Contaminants from the Transplant Contribute to Intimal Hyperplasia Associated with Microvascular Endothelial Cell Seeding

Abstract

MVEC seeding in dogs results in intimal hyperplasia in all patent grafts, which contains myofibroblasts. Contaminants from the transplant contribute to this intimal hyperplasia.

Endothelial Cell Seeding Fails to Prevent Intimal Hyperplasia Following Arterial Injury in the Rat Carotid Model

“…The relationship between inflammatory processes and pronounced neointima formation has also been observed in other models of vascular disease [34]. At this moment we can only speculate that paracrine factors and / or (non-)cellular contaminants from the transplant contribute to the exacerbation of intimal hyperplasia in our model [35].…”

Endothelial vascular smooth muscle cell coculture assay for high throughput screening assays to identify antiangiogenic and other therapeutic molecules

“…However, microvascular cell cultures derived from adipose tissue are often contaminated with macrophages and fibroblasts which can produce responsible for intimal hyperplasia and inflammation upon reimplantation 69. Therefore extra precautions are needed to ensure a pure population of ECs when using adipose tissue.…”

Biological and engineering design considerations for vascular tissue engineered blood vessels (TEBVs)

“…Adipose-derived microvascular cell cultures are often contaminated with other cell types (e.g. macrophages and fibroblasts) resulting in an increased rather than decreased development of intimal hyperplasia in a dog model [12] and a decreased patency in transluminally seeded vessels in a rabbit model [13]. …”