MEDLINE, EMBASE, Scopus and Web of Knowledge databases from 1st January 1900 to 30th June 2015. In total, there were 2880 studies identified of which 252 studies were included for analysis (100 articles for in vitro studies, 111 studies for animal studies; and 31 studies for human studies). There was a huge variance in cell source in pre-clinical studies both of terms of animal used, location of harvest (fat, marrow, blood or synovium) and allogeneicity. The use of scaffolds, growth factors, number of cell passages and number of cells used was hugely heterogeneous.Short conclusionsThis review offers a comprehensive assessment of the evidence behind the translation of basic science to the clinical practice of cartilage repair. It has revealed a lack of connectivity between the in vitro, pre-clinical and human data and a patchwork quilt of synergistic evidence. Drivers for progress in this space are largely driven by patient demand, surgeon inquisition and a regulatory framework that is learning at the same pace as new developments take place.

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Section: Resultsmentioning

confidence: 99%

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

et al. 2017

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“…Cell sources and biological protein incorporation methods are discussed, addressing their interaction with scaffolds and highlighting the potential for creating a new generation of bilayered composite scaffolds that can mimic the native interfacial tissue properties, and are able to adapt to the biological environment 39 . Deng et al 45 evaluated the efficacy of a three-dimensional (3D) heterogeneous/bilayered scaffold, consists of gelatin, chondroitin sulphate and sodium hyaluronate (GCH) and ceramic bovine bone (GCBB), to repair large defects in rabbit joints. In summary, this study demonstrated that a novel scaffold, comprising a top layer of GCH, having mechanical properties comparable to native cartilage, and combined with chondrocytes and bone marrow stem cells (BMSCs) could be used to repair large osteochondral defects in joints.…”

Section: Resultsmentioning

confidence: 99%

The use of PLDLA/PCL-T scaffold to repair osteochondral defects in vivo

et al. 2012

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The physiological repair of osteochondral lesions requires the development of a scaffold that is compatible with the structure of the damaged tissue, cartilage and bone. The aim of this study was to evaluate the biological performance of a PLDLA/PCL-T (90/10) scaffold for repairing osteochondral defects in rabbits. Polymeric scaffolds containing saccharose (75% w/v) were obtained by solvent casting and then implanted in the medial knee condyles of 12 New Zealand rabbits after osteochondral damage with a trephine metallic drill (diameter: 3.3 mm) in both medial femoral condyles. Each rabbit received the same treatment, i.e., the polymeric scaffold was implanted on the right side while no material was implanted on the left side (control). Four and 12 weeks later histological examination revealed bone neoformation in the implant group, with the presence of hyaline cartilage and mesenchymal tissue. In contrast, the control group showed bone neoformation with necrosis, exacerbated superficial fibrosis, inflammation and cracks in the neoformed tissue. These findings indicate that the PLDLA/PCL-T scaffold was biocompatible and protected the condyles by stabilizing the lesion and allowing subchondral bone tissue and hyaline cartilage formation.

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“…high substrate stiffness and normoxic oxygen tension. The substrate stiffness was determined based on the phenotype of the cells in the surrounding lattice points 3 . The stiffness was considered high if the differentiating MSC was within the vicinity of a mature OB.…”

Section: Discussionmentioning

confidence: 99%

“…The goal of these strategies is to use cells, scaffolds and growth factors to regenerate the damaged tissue and thus provide long term relief to the patient (2). While numerous advances have been made in the design of multiphasic scaffolds (3)(4)(5), cell culture protocols (6,7) and growth factor releasing materials (8,9), a number of issues still exist which limit the widespread implementation of these techniques. One of the most prominent of these is promoting the formation of stable bone and cartilage within the osseous and chondral regions of the defect respectively.…”

Section: Introductionmentioning

confidence: 99%

A Computational Model of Osteochondral Defect Repair Following Implantation of Stem Cell-Laden Multiphase Scaffolds

O'Reilly

Kelly

2017

Tissue Engineering Part A

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Tissue engineering (TE) has recently emerged as a potential method with which to treat osteochondral defects. In order to improve these strategies, an understanding is required of how the cells within an implanted scaffold interact with their local environment.To this end, the objective of this study was to develop a computational model of an osteochondral defect following the implantation of a tissue engineered scaffold. In particular, the purpose of this was to systematically explore the relationship that scaffold design has on the spatial formation of cartilage and bone within the defect. In this study, tissue formation was predicted using a previously developed algorithm in which cell fate was dependent upon the local oxygen tension and the local mechanical environment. In the first part of this study, the model was updated to include a condition whereby mature cartilage was resistant to both terminal differentiation and vascularisation. The updated model was then tested by using it to predict tissue formation during the spontaneous repair process within an osteochondral defect. Following this, the model was used to simulate an osteochondral defect treated with different variations of a previously developed multiphasic scaffold. By comparing the results of the different simulations it was possible to elucidate a mechanism by which the scaffold promoted robust bone and cartilage formation. In particular, support was provided for a previous hypothesis which proposed that osteochondral defect repair can be enhanced by actively confining angiogenesis to within the subchondral region of the defect for a sustained period of time. Based on the results of this study, it was possible to expand on this by suggesting that this time period be no less than 10 weeks. It is possible to reduce this, however, by pre-culturing MSCs under chondrogenic conditions prior to implantation within the defect.

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Construction of tissue-engineered osteochondral composites and repair of large joint defects in rabbit

Cited by 27 publications

References 60 publications

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

The use of mesenchymal stem cells for cartilage repair and regeneration: a systematic review

The use of PLDLA/PCL-T scaffold to repair osteochondral defects in vivo

A Computational Model of Osteochondral Defect Repair Following Implantation of Stem Cell-Laden Multiphase Scaffolds

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