Contactless magneto-permeabilization for intracellular plasmid DNA delivery in-vivo

Kardos, Thomas J.; Rabussay, Dietmar

doi:10.4161/hv.21576

Cited by 47 publications

(46 citation statements)

References 20 publications

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“…The result is agreement with the results of Towhidi et al (2012) and Kardos & Rabussay (2012). At the same time, the percentage of permeabilized cells after the PEMF treatment was relatively high (up to 70%), but the fluorescence intensity was low.…”

Section: Discussionsupporting

confidence: 91%

Membrane permeabilization of mammalian cells using bursts of high magnetic field pulses

Novickij

Dermol

Grainys

et al. 2017

PeerJ

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BackgroundCell membrane permeabilization by pulsed electromagnetic fields (PEMF) is a novel contactless method which results in effects similar to conventional electroporation. The non-invasiveness of the methodology, independence from the biological object homogeneity and electrical conductance introduce high flexibility and potential applicability of the PEMF in biomedicine, food processing, and biotechnology. The inferior effectiveness of the PEMF permeabilization compared to standard electroporation and the lack of clear description of the induced transmembrane transport are currently of major concern.MethodsThe PEMF permeabilization experiments have been performed using a 5.5 T, 1.2 J pulse generator with a multilayer inductor as an applicator. We investigated the feasibility to increase membrane permeability of Chinese Hamster Ovary (CHO) cells using short microsecond (15 µs) pulse bursts (100 or 200 pulses) at low frequency (1 Hz) and high dB/dt (>106 T/s). The effectiveness of the treatment was evaluated by fluorescence microscopy and flow cytometry using two different fluorescent dyes: propidium iodide (PI) and YO-PRO®-1 (YP). The results were compared to conventional electroporation (single pulse, 1.2 kV/cm, 100 µs), i.e., positive control.ResultsThe proposed PEMF protocols (both for 100 and 200 pulses) resulted in increased number of permeable cells (70 ± 11% for PI and 67 ± 9% for YP). Both cell permeabilization assays also showed a significant (8 ± 2% for PI and 35 ± 14% for YP) increase in fluorescence intensity indicating membrane permeabilization. The survival was not affected.DiscussionThe obtained results demonstrate the potential of PEMF as a contactless treatment for achieving reversible permeabilization of biological cells. Similar to electroporation, the PEMF permeabilization efficacy is influenced by pulse parameters in a dose-dependent manner.

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Section: Discussionsupporting

confidence: 91%

Membrane permeabilization of mammalian cells using bursts of high magnetic field pulses

Novickij

Dermol

Grainys

et al. 2017

PeerJ

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“…However, in the past 5 years the first scientific contributions featuring positive results in the pulsed >2 T magnetic field range have been performed [11][12][13]. In 2012 Towhidi et al have successfully demonstrated that the CHO cells are successfully permeabilized by the pulsed magnetic field [14,15].…”

Section: Introductionmentioning

confidence: 98%

“…in 2013 [16]. In 2012 Kardos and Rabussay have used 50 -75 µs magnetic field pulses up to 4 T for intracellular plasmid DNA delivery in vivo [13]. In our previous works we have shown that the pulsed magnetic field causes irreversible cell membrane permeabilization [12,17].…”

Section: Introductionmentioning

confidence: 99%

Magneto-Permeabilization of Viable Cell Membrane Using High Pulsed Magnetic Field

et al. 2015

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In this work we present novel experimental data of the magnetic permeabilization of the mammalian cells in high pulsed magnetic fields up to 16.4 T. Two designs of the multilayer solenoid type pulsed inductors, which were adapted for the biological experiments are presented. The induced electric field is analyzed using the finite element method analysis. The experimental methodology and the pulsed magnetic field setups are overviewed. The experimental results with mouse myeloma cell line Sp2/0 in pulsed magnetic field after pulse bursts and single pulse treatment are presented. The SYTOX ® Green dye was used for the estimation of the permeabilization process efficacy. It has been shown that the 16.4 T sub-millisecond low (< 1.1×10 5 T/s) dB/dt magnetic field pulses do not cause any observable dye uptake changes using the proposed methodology. The short repetitive microsecond range pulses (dB/dt > 0.8×10 6 T/s) result in an average up to 2.6±0.5% increase of the dye uptake.

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“…The first successful in vivo pDNA electrotransfer was achieved in 1991 using newborn mice (Titomirov et al 1991). To avoid unwanted electrode contact with the subject during electroporation, magnetic fields were generated using a hand-held flat magnet applicator to deliver green fluorescent protein-encoded pDNA to guinea pig skin in vivo (Kardos and Rabussay 2012). Magneto-permeabilization provides several advantages over electroporation: there is no need for invasive electrodes, it is more cost effective, and there is greater tissue penetration by the magnetic field.…”

Section: Plasmid Dna Administrationmentioning

confidence: 99%

Gene Therapy for Skin Diseases

Gorell

Ngôn

Lane

et al. 2014

Cold Spring Harbor Perspectives in Medicine

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Contactless magneto-permeabilization for intracellular plasmid DNA delivery in-vivo

Cited by 47 publications

References 20 publications

Membrane permeabilization of mammalian cells using bursts of high magnetic field pulses

Membrane permeabilization of mammalian cells using bursts of high magnetic field pulses

Magneto-Permeabilization of Viable Cell Membrane Using High Pulsed Magnetic Field

Gene Therapy for Skin Diseases

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