Contact Transmission of Vaccinia to an Infant Diagnosed by Viral Culture and Metagenomic Sequencing

Martin, Rebekah M.; Burke, Kristina; Verma, Dinesh; Xie, Hai; Langer, Janine; Salais, Robert; Swaminathan, Sankar; Hanson, Kimberly E.

doi:10.1093/ofid/ofaa111

Cited by 6 publications

(4 citation statements)

References 18 publications

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“…Thus, a wide range of pathogens not covered by common NAATs can be detected through viral culture methods. 61 , 68 Furthermore, the viral variability or genetic mutations that may contribute to false NAATs negative results are less impacted by culture growth. 69 For different viruses infections, in situations of prolonged viral shedding, culture assays are important to determining virus infectivity and phenotypic antiviral susceptibility.…”

Section: Resultsmentioning

confidence: 99%

Monkeypox (Mpox) virus isolation and ultrastructural characterisation from a Brazilian human sample case

Miranda,

Caldas,

Ferreira

et al. 2023

Mem. Inst. Oswaldo Cruz

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BACKGROUND According to the last 2023 Monkeypox (Mpox) Outbreak Global Map from the Centres for Disease Control and Prevention (CDC), more than 100 countries with no Mpox infection report cases. Brazil stands out in this group and is the second country with the highest number of cases in the last outbreak. OBJECTIVE To contribute to knowledge of the virus infection effects in a cellular model, which is important for diagnosis infections not yet included in a provider´s differential diagnosis and for developing viral inhibition strategies. METHODS We describe a virus isolation protocol for a human clinical sample from a patient from Brazil, the viral growth in a cell model through plaque forming units (PFU) assay, reverse transcriptase polymerase chain reaction (RT-PCR) and transmission electron microscopy (TEM). FINDINGS We follow the viral isolation in Vero cell culture from a Mpox positive clinically diagnosed sample and show the infection effects on cellular structures using a TEM. MAIN CONCLUSIONS Understanding the impact of viral growth on cellular structures and its replication kinetics may offer better strategies for the development of new drugs with antiviral properties.

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Section: Resultsmentioning

confidence: 99%

Monkeypox (Mpox) virus isolation and ultrastructural characterisation from a Brazilian human sample case

Miranda,

Caldas,

Ferreira

et al. 2023

Mem. Inst. Oswaldo Cruz

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“…While conventional viral culture is more laborious and resource intensive, its utility during novel pathogen outbreaks should not be dismissed ( 11 ). Viral culture can detect a wide range of pathogens not covered by common NAATs and isolate viruses that may not have been part of a provider’s original differential diagnosis ( 12 ). Furthermore, culture growth is less impacted by strain variability or genetic mutations that may contribute to false negative results by NAATs ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Cytopathic Effects of Monkeypox Virus Isolated from Clinical Specimens and Differentiation from Common Viral Exanthems

Langer

Hanson

et al. 2022

J Clin Microbiol

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“…Viral culture can detect a wide range of pathogens not covered by common NAATs and isolate viruses that may not have been part of a provider’s original differential diagnosis. (11). Furthermore, culture growth is less impacted by strain variability or genetic mutations that may contribute to false negative results by NAATs (12).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the cytopathic effect of monkeypox virus isolated from clinical specimens and differentiation from common viral exanthems

Hanson

Langer

et al. 2022

Preprint

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While the practice of viral culture has largely been replaced by nucleic acid amplification tests, circumstances still exist in which the availability of viral culture will allow for the diagnosis of infections not included in a provider's differential diagnosis. Here, we examine the cytopathic effect (CPE) and clinical data associated with eighteen cases of monkeypox virus (MPXV) isolated from nineteen clinical samples submitted for viral culture. During the study period a total of 3,468 viral cultures were performed with herpes simplex virus most commonly isolated (646/3,468; 18.6%), followed by monkeypox virus (19/3,468; 0.6%) and varicella zoster virus (12/3,468; 0.4%). Most MPXV-positive samples were obtained from males (14/19) and taken from genital (7/19) or rectal lesions (5/19). Cycle threshold values of tested samples ranged from 15.3 to 29.0. Growth of MPXV in cell culture was rapid, yielding detectable CPE at a median of 2 days (range: 1-4) often with >50% of the monolayer affected in RMK, BGM, A549, and MRC-5 cell lines. As clinical features of MPXV, HSV, and VZV lesions may overlap, CPE patterns were comparted between viruses. HSV CPE developed in a similar time frame (median: 2 days, range: 1-7) but was more often negative in RMK cells relative to MPXV. VZV grew more slowly (median: 9 days, range: 5-11) and demonstrated CPE affecting ≤25% of cell monolayers when positive. Viral culture remains an important tool for the detection of rare or emerging viral pathogens, particularly when high viral load specimens are easily obtained.

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Contact Transmission of Vaccinia to an Infant Diagnosed by Viral Culture and Metagenomic Sequencing

Cited by 6 publications

References 18 publications

Monkeypox (Mpox) virus isolation and ultrastructural characterisation from a Brazilian human sample case

Monkeypox (Mpox) virus isolation and ultrastructural characterisation from a Brazilian human sample case

Characterization of the Cytopathic Effects of Monkeypox Virus Isolated from Clinical Specimens and Differentiation from Common Viral Exanthems

Characterization of the cytopathic effect of monkeypox virus isolated from clinical specimens and differentiation from common viral exanthems

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