Contamination of K-Sol Corneal Storage Medium With Propionibacterium acnes

Sieck, Eric A.; Enzenauer, Robert W.; Cornell, F. Michael; Butler, Cliff

doi:10.1001/archopht.1989.01070020085036

Cited by 9 publications

(3 citation statements)

References 28 publications

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“…Eye banks and surgeons cope with potential sources of infectious agents other than the donor, [32][33][34] and additional factors could have confounded the analysis. Adjunctive lens and vitreous procedures increase the risk of postkeratoplasty infection, 35 and ocular surface flora can enter the eye during or after surgery.…”

Section: Commentmentioning

confidence: 99%

Infectious Disease Risk Factors of Corneal Graft Donors

Hassan

Wilhelmus²,

Dahl³

et al. 2008

Arch Ophthalmol

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To determine how donor health status affects the risk of infection after corneal transplant.Methods: An adverse reaction surveillance registry was used to conduct a matched case-control study among transplanted donor corneas from January 1, 1994, to December 31, 2003. Cases comprised 162 reports of endophthalmitis after penetrating keratoplasty including 121 with microbial recovery, of which 59 had concordant donor and recipient microbial isolates. Two controls were matched to each case by surgery date. Conditional logistic regression estimated adjusted odds ratios with 95% confidence intervals according to the premortem status of decedent donors.Results: Postkeratoplasty endophthalmitis was associated with recent hospitalization (odds ratio, 2.84; 95% confidence interval, 1.61-4.98) and fatal cancer (odds ra-

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Section: Commentmentioning

confidence: 99%

Infectious Disease Risk Factors of Corneal Graft Donors

Hassan

Wilhelmus²,

Dahl³

et al. 2008

Arch Ophthalmol

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“…[24][25][26] According to Glasser's 27 review of studies from 1981 to 2001, the incidence of contamination in donor eyes ranged from 5.3% to 19.4%. Incidence rates were 4.0% to 4.9% for keratitis after corneal grafting and 0.1% to 0.77% for endophthalmitis after corneal transplantation.…”

Section: Endophthalmitis and Microbial Keratitismentioning

confidence: 45%

Eye Banking and Corneal Transplantation Communicable Adverse Incidents

Dubord

Evans

Macsai

et al. 2013

Cornea

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The standardization of definitions for adverse events and reactions will be necessary to support the prevention and transmission of disease. International classification of a unique identification system for donors will be increasingly important for vigilance and traceability in cross-national exportation of human cells, tissues, and cellular- and tissue-based products. Opportunities for continuous improvement exist as does the need for constant vigilance and surveillance.

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“…10,11 There are many potential contamination sources other than the donor eyes, particularly at the tissue-processing and storage levels. [12][13][14] In this study, we investigated a cluster of corneoscleral rims positive for Achromobacter spp., nonfermenting Gram-negative bacilli (NF-GNB), identified for more than a 6-month period at the Massachusetts Eye and Ear (MEE). A likely common source of contamination was a particular lot of eyewash solution used to rinse the eye globe during tissue processing in the eye bank.…”

mentioning

confidence: 99%

A Cluster of Corneal Donor Rim Cultures Positive for Achromobacter Species Associated With Contaminated Eye Solution

André¹,

Durand

Buckley

et al. 2020

Cornea

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Purpose: To investigate a cluster of corneoscleral rim cultures positive for Achromobacter species over a 6-month period at Massachusetts Eye and Ear.Methods: An increased rate of positive corneal donor rim cultures was noted at Massachusetts Eye and Ear between July and December 2017. Positive cultures were subjected to identification and antimicrobial susceptibility testing by phenotypic (MicroScan WalkAway) and genotypic (16S rDNA sequencing) methods. Samples of the eye wash solution (GeriCare) used in the eye bank were also evaluated. Antimicrobial activity of Optical-GS against Achromobacter spp. at 4°C and 37°C was assessed by time-kill kinetics assay.Results: Of 99 donor rims cultured, 14 (14.1%) grew bacteria with 11 (78.6%) due to uncommon nonfermenting Gram-negative bacilli. These had been identified by standard automated methods as Achromobacter (n = 3), Alcaligenes (n = 3), Ralstonia (n = 2), Pseudomonas (n = 2), and Stenotrophomonas (n = 1). Eight of these 11 isolates were subsequently available for molecular identification, and all were identified as Achromobacter spp. Six bottles of eyewash solution were evaluated and were positive for abundant Achromobacter spp. (3.4 × 10 5 ± 1.1 CFU/mL). Optisol GS had no bactericidal activity against Achromobacter spp. at 4°C after 24-hour incubation but was bactericidal at 37°C. None of the patients who had received the contaminated corneas developed postoperative infection.

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Contamination of K-Sol Corneal Storage Medium With Propionibacterium acnes

Cited by 9 publications

References 28 publications

Infectious Disease Risk Factors of Corneal Graft Donors

Infectious Disease Risk Factors of Corneal Graft Donors

Eye Banking and Corneal Transplantation Communicable Adverse Incidents

A Cluster of Corneal Donor Rim Cultures Positive for Achromobacter Species Associated With Contaminated Eye Solution

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