Abstract-In order to allow consumers to trace to the whole process of beef breeding, production and processing after purchasing beef, to achieve smart, fast and practical goals for quality and safety of beef traceability system, B / S (Browser / Server) mode architecture is used to build quality beef traceability system based on RFID technology in this paper. First, the system is analyzed, the structure and database system are designed and the key technologies to achieve traceability system is explored, including the individual identification of cattle, data encoding, data synchronization technology between the business and the central database and multi-platform tracing techniques.Through the use of RFID technology, all aspects of information for each head of beef cattle from breeding, slaughtering, processing, cold chain storage and transportation can be tracked and traced, and a quality retrospective file which can be queried on the Internet network for each head of beef cattle is formed. Solve the problem which the origin of beef production can be quality controlled and is traceability. The system implements safety warning function for cattle breeding epidemic and product quality, so it can ensure the safety of beef products from all aspects of production management. Finally, the renderings of system implementation are gained.