The production of microalgal biomass and products derived thereof for a wide variety of applications is a hot research topic, with the number of facilities being built and products and biologically active molecules launched into the market increasing every year. The aim of the current study was to identify the attitudes of citizens in Almería (Spain) and Livorno (Italy) towards the construction of a microalgae production plant and a biorefinery in their cities and also their opinions about the microalgae-based products that could be produced. Overall, in Almería (Spain), a NIMBY (not in my back yard) attitude towards the construction of a microalgal production facility and especially towards a microalgal biorefinery was observed, despite the strong microalgal industry in the region and the higher knowledge of citizens about microalgae. In both locations, but especially in Livorno (Italy), microalgae-based biostimulants, biofertilisers, and aquafeeds were well accepted. Proximity was the main factor affecting the acceptance of a microalgae producing facility. Consumer knowledge about microalgal biotechnology and the health and environmental benefits of this valuable raw material are scarce, and opinions are based on drivers other than knowledge. After gaining more knowledge about microalgal biorefineries, most of the responses in Almería (47%) and Livorno (61%) were more positive.