Contact guidance diversity in rotationally aligned collagen matrices

Nuhn, Jacob; Perez, Anai M.; Schneider, Ian C.

doi:10.1016/j.actbio.2017.11.039

Cited by 45 publications

(30 citation statements)

References 50 publications

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“…For aligned nanofiber scaffold, the aligned cell growth pattern makes the cell contact inhibition further reduced (Figure 7.g). (Nuhn, Perez & Schneider, 2018). Finally, we propose a possible mechanism of in vivo collagen matrix assembly guided by aligned ECCNNs with BMP-2, which involved 5 aspects: 1).…”

Section: Bone Regeneration Test In Vivomentioning

confidence: 97%

Aligned electrospun cellulose scaffolds coated with rhBMP-2 for both in vitro and in vivo bone tissue engineering

Zhang

Wang

Liao

et al. 2019

Carbohydrate Polymers

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Section: Bone Regeneration Test In Vivomentioning

confidence: 97%

Aligned electrospun cellulose scaffolds coated with rhBMP-2 for both in vitro and in vivo bone tissue engineering

Zhang

Wang

Liao

et al. 2019

Carbohydrate Polymers

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“…39 However, the robustness of the contact guidance differs among cells. 52 This diversity in response to this 2D contact guidance cue mimics behavior seen in 3D contact guidance cues 38 and in mouse models for invasion and metastasis 58 and has been used as a substrate by which to assess the role of adhesion and contractility in tuning contact guidance fidelity. 53 …”

Section: Introductionmentioning

confidence: 93%

Degradation and Remodeling of Epitaxially Grown Collagen Fibrils

et al. 2018

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Introduction— The extracellular matrix (ECM) in the tumor microenvironment contains high densities of collagen that are highly aligned, resulting in directional migration called contact guidance that facilitates efficient migration out of the tumor. Cancer cells can remodel the ECM through traction force controlled by myosin contractility or proteolytic activity controlled by matrix metalloproteinase (MMP) activity, leading to either enhanced or diminished contact guidance. Methods— Recently, we have leveraged the ability of mica to epitaxially grow aligned collagen fibrils in order to assess contact guidance. In this article, we probe the mechanisms of remodeling of aligned collagen fibrils on mica by breast cancer cells. Results— We show that cells that contact guide with high fidelity (MDA-MB-231 cells) exert more force on the underlying collagen fibrils than do cells that contact guide with low fidelity (MTLn3 cells). These high traction cells (MDA-MB-231 cells) remodel collagen fibrils over hours, pulling so hard that the collagen fibrils detach from the surface, effectively delaminating the entire contact guidance cue. Myosin or MMP inhibition decreases this effect. Interestingly, blocking MMP appears to increase the alignment of cells on these substrates, potentially allowing the alignment through myosin contractility to be uninhibited. Finally, amplification or dampening of contact guidance with respect to a particular collagen fibril organization is seen under different conditions. Conclusions— Both myosin II contractility and MMP activity allow MDA-MB-231 cells to remodel and eventually destroy epitaxially grown aligned collagen fibrils.

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“…Several methods including the use of magnetic beads [11,12] , application of mechanical strain [13,14] , cell-based compaction [15,16] , electrospinning [17,18] , and 3D printing approaches [19,20] have been used to create anisotropic collagen fibers in vitro. Even though current methods provide control over collagen fiber anisotropy, they are unable to combine these fiber domains with cellular scale control over physiologically relevant biophysical and biochemical cues that are possible using microfluidic approaches [21,22] .…”

Section: Introductionmentioning

confidence: 99%

Microengineered three-dimensional collagen fiber landscapes with independently tunable anisotropy and directionality

Ahmed

Joshi

Mansouri

et al. 2020

Preprint

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Fibrillar collagen is abundant in the extracellular matrix (ECM), and cellular processes including differentiation, proliferation, and migration, have been linked to the directionality and degree of alignment (anisotropy) of collagen fibers. Given the importance of cell-substrate interactions in driving biological functions, several microfluidic approaches have been developed to create threedimensional (3D) collagen gels with defined fiber properties that enable quantitative correlations between structural cues and observed cell responses. Although existing microfluidic methods provide excellent control over collagen fiber anisotropy, independent control over both fiber direction and anisotropy (that we collectively refer to as the "collagen landscape") has not been demonstrated. Therefore, to advance collagen microengineering capabilities, we present a userfriendly approach that uses fluid flows to create well-defined collagen landscapes within a microfluidic channel network. These landscapes include: i) control over fiber anisotropy, ii) spatial gradients in fiber anisotropy, and iii) defined fiber directionality. Finally, we present two approaches to simplify procedures to allow cell interaction with the microengineered collagen landscapes.

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Contact guidance diversity in rotationally aligned collagen matrices

Cited by 45 publications

References 50 publications

Aligned electrospun cellulose scaffolds coated with rhBMP-2 for both in vitro and in vivo bone tissue engineering

Aligned electrospun cellulose scaffolds coated with rhBMP-2 for both in vitro and in vivo bone tissue engineering

Degradation and Remodeling of Epitaxially Grown Collagen Fibrils

Microengineered three-dimensional collagen fiber landscapes with independently tunable anisotropy and directionality

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