Contamination, Prevention and Control of Listeria monocytogenes in Food Processing and Food Service Environments

Tabit, Frederick Tawi

doi:10.5772/intechopen.76132

Cited by 8 publications

(14 citation statements)

References 55 publications

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“…Gould et al (2013) indicated that sick persons should not be allowed to process food because it can be a source of bacteria transfer. In most cases, food contamination happens during processing and handling [5]. Similarly, Madden et al (2018) identified Listeria monocytogenes in food processing industries in Ireland.…”

Section: Resultsmentioning

confidence: 99%

“…Although fruit consumption is considered as healthy, it is commonly viewed as a potential risk factor for infection with enteropathogens such as Salmonella and Escherichia coli O157, with recent outbreaks linked to lettuce, spinach and tomatoes [5][6][7]. The sources of contamination are varied and include the application of organic wastes to farm land as fertilizer, contamination of waters used for irrigation with faecal material, direct contamination by livestock, wild animals and birds and postharvest issues such as worker hygiene [7].…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Microbial Load on Fresh-Cut Pineapple in Koforidua (Ghana) Market

2019

ANFS

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Fresh-cut fruits are fruits that have been peeled, trimmed, chopped and packaged for consumers while still maintaining its freshness. Fresh-cut fruits are increasingly becoming popular with consumers because of its convenience. A major challenge faced in Ghana is the inappropriate method of processing to maintain the quality of the fresh-cut fruits. This may increase the incidence of microbial contamination which will then reduce the shelf-life of fresh-cut fruits as well as causing foodborne illness to consumers. This study was therefore conducted to assess the microbial loads on fresh-cut pineapple sold in Koforidua market in Ghana. Fresh-cut pineapples were obtained from three different vendors in Koforidua central market in Ghana. Pour plate method was used to analyze five microorganisms which include Total coliform bacterial, Escherichia coli, Salmonella, Yeast and moulds on the fresh-cut pineapple samples. The microbial counts were transformed into colony forming units (cfu) and analyzed using SPSS. Salmonella plates showed no growth of bacteria colonies, but total coliform bacteria, Escherichia coli, yeast and moulds showed growth on the pour plates. The analyses indicated that the microbial count among vendors were not significantly different (P>0.05). The total coliform count showed a range of 1.1×103 to 1.2×103 cfu, 1.0 ×103 cfu for Escherichia coli, yeast ranged from 1.2×103 to 4.7×103 cfu, moulds ranged from 0 to 2.5×103cfu forall the three vendors. All the values indicated for the microorganisms analyzed are above the acceptable limit of microorganisms in food. It is therefore concluded that fresh-cut fruits sold on koforidua market are not quality for consumption; however vendors need to be trained on proper conditioning of the fruits prior to consumption.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Assessment of Microbial Load on Fresh-Cut Pineapple in Koforidua (Ghana) Market

2019

ANFS

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“…Severe complications can lead to meningitis or sepsis. 47 Listeriosis is a significant public health concern, but if diagnosed early can be treated using antibiotics. 46 L. monocytogenes is ubiquitously found in natural environments and animal hosts, 47 and can affect livestock and wildlife populations.…”

Section: Listeria Monocytogenesmentioning

confidence: 99%

The Application of Bacteriophage Diagnostics for Bacterial Pathogens in the Agricultural Supply Chain: From Farm-to-Fork

Jones

Shield

Swift

2020

PHAGE

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Bacteriophages (phages) have great potential not only as therapeutics but as diagnostics. Indeed, they have been developed and used to diagnose and detect bacterial infections, primarily in human clinical settings. The ability to rapidly detect and control bacterial pathogens in agriculture is of primary importance to maintain food security, improve animal health, and prevent the passage of zoonotic pathogens into the human population. Culture-based detection methods are often labor-intensive, and require further confirmatory tests, increasing costs and processing times needed for diagnostics. Molecular detection methods such as polymerase chain reaction are commonly used to determine the safety of food, however, a major drawback is their inability to differentiate between viable and nonviable bacterial pathogens in food. Phage diagnostics have been proven to be rapid, capable of identifying viable pathogens and do not require cultivation to detect bacteria. Phage detection takes advantage of the specificity of interaction between phage and their hosts. Furthermore, phage detection is cost effective, which is vitally important in agricultural supply chains where there is a drive to keep costs down to ensure that the cost of food does not increase. The full potential of phage detection/diagnostics is not wholly realized or commercialized. This review explores the current use and potential future scope of phage diagnostics and their application to various bacterial pathogens across agriculture and food supply chains.

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“…The genus Listeria is ubiquitous in nature and occurs frequently on farms and in food processing, handling, and storage environments. 1 L. monocytogenes causes listeriosis, which is one of the most serious foodborne diseases in humans, especially in pregnant women, neonates, and immunocompromised adults. 2 The virulence potential of L. monocytogenes relies on several molecular determinants, including virulence genes responsible for the invasion of host cells (inlA, inlB, and iap), phagosomal escape (hlyA, plcA, and plcB) and positive regulatory factor A (PrfA).…”

Section: Introductionmentioning

confidence: 99%

Control of Virulent Listeria monocytogenes Originating from Dairy Products and Cattle Environment Using Marine Algal Extracts, Silver Nanoparticles Thereof, and Quaternary Disinfectants

El‐Zamkan

Hendy

Diab

et al. 2021

IDR

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Introduction: Listeria monocytogenes is an important foodborne pathogen of public-and animalhealth concern globally. The persistence of L. monocytogenes in the dairy-processing environment has multifactorial causes, including lack of hygiene, inefficient cleaning, and improper disinfection practices. Materials and Methods: A total of 300 dairy-product and environmental samples were collected from dairy-cattle facilities and local dairy shops and vendors in Qena, Egypt. Samples were screened for the incidence of Listeria spp. and to detect virulence determinants and disinfectant-resistance genes. Three marine algal species -Caulerpa racemosa, Jania rubens, and Padina pavonica -were collected from Hurghada on the Red Sea coast. Algal extracts were screened using gas chromatography-mass spectrometry. The antimicrobial activity of some marine algal extracts, nanoparticles derived therefrom, and some disinfectants against L. monocytogenes strains were assessed in vitro using agar-well diffusion and liquid-broth methods. The impact of P. pavonica extract on the growth and survival of virulent L. monocytogenes in cheese and whey were clarified. Results and Discussion: The incidence of L. monocytogenes in dairy products and environmental samples was 15.5% and 19%, respectively. The most common toxigenic gene profile found among the isolates was hlyA + -inlA + -prfA + . The sensitivity pattern of L. monocytogenes strains to disinfectant containing alkyl (C 12-16 ) dimethyl BAC was high compared to other tested quaternary ammonium compounds (QAC) disinfectants tested, which showed lower log reductions against resistant strains. The QAC disinfectant-resistance gene qacH was detected in 40% of the isolates. Potent bactericidal activity of a petroleum ether extract of P. pavonica and silver nanoparticles of P. pavonica were obtained against the virulent L. monocytogenes strain. The population of L. monocytogenes in cheese curd and whey after 14 days was reduced at a rate of 9 log CFU/g and 8 log CFU/mL, respectively due to the effect of P. pavonica extract. After 28 days of storage, L. monocytogenes was completely inactivated in those dairy products. Conclusion: P. pavonica extract showed promising antimicrobial properties, calling for further comprehensive studies prior to it being applied in the food industry to enhance the safety, quality, and shelf life of products and protect public health.

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Contamination, Prevention and Control of Listeria monocytogenes in Food Processing and Food Service Environments

Cited by 8 publications

References 55 publications

Assessment of Microbial Load on Fresh-Cut Pineapple in Koforidua (Ghana) Market

Assessment of Microbial Load on Fresh-Cut Pineapple in Koforidua (Ghana) Market

The Application of Bacteriophage Diagnostics for Bacterial Pathogens in the Agricultural Supply Chain: From Farm-to-Fork

Control of Virulent Listeria monocytogenes Originating from Dairy Products and Cattle Environment Using Marine Algal Extracts, Silver Nanoparticles Thereof, and Quaternary Disinfectants

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