Contact angle study of blood dilutions on common microchip materials

Pitts, Katie L.; Abu-Mallouh, S.; Fenech, Marianne

doi:10.1016/j.jmbbm.2012.07.007

Cited by 16 publications

(8 citation statements)

References 9 publications

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“…In this equation, P h is the pressure difference across the meniscus [ 15 ]. σ is the surface tension of the liquid (blood [ 16 ]), and θ A is the advancing contact angle (blood on acrylic [ 17 ]), while h and w are the height and width of the channel, respectively.…”

Section: Resultsmentioning

confidence: 99%

A Centrifugal Microfluidic Platform That Separates Whole Blood Samples into Multiple Removable Fractions Due to Several Discrete but Continuous Density Gradient Sections

2016

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We present a miniaturized centrifugal platform that uses density centrifugation for separation and analysis of biological components in small volume samples (~5 μL). We demonstrate the ability to enrich leukocytes for on-disk visualization via microscopy, as well as recovery of viable cells from each of the gradient partitions. In addition, we simplified the traditional Modified Wright-Giemsa staining by decreasing the time, volume, and expertise involved in the procedure. From a whole blood sample, we were able to extract 95.15% of leukocytes while excluding 99.8% of red blood cells. This platform has great potential in both medical diagnostics and research applications as it offers a simpler, automated, and inexpensive method for biological sample separation, analysis, and downstream culturing.

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Section: Resultsmentioning

confidence: 99%

A Centrifugal Microfluidic Platform That Separates Whole Blood Samples into Multiple Removable Fractions Due to Several Discrete but Continuous Density Gradient Sections

2016

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“…As the plasma is composed of water (90% <), proteins, and electrolytes, the contact angle of plasma on both the PDMS and polymer surfaces was relative to that of water with small deviation. 21) Indeed, the capillary pressure of plasma in a microchannel with a hydrogel pillar array is sufficient for driving the flow of a sample. Also, in a heterogeneous immunoassay in which an antibody is immobilized onto the detection zone of the substrate, it is required to keep the flow with a small volume of sample in the microchannel for the duration of reaction time sufficient for completing the antigen-antibody binding.…”

Section: Discussionmentioning

confidence: 99%

Hydrogel-based capillary flow pumping in a hydrophobic microfluidic channel

Bae

Lee

Yang

et al. 2014

Jpn. J. Appl. Phys.

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In this study, we propose a novel method to generate a capillary pressure-driven flow in a microchannel with a hydrophobic surface. The microfluidic device has a wide channel in which a hydrogel pillar array is embedded. The hydrogel pillar array was formed in the microchannel by a photopolymerization process. The flow rate due to a capillary action was strongly dependent on the distance between the pillars. Moreover, our capillary pumping with a hydrogel pillar array sustained the flow for more than 5 min with a limited sample volume. Our microfluidic device provides two advantages: (1) the modification of the polymer surface to make it hydrophilic is not required and (2) the conventional polymer molding technique can be applied to produce microfluidic devices, instead of the precision molding technique. The results indicate the possible fabrication of various microfluidic chip devices that can be easily implemented in point-of-care diagnostics.

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“…This sequence of photographs is captured as a video from a computer screen utilizing the video-capturing software Bandicam (Bandicam Company, Irvine, CA, USA). ImageJ ( , National Institute of Health, Bethesda, MD, USA) is used for image processing to identify whole blood, red blood cells, and plasma that is free from red blood cells [ 13 , 14 ].…”

Section: Methodsmentioning

confidence: 99%

Integrating Bio-Sensing Array with Blood Plasma Separation on a Centrifugal Platform

Peshin

Madou

Kulinsky

2023

Sensors

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Numerous immunoassays have been successfully integrated on disc-based centrifugal platforms (CDs) over the last 20 years. These CD devices can be used as portable point-of-care (POC) platforms with sample-to-answer capabilities where bodily fluids such as whole blood can be used as samples directly without pre-processing. In order to use whole blood as a sample on CDs, centrifugation is used to separate red blood cells from plasma on CDs. There are several techniques for using specific fluidic patterns in the centrifugal fluidic network, such as reciprocation, that enhances the sensitivity of the immunoassays, including those using microarray antigen membranes. Present work demonstrates, for the first time, simultaneous integration of blood plasma separation (BPS) and reciprocation on the CD platform. The integrated design allows plasma that is separated from the red blood cells in a sedimentation chamber to flow into the reciprocation chamber via a narrow connecting channel of 0.5 mm × 0.5 mm cross-section. Due to the small cross-section of the connecting channel, there is no inflow of the red blood cell into the reciprocation chamber during subsequent fluidic operations of the CD. While no inflow of the red blood cells into the reciprocation chamber was observed, the conditions of 20 g jerk acceleration were also simulated in ANSYS finite element analysis software, and it was found that the CD design that was used is capable of retaining red blood cells in the sedimentation chamber. Experimentally, the isolation of red blood cells in the sedimentation chamber was confirmed using the ImageJ image processor to detect the visible color-based separation of the plasma from the blood. A fluorescent analyte testing on the bio-sensing array of the presented novel integrated design and on the standard reciprocation design CD was conducted for 7 min of reciprocation in each case. The test analyte was Europium Streptavidin Polystyrene analyte (10−3 mg/mL) and the microarray consisted of Biotin bovine serum albumin (BSA) dots. The fluorescent signals for the standard and integrated designs were nearly identical (within the margin of error) for the first several minutes of reciprocation, but the fluorescent signal for the integrated design was significantly higher when the reciprocation time was increased to 7 min.

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Contact angle study of blood dilutions on common microchip materials

Cited by 16 publications

References 9 publications

A Centrifugal Microfluidic Platform That Separates Whole Blood Samples into Multiple Removable Fractions Due to Several Discrete but Continuous Density Gradient Sections

A Centrifugal Microfluidic Platform That Separates Whole Blood Samples into Multiple Removable Fractions Due to Several Discrete but Continuous Density Gradient Sections

Hydrogel-based capillary flow pumping in a hydrophobic microfluidic channel

Integrating Bio-Sensing Array with Blood Plasma Separation on a Centrifugal Platform

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