Consumption of H₂S from Our Daily Diet: Determination by a Simple Chemosensing Method

Abstract

A unique method has been developed for comparative analysis of H2S produced from food samples from our daily diet, both qualitatively and quantitatively. The selective detection of H2S has been executed by introducing a simple chemodosimeter (PN-N3) that gives response on the basis of intramolecular charge transfer. UV–vis, fluorimetric, and NMR titrations were performed to demonstrate the sensing mechanism and electronic environment of PN-N3 in the presence of H2S. Density functional theory calculations were performed to validate the mechanism of azide (PN-N3) reduction to amine (PN-NH2) by the strong reducing power of H2S. The potentiality of this chemosensing method is that it could be treated as a simple, less-time-consuming, and cost-effective method for determining H2S in biological samples in the nanomolar range.

Rare Crystal Structure of Open Spirolactam Ring along with the Closed-Ring Form of a Rhodamine Derivative: Sensing of Cu2+ Ions from Spinach

“…The malignant behavior of Cu 2+ imposes a strong need for a reliable practical sensing method to selectively detect Cu 2+ ions in the living system. Among the various analytical methods, fluorescence chemosensors attract much more attention because of their simplicity, selectivity, low-cost, and real-time sensing. − To fulfill the purpose of a fluorescence turn-on chemosensor, rhodamine dyes are widely used due to their excellent spectroscopic properties, such as long wavelength excitation and emission profiles, large extinction coefficients, high fluorescence quantum yields, and the intense pink coloration after spirolactam ring opening. − Thus far, the work done for Cu 2+ sensing is mainly accomplished in live cell systems (comparison Table S1). We have now focused on plant tissues, which are one of the main sources of Cu 2+ accumulation in animals.…”

A turn-on fluorescent probe for selective detection of H2S in environmental samples and bio-imaging in human breast cancer cells

“…Consumption of H 2 S in the human body can also occur from our daily diet; hence, egg 51,52 was chosen for the quantitative estimation of H 2 S present in the food sample. For this purpose, the vapor that evolved after the separation and boiling of egg white were soaked in a filter paper coated with our probe TPB-NO 2 , and the process was continued for 1 hour to get the maximum possible concentration of H 2 S. The amount of H 2 S generated from the egg sample was then measured on the basis of fluorometric analysis.…”

Reaction-Based Luminescent Probes for Reactive Sulfur, Oxygen, and Nitrogen Species: Analytical Techniques and Recent Progress

“…Hydrogen sulfide has sparked vast creativity in the design of reaction-based triggers for this reactive analyte, and these can be generally categorized into reduction-based triggers, electrophilic triggers, copper-based triggers, and a few other interesting developments, including triggers incorporating molecular recognition (Figures – ). Reduction-based triggers include azides, − sulfonyl azides, , nitro groups, − nitroso groups, and quinones . A wide variety of electrophilic triggers that operate via hydrogen sulfide’s nucleophilicity have been developed including aldehyde-assisted ester cleavage, aldehyde-assisted conjugate addition, aldehyde-assisted dinitrophenyl cleavage, nucleophilic aromatic substitution, , nucleophilic addition to an imine, , nitrobenzoxadiazole with amine ,− or alcohol , leaving groups, vinyl chloride substitution alone or followed by cyclization, an aryl cyanate, disulfide-assisted ester cleavage, − diselenide-assisted ester cleavage,…”