Contamination of Fresh Produce with Antibiotic-Resistant Bacteria and Associated Risks to Human Health: A Scoping Review

Rahman, Mahbubur; Alam, Mahbub-Ul; Luies, Sharmin Khan; Kamal, Abul; Lin, Audrie; Sharior, Fazle; Khan, Rizwana; Rahman, Ziaur; Parvez, Sarker Masud; Amin, Nuhu; Hasan, Rashidul; Tadesse, Birkneh Tilahun; Taneja, Neelam; Islam, Mohammad Aminul; Ercümen, Ayşe

doi:10.3390/ijerph19010360

Cited by 60 publications

(47 citation statements)

References 59 publications

(128 reference statements)

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“…were indicated by investigators in the previous studies(Chau et al, 2017;Mercanoglu Taban and Halkman, 2011;Mir et al, 2018;Rahman et al, 2021). These results showed a relatively high contamination rate detected in the salad samples tested in this study, indicating that RTE salad serving in the meat restaurant is a risk factor for the transmission of food-borne diseases in humans (Bautista-De León et al, 2013).…”

supporting

confidence: 76%

Microbiological quality of ready-to-eat vegetable salads served at meat restaurants under the COVID 19 pandemic induced food demand: an example from Eastern Turkey

Baran

Adıgüzel

Aydın

2022

Preprint

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Ready-to-eat (RTE) salads service and utilize with pide/lahmacun, kebab types, and tantuni in meat restaurants in Turkish cuisine. Hence, the vegetable salads served with meat-based RTE foods are a risk factor for public health. In this study, we report the microbiological properties, antimicrobial resistance of foodborne pathogens including E. coli and S. aureus, and the presence of important enteric viruses in eastern Turkey. The total aerobic bacteria (TAB) ranged from < 1 to 6.40 log CFU g− 1 in the RTE salads (n = 42), whereas yeast and molds was ranged from < 1 to 6.26 log CFU g− 1. The coliform bacteria and the Staphylococcus and Micrococcus spp. count were determined < 1-5.82 log CFU g− 1 and < 1-5.66 log CFU g− 1 in all REVS samples, respectively. None of the RTE vegetable salads (REVS) tested in this study contained Salmonella spp., whereas E. coli and S. aureus were isolated in 38.1% (16/42) and 2.4% (1/42), respectively. S. aureus was resistant to gentamicin, kanamycin, aztreonam, and ciprofloxacin in the disc diffusion assay, however, it was not harboring the mecA gene. E. coli strains (n = 16) were resistant (100%) to aminoglycoside antibiotics (gentamicin and kanamycin) and 35.7% (6/16) of the isolates were ESBL producing. BlaTEM and blaCTXM8/25 were detected in two isolates, whereas one isolate carried blaCTXM−1 and blaTEM together by PCR. Of the REVS, two were evaluated as positive for rotavirus (4.8%), and six for hepatitis A (14.3%) and hepatitis E virus (14.3%). These results indicate the high microorganism load, presence of ESBL E. coli, and viral enteric pathogens in REVS, hence it is important to perform routine hygiene practices.

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supporting

confidence: 76%

Microbiological quality of ready-to-eat vegetable salads served at meat restaurants under the COVID 19 pandemic induced food demand: an example from Eastern Turkey

Baran

Adıgüzel

Aydın

2022

Preprint

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“…The methods aimed at new species-specific gene targets were particular and sensitive. Vegetables from retail markets and supermarkets were widely contaminated by P. aeruginosa and have resistant or reduced susceptibilities antibiotic ( Rahman et al, 2022 ). Pseudomonas aeruginosa as spoilage organisms in the ready-to-eat vegetables was distinguished by its capability to persist in highly antibiotic-resistant biofilm accumulation, which seriously affects shelf life ( Allydice-Francis and Brown, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

“…Studies found the ready-to-eat vegetables that were a potential-although rare-vector for colistin- and carbapenem-resistant P. aeruginosa , the contamination rate of P. aeruginosa has reached 17.5% or 34% ( Cai et al, 2015 ; Hölzel et al, 2018 ; Kapeleka et al, 2020 ; Junaid et al, 2021 ). That is to say, P. aeruginosa is a major contaminant of fresh vegetables, which might be a source of infection for susceptible persons within the community ( Rahman et al, 2022 ). Transmission of P. aeruginosa along the food chain could cause gastrointestinal infections ( Fakhkhari et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…Illumina whole gene sequencing has great advantages in accuracy, was used to analyze the distribution of P. aeruginosa after pasteurized milk, but it takes a lot of testing costs ( Maske et al, 2021 ). Furthermore, 25 articles mentioned health risks from consuming fresh produce by antimicrobial-resistant bacteria, but none quantified the risk ( Rahman et al, 2022 ). When the concentration of P. aeruginosa reaches a certain value, it may have the risk of colonization, so it is necessary to quantify its concentration ( Kwok et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pseudomonas aeruginosa Detection Using Conventional PCR and Quantitative Real-Time PCR Based on Species-Specific Novel Gene Targets Identified by Pangenome Analysis

Wang

Ai-ming

et al. 2022

Front. Microbiol.

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Mining novel specific molecular targets and establishing efficient identification methods are significant for detecting Pseudomonas aeruginosa, which can enable P. aeruginosa tracing in food and water. Pangenome analysis was used to analyze the whole genomic sequences of 2017 strains (including 1,000 P. aeruginosa strains and 1,017 other common foodborne pathogen strains) downloaded from gene databases to obtain novel species-specific genes, yielding a total of 11 such genes. Four novel target genes, UCBPP-PA14_00095, UCBPP-PA14_03237, UCBPP-PA14_04976, and UCBPP-PA14_03627, were selected for use, which had 100% coverage in the target strain and were not present in nontarget bacteria. PCR primers (PA1, PA2, PA3, and PA4) and qPCR primers (PA12, PA13, PA14, and PA15) were designed based on these target genes to establish detection methods. For the PCR primer set, the minimum detection limit for DNA was 65.4 fg/μl, which was observed for primer set PA2 of the UCBPP-PA14_03237 gene. The detection limit in pure culture without pre-enrichment was 105 colony-forming units (CFU)/ml for primer set PA1, 103 CFU/ml for primer set PA2, and 104 CFU/ml for primer set PA3 and primer set PA4. Then, qPCR standard curves were established based on the novel species-specific targets. The standard curves showed perfect linear correlations, with R2 values of 0.9901 for primer set PA12, 0.9915 for primer set PA13, 0.9924 for primer set PA14, and 0.9935 for primer set PA15. The minimum detection limit of the real-time PCR (qPCR) assay was 102 CFU/ml for pure cultures of P. aeruginosa. Compared with the endpoint PCR and traditional culture methods, the qPCR assay was more sensitive by one or two orders of magnitude. The feasibility of these methods was satisfactory in terms of sensitivity, specificity, and efficiency after evaluating 29 ready-to-eat vegetable samples and was almost consistent with that of the national standard detection method. The developed assays can be applied for rapid screening and detection of pathogenic P. aeruginosa, providing accurate results to inform effective monitoring measures in order to improve microbiological safety.

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“…Namun, penelitian lain menemukan adanya bakteri Salmonella spp. pada sayur selada segar 27,28 . Pencemaran bakteri Salmonella spp.…”

Section: Hasil Dan Pembahasanunclassified

Hubungan Pengetahuan Keamanan Pangan dengan Higiene Penjual dan Kontaminasi Salmonella spp Pada Lalapan Mentah di Kecamatan Patrang

Irianti

Mufida

Shodikin

et al. 2022

Jurnal Kesehatan Lingkungan Indonesia

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Latar belakang: Berdasarkan data Wilayah Kerja Puskesmas Patrang didapatkan sebanyak 2964 kasus diare pada tahun 2020 di Kabupaten Jember. Diare dapat terjadi akibat infeksi bakteri Salmonella spp. yang menular secara oral fecal. Bakteri Salmonella spp seringkali mencemari bahan pangan terutama sayuran. Sementara itu, masyarakat Indonesia sering mengonsumsi sayuran mentah yang disebut dengan lalapan mentah. Kontaminasi bakteri pada lalapan mentah dapat disebabkan oleh kondisi higiene penjual yang kurang baik yang dipengaruhi oleh beberapa faktor, seperti pengetahuan keamanan pangan penjual. Penelitian ini bertujuan untuk mengetahui hubungan pengetahuan keamanan pangan dengan higiene penjual dan kontaminan Salmonella spp pada lalapan mentah di Kecamatan Patrang.Metode: Penelitian ini menggunakan metode observasional analitik dengan desain penelitian cross sectional, dan data dianalisis menggunakan uji Spearman Rank. Sampel ditentukan dengan teknik simple random sampling dengan jumlah sampel 30 penjual.Hasil: Hasil penelitian menemukan bahwa 83,3% penjual memiliki pengetahuan keamanan pangan yang baik, 46,7% penjual memiliki higiene personal yang cukup, dan 80% lalapan mentah terkontaminasi Salmonella spp. Pada uji analisis Spearman Rank tidak didapatkan hasil yang signifikan antara pengetahuan keamanan pangan dengan higiene penjual (0,721>0,05) dan tidak ada hasil yang signifikan antara pengetahuan keamanan pangan dengan Salmonella spp (0,235>0,05).Simpulan: Dapat disimpulkan bahwa tidak ada hubungan pengetahuan keamanan pangan dengan higiene penjual dan Salmonella spp pada lalapan mentah di Kecamatan Patrang. ABSTRACT Title: Relation of Food Safety Knowledge, Seller Hygiene, and Salmonella spp on Raw Lalapan in Patrang DistrictBackground: Based on data from the Patrang Health Center Work Area, there were 2964 cases of diarrhea in 2020 in Jember Regency. Diarrhea can occur due to infection with Salmonella spp. which is transmitted oral fecal. Salmonella spp can contaminate food especially vegetables. Meanwhile, Indonesian people often consume vegetables salad called raw lalapan. Raw lalapan contamination can caused by seller poor hygiene that influenced by several factors, such as their food safety knowledge. This study aims to determine the relation of food safety knowledge with seller hygiene and Salmonella spp contaminants on raw lalapan in Patrang District.Method: This study used an analytical observational method with a cross sectional study design, and the data were analyzed using Spearman Rank test. The sample was determined by a simple random sampling technique with a sample of 30 sellers.Result: The study found that 83.3% seller had good food safety knowledge, 46.7% seller had sufficient personal hygiene, and 80% of raw lalapan were contaminated with Salmonella spp. In the Spearman Rank analysis test, there were no significant results between food safety knowledge and seller hygiene (0.721>0.05) and no significant results between food safety knowledge and Salmonella spp contaminants (0.235>0 ,05).Conclusion: So, it can be concluded that there was no relation of food safety knowledge with seller hygiene and Salmonella spp contaminants in raw vegetables in Patrang District.

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Contamination of Fresh Produce with Antibiotic-Resistant Bacteria and Associated Risks to Human Health: A Scoping Review

Cited by 60 publications

References 59 publications

Microbiological quality of ready-to-eat vegetable salads served at meat restaurants under the COVID 19 pandemic induced food demand: an example from Eastern Turkey

Microbiological quality of ready-to-eat vegetable salads served at meat restaurants under the COVID 19 pandemic induced food demand: an example from Eastern Turkey

Pseudomonas aeruginosa Detection Using Conventional PCR and Quantitative Real-Time PCR Based on Species-Specific Novel Gene Targets Identified by Pangenome Analysis

Hubungan Pengetahuan Keamanan Pangan dengan Higiene Penjual dan Kontaminasi Salmonella spp Pada Lalapan Mentah di Kecamatan Patrang

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