Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation

Abstract

Sensitive precise measurements were taken (high signal-to-noise ratios, 10-Pm image 24 resolution, 99% scan-to-scan reproducibility). These features confirmed their potential as 25 analytical tools, are a competitive alternative to fluorescence scanners, and incorporate additional advantages, such as user-friendly interface and connectivity for telemedicine needs.The analytical performances of the integrated platform (assay and reader) in the human samples were also excellent, with a low detection limit (100 genomic DNA copies), and reproducible (<15%) and cheap assays (< 10 €/test). The correct genotyping of a genetic biomarker (singlenucleotide polymorphism located in the GRIK4 gene) was achieved as the assigned genotypes agreed with those determined by using sequencing. The portability, favourable discriminating and read-out capabilities reveal that the implementation of mass-produced low-cost devices into minimal-specialised clinical laboratories is closer to becoming a reality.

Electrosprayed mucoadhesive alginate-chitosan microcapsules for gastrointestinal delivery of probiotics

“…Accordingly, the mucoadhesive properties of unloaded alginate, alginate-starch and chitosan-coated alginate microparticles were evaluated using an in vitro fluorescence imagingbased flow-through test on ex vivo porcine gastric epithelial mucosa, following the protocol described previously by the Khutoryanskiy group (Cook et al, 2018;Kaldybekov et al, 2018;Porfiryeva et al, 2019). As some recent reports also suggested the potential utilisation of a lowcost commercial USB microscope in different imaging-related assays (Bracker and Stender, 2019;Tortajada-genaro et al, 2019), here, we attempted to adapt this device for imaging the samples in the present mucoadhesion study, with assessing the usability of that as an alternative imaging tool for this study.…”

Fast DNA biosensing based on isothermal amplification, unmodified gold nanoparticles, and smartphone detection

“…Compared to other isothermal processes, this reaction shows essential advantages, such as low working temperature (36-40 • C), simple primer design, fast-amplification (20-60 min), no initial heating step, and robust to biological substances (Lobato & O'Sullivan, 2018). In addition, this amplification is compatible with several assay formats for point-of-need applications (Santiago-Felipe et al, 2016;Tortajada-Genaro et al, 2019). The goal was to generate RPA products with single-strand regions to modify the color of gold colloidal suspension, which can be detected with the naked eye even at a relatively low concentration.…”

A review of smartphone point‐of‐care adapter design

“…13 Recent innovations verified that smartphone-based applications are very promising demonstrating precise measurements, [14][15][16][17][18] a low limit of detection (LOD), [19][20][21][22][23] a wide dynamic range, [24][25][26][27] low power consumption, easy-to-use smart applications, a low coefficient of variation (CV%), a high regression coefficient (R 2 ), high specificity, reliable sensitivity, rapid testing, cost-effective adapter designs, and high selectivity. [28][29][30][31][32] These innovations include the major parts of POC applications, such as surgical treatment application (Section 3.2), surgical diagnosis (Section 3.1), ophthalmic applications (Section 3.3), biochemical applications (Section 3.4), [33][34][35] environmental monitoring applications, [36][37][38][39][40][41][42] biomedical applications, [43][44][45] electrochemical applications (Section 3.5), colorimetric applications, [46][47][48][49] imaging applications, [50][51][52][53][54] and spectrometry applications. …”