CONSUMPTION OF Hibiscus sabdariffa DRIED CALYX ETHANOL EXTRACT IMPROVED REDOX IMBALANCE AND GLUCOSE PLASMA IN VITAMIN B12 RESTRICTION DIET IN RATS

Abstract

This study aimed to evaluate the effect of Hibiscus sabdariffa L. dried calyx ethanolic (HSE) extract on the redox imbalance and glucose plasma of vitamin B12 restriction Sprague-Dawley rats. The rat was fed a Vitamin B12 Restriction Diet for 16 weeks and treated with HSE as the treatment group. One group was fed a restriction diet not treated with HSE as a positive control group, and another was fed a control chow diet not treated with HSE as a negative control group. Vitamin B12, MDA, SOD activity enzyme, and glucose levels were evaluated in this study. The extract had a significant antioxidant capacity in terms of reducing the production of free radical scavenging activity. The HSE can repair the skewed redox imbalance and oxidative damage generated by a vitamin B12-deficient diet in the rat liver. The result of fasting glucose plasma levels, in 16 weeks showed both significantly different in all groups. In Sprague-Dawley rats with vitamin B12 restriction treated with HSE, a decrease in the glucose plasma in week sixteen was observed, together with a reduction of hepatic redox imbalance. Treatment with HSE protected hepatocytes from oxidative damage caused by vitamin B12 deficiency.

Effect of Roselle Flower Extract (Hibiscus sabdariffa Linn.) on Reducing Steatosis and Steatohepatitis in Vitamin B12 Deficiency Rat Model

“…We previously demonstrated that the dietary restriction of vitamin B12 causes oxidative stress, which is a state of cellular imbalance between free radicals and antioxidants, via the formation of homocysteine. The amino acid homocysteine, as previously studied, can cause oxidative stress by increasing the production of hydroxyl radicals [ 18 ]. Fats, proteins, carbohydrates and nucleic acids can all be oxidized by this radical group.…”

“…Our results showed that the roselle extracts improved the antioxidant defense against oxidative stress via the NRF2 signaling pathway. Previous research has demonstrated that rosella extract in the liver of vitamin B12-treated rats reduces lipid peroxidation production, and increases levels of antioxidant enzymes such as SOD [ 18 ]. In this study, the vitamin B12-restricted group supplemented with rosella extract expressed higher liver NRF2 levels than the group without rosella extract.…”

“…Their food intake was monitored daily, and theirweight was measured weekly. After 8 and 16 weeks, rats were fasted for 12 h and then euthanized; their blood and liver were collected for experimental testing, and their body weight was recorded [ 18 ]. Rat livers were dissected for histological analysis and stored at 80 °C until required for biochemical analysis.…”