Consumption of cruciferous vegetables and glucosinolates in a Spanish adult population

Agudo, Antonio; Ibáñéz, Raquel Sanchez; Amiano, Pilar; Ardanaz, Eva; Barricarte, Aurelio; Berenguer, Antonio; Chirlaque, María Dolores; Dorronsoro, Miren; Jakszyn, Paula; Larrañaga, Nerea; Martínez, Carmen; Navarro, Carmen; Pera, Guillem; Quiŕos, J. Ramón; Sánchez, María José; González, Carlos

doi:10.1038/sj.ejcn.1602750

Cited by 32 publications

(22 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…described the intake of cruciferous vegetables in their populations as servings per week, and other cohort studies used fewer vegetables to compute their GLS‐containing food consumption data than we did. For the Spanish male EPIC cohort, a median intake of cruciferous vegetables of 4.1 g/day was reported based on data obtained by diet history method,32 which is considerably less than the median intake in our study (15.8 g/day). However, this may be largely explained by a higher consumption of cabbages in Germany because of national consumption preferences 33.…”

Section: Discussioncontrasting

confidence: 74%

Dietary glucosinolate intake and risk of prostate cancer in the EPIC‐Heidelberg cohort study

Steinbrecher

Nimptsch

Hüsing

et al. 2009

Intl Journal of Cancer

View full text Add to dashboard Cite

Glucosinolates (GLS) are secondary plant metabolites occurring in cruciferous vegetables. Their biologically active break-down products show cancer preventive properties in animal and cell studies. So far, epidemiologic studies, using consumption of cruciferous vegetables as proxy for GLS intake, yielded inconsistent results. Here, we evaluated the association between dietary intake of GLS in comparison with consumption data of GLS-containing foods and the risk of prostate cancer. The study population comprised 11,405 male participants of the prospective EPIC-Heidelberg cohort study. During a mean follow-up time of 9.4 years, 328 incident cases of prostate cancer occurred. At recruitment, habitual food consumption was assessed by a validated food frequency questionnaire, and intake of individual GLS was estimated by means of a newly compiled database on food content of GLS. Adjusted hazard ratios (HR) for prostate cancer were calculated using the Cox proportional hazard model. Median daily intake of total GLS was 7.9 mg/day (interquartile range 5.1-11.9 mg/day). The risk of prostate cancer decreased significantly over quartiles of total GLS intake (multivariate HR [4th vs. 1st quartile] 0.68, 95% CI 0.48-0.97, p trend 0.03). Associations with GLS-containing food intake were weaker. Among GLS subgroups, aliphatic GLS showed the strongest inverse association with cancer risk. Analyses stratified by tumor stage and grade gave hint to inverse associations for localized and low-grade cancers. This study shows an inverse association between dietary intake of GLS and the risk of prostate cancer. Because this is the first prospective study using individual GLS intake data, confirmation in other studies is warranted. ' UICCKey words: glucosinolates; cruciferous vegetables; prostate cancer; EPIC-Heidelberg; dietary intake Glucosinolates (GLS) are plant metabolites occurring predominantly in cruciferous vegetables.1 According to the chemical structure of their side chain 3 main groups can be distinguished: aliphatic (with alkyl or alkenyl group), aromatic (with benzyl group) and heterocyclic (with indolyl group) GLS. On cell rupture, GLS are cleaved by the plant enzyme myrosinase, and depending on the structure and reaction conditions, various biologically active components can be formed.2 Isothiocyanates-derived from aliphatic and aromatic GLS (at neutral pH)-and indoles-derived from indolyl-GLS-are currently the break-down products of most scientific interest because of their cancer preventive properties observed in cell and animal studies. Different mechanisms of anticarcinogenic activity are proposed for these break-down products such as the induction of antioxidant and detoxification genes. Furthermore, the inhibition of proinflammatory reactions, cytochrome P450 enzyme activity and histone deacetylase are under discussion as well as the activation of cell cycle arrest and apoptosis. 4 In prostate cancer cells lines it was shown, that sulforaphane, the hydrolysis product of glucoraphanine as the major aliphatic GLS ...

show abstract

Section: Discussioncontrasting

confidence: 74%

Dietary glucosinolate intake and risk of prostate cancer in the EPIC‐Heidelberg cohort study

Steinbrecher

Nimptsch

Hüsing

et al. 2009

Intl Journal of Cancer

View full text Add to dashboard Cite

show abstract

“…Furthermore, dietary habits differ not only between countries but might also vary according to regional habits or time within the same country. Additionally, some population characteristics like the age structure, educational attainment or smoking status impact on food consumption and therefore on glucosinolate intake, as shown in the present study or in the Spanish EPIC cohort [21] . These effects may be more pronounced if studies are small and not population based.…”

Section: Discussionmentioning

confidence: 78%

“…In comparison, in the Spanish EPIC cohort a mean intake of total glucosinolates of 6.8 mg/day for men and 6.2 mg/day for women was found [21] . These intake estimates were computed based on dietary data obtained via a diet history method (assessing habitual food intake) and linked with the database on total glucosinolate content of food compiled by McNaughton and Marks [5] .…”

Section: Discussionmentioning

confidence: 99%

Dietary Intake of Individual Glucosinolates in Participants of the EPIC-Heidelberg Cohort Study

Steinbrecher

Linseisen

2009

Ann Nutr Metab

View full text Add to dashboard Cite

Background/Aims: To compile a database on content of individual glucosinolates in food and to describe the dietary intake of individual glucosinolates in a German population. Methods: Studies analysing the content of individual glucosinolates in food were aggregated to form a database of 26 individual glucosinolates in 18 vegetables and condiments consumed in Germany. This database was linked to food intake data derived from 24-hour diet recalls of 2,121 participants of the EPIC-Heidelberg cohort study. Results: Mean total glucosinolate intake (±standard error) was 14.2 (±1.1) mg/day for men and 14.8 (±1.3) mg/day for women. The intake increased with age and education; smokers ingested less glucosinolates than never or former smokers. The quantitatively most important individual glucosinolates were glucobrassicin and sinigrin with mean daily intakes of 3.5 (±0.3) and 1.7 (±0.2) mg/day for men, and 4.2 (±0.4) and 2.5 (±0.4) mg/day for women, respectively. Broccoli, Brussels sprouts and cauliflower contributed most to the total glucosinolate intake in this population. Conclusions: The established database allowed for the first time the estimation of dietary intake of individual glucosinolates. The database can be used for epidemiological research on the role of glucosinolates in health and disease.

show abstract

“…Moreover, ESP has varied substrate specificity with a high efficiency on the hydrolysis of aliphatic glucosinolates compared to a small impact on aromatic glucosinolates (Cole, 1978; Kaoulla, et al, 1980; Matusheski, et al, 2004; Matusheski, et al, 2006; Petroski & Tookey, 1982; Wittstock & Burow, 2007). Therefore, although glucosinolate content in cruciferous vegetables has been reported in several studies (Agudo, et al, 2008; Kushad, et al, 1999; McNaughton & Marks, 2003; Verkerk, et al, 2009), estimation of total dietary glucosinolate intake does not represent total dietary isothiocyanate exposure in humans.…”

Section: Introductionmentioning

confidence: 98%

Total isothiocyanate yield from raw cruciferous vegetables commonly consumed in the United States

Tang

Paonessa

Zhang

et al. 2013

Journal of Functional Foods

View full text Add to dashboard Cite

Dietary isothiocyanates are a group of promising chemopreventive agents obtained primarily from cruciferous vegetables. Due to their potent chemopreventive and/or anti-cancer activities, there is a growing interest in assessing dietary isothiocyanate exposure and its impact on human health. Using the HPLC-based cyclocondensation assay, the current study measured total isothiocyanate yield from raw cruciferous vegetables. A total of 73 samples comprising nine types of cruciferous vegetables were analyzed. We observed a wide range of isothiocyanate content across the individual vegetables with an average level of 16.2 μmol/100g wet weight, ranging from 1.5 μmol in raw cauliflower to 61.3 μmol in raw mustard greens. The data represent the maximum amount of isothiocyanates released from the intake of raw cruciferous vegetables. Given that the vegetables assayed in this study include the most commonly consumed cruciferous vegetables in western diets, the data may be particularly useful in estimation of dietary isothiocyanate exposure in these populations. However, due to the variation observed within each vegetable, biomarkers such as urinary isothiocyanate level may be necessary for accurate estimation of individual exposure.

show abstract

Consumption of cruciferous vegetables and glucosinolates in a Spanish adult population

Cited by 32 publications

References 30 publications

Dietary glucosinolate intake and risk of prostate cancer in the EPIC‐Heidelberg cohort study

Dietary glucosinolate intake and risk of prostate cancer in the EPIC‐Heidelberg cohort study

Dietary Intake of Individual Glucosinolates in Participants of the EPIC-Heidelberg Cohort Study

Total isothiocyanate yield from raw cruciferous vegetables commonly consumed in the United States

Contact Info

Product

Resources

About