Construction of two Listeria ivanovii attenuated strains expressing Mycobacterium tuberculosis antigens for TB vaccine purposes

Lin, Qingqing; Zhou, Ming; Xu, Zongkai; Khanniche, Asma; Shen, Hao; Wang, Chuan

doi:10.1016/j.jbiotec.2015.01.008

Cited by 13 publications

(28 citation statements)

References 24 publications

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“…The immune responses induced by intranasal administration of the two recombinant strains were quite different from those induced by intravenous administration. After intravenous administration, bacteria were delivered via circulation of the blood and directly entered peripheral immune organs such as the spleen and liver, where they were phagocytosed by phagocytes and dendritic cells, thus inducing efficient systemic immune responses 17 . Our earlier studies showed that wild-type LI mainly localized in the lung after intranasal administration 18 .…”

Section: Discussionmentioning

confidence: 99%

“…Western blot analysis. Proteins from LIΔ-Rv3875 and LIΔ-Rv0129c were obtained by trichloroacetic acid precipitation as described previously 17 . Proteins were resolved by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes.…”

Section: Discussionmentioning

confidence: 99%

“…The lungs were removed and digested with 1 mg/ml collagenase I (Sigma, USA) and 0.1 mg/ml DNase I (Sigma, USA) to harvest lung cell suspensions for cytokine detection. Splenocyte suspensions for cytokine detection were prepared according to a previous study 17 . BAL was collected by making a small incision in the trachea and injecting 1 ml pre-cold phosphate buffer solution (PBS) with an indwelling needle.…”

Section: Discussionmentioning

confidence: 99%

“…Investigating the ability of recombinant LI strains integrated with heterologous antigens to induce immune responses via intranasal vaccination is valuable in evaluating LI as a good antigen delivery vehicle for vaccines against respiratory tract infections. In this study, we evaluated the biosafety and immunogenicity of intranasal vaccination using two recombinant LI strains, LIΔactAplcB-Rv3875 (LIΔ-Rv3875) and LIΔactAplcB-Rv0129c (LIΔ-Rv0129c) 17 , to verify our hypothesis. We found that both strains induced lung-localized immune responses, including antigen-specific cellular immune responses and the secretion of secretory IgA (SIgA).…”

mentioning

confidence: 90%

“…Hence, LI is a safer live vaccine vehicle compared to that of LM. Recently, two recombinant attenuated LI strains expressing the ESAT-6 or Ag85C protein of Mtb were constructed, and an antigen-specific CD8 + T cell-mediated immune response was obtained via intravenous vaccination 17 . However, whether such recombinant strains could induce lung-localized and systemic cellular and humoral immune responses when intranasally administered to mice is still unknown.…”

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confidence: 99%

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Intranasal vaccination with Listeria ivanovii as vector of Mycobacterium tuberculosis antigens promotes specific lung-localized cellular and humoral immune responses

Jiang

Liu

et al. 2020

Sci Rep

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We have previously demonstrated that a recombinant Listeria ivanovii (LI) strain expressing the ESAT-6 or Ag85C protein of Mycobacterium tuberculosis (Mtb) as a tuberculosis (TB) vaccine candidates induced antigen-specific cellular immune responses after intravenous immunization of mice. However, whether such recombinant strains could induce desired immune responses in the lung, where TB infection occurs, is not clear. In this paper, C57BL/6 J mice were intranasally vaccinated with attenuated LIΔactAplcB-Rv3875 (Δ refers to gene deletion in the bacterial genome) or LIΔactAplcB-Rv0129c, the two vaccine candidates that utilize LI as an antigen delivery vector. Bacterial load in the target organs, histological changes in the infected organs, the percentage of specific cytokine-secreting T cells in the lung and spleen, IgG levels in the serum and secretory IgA (SIgA) levles in bronchoalveolar lavage (BAL) fluid were determined at specific days post inoculation (dpi). The results showed that both strains were mainly confined to the lung and were eliminated at 10 dpi. The histological damage caused by the infection in the lung was slight and recovered by day 5. Intranasal vaccination of the mice twice at an interval of 4 weeks notably elicited TB antigen-specific CD4 + and CD8 + T cell responses in the lung and SIgA secretion in the pulmonary mucosa, and significantly enhanced the percentage of double-functional CD8 + t cells (IFN-γ + TNF-α + CD8 + ). To our knowledge, this is the first report regarding the used of LI vector vaccines to induce promising lung-localized cellular and humoral immune responses by intranasal vaccination. These data suggest that LI could be a novel and promising live vector to construct an intranasal vaccine against respiratory diseases.Mice. Six-to eight-week-old female C57BL/6 J mice were purchased from the Animal Centre of Sichuan Province Hospital (Chengdu, China). All mice were maintained under specific pathogen-free conditions throughout the experiments at the Animal Centre of the School of Public Health at Sichuan University. Mouse experiments were performed according to the guidelines of the Animal Care and Use Committee of Sichuan University, and the protocols for mouse experiments were approved by this committee as well.Strains. LIΔ-Rv3875 and LIΔ-Rv0129c, expressing Mtb ESAT-6 or Ag85C protein respectively were constructed as described in a previous study 17 . LIΔ was constructed and maintained in our laboratory as a vector control 17 . All strains were cultured at 37 °C with shaking at 180 r/m in brain heart infusion (BHI) broth. Scientific RepoRtS |(2020) 10:302 | https://doi.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 90%

mentioning

confidence: 99%

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