Contemporaneous synthesis of multiple carbohydrate debranching enzymes from newly isolated Aspergillus fumigatus SKF-2 under solid state fermentation: A unique enzyme mixture for proficient saccharification of plant bioresources

Mondal, Subhadeep; Soren, Jyoti Prakash; Mondal, Joy; Rakshit, Subham; Halder, Suman Kumar; Mondal, Keshab Chandra

doi:10.1016/j.indcrop.2020.112409

Cited by 29 publications

(10 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The lignocellulolytic enzyme production ability of the strains was primarily checked on the respective plate assay technique, and the strains that have ability to produce multiple lignocellulolytic enzymes were chosen. 18 The primary isolates were pure cultured and subsequently grown in liquid media for the lignocellulolytic enzyme production following the protocol of Mondal et al 18 The fungal strain producing the highest volume of lignocellulosic enzymes was finally chosen. For identification of the fungal strain, genomic DNA was isolated using a fungal DNA isolation Kit (Himedia, India).…”

Section: Methodsmentioning

confidence: 99%

Application of Phytochemicals To Combat Fungal Pathogens of Pulses: An Approach toward Inhibition of Fungal Propagation and Invasin Activity

Mondal

Santra

Uddin

et al. 2022

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

The present study represented an innovative strategy for inactivating the secreted invasins (lignocellulolytic enzymes) of fungal phytopathogens using natural phytochemicals to combat fungal infection to the pulses. A fungal pathogen (Aspergillus niger SKP1) was isolated from the white lentil (Vigna mungo), which has the ability to synthesize different lignocellulolytic enzymes. An in silico docking study elucidated that quercetin, naringin, epigallocatechin gallate, curcumin, and cinnamic acid were the prime efficient phytochemicals to inhibit the activity of fungal invasive enzymes like endoglucanase, endo-1,4-β-xylanase, and glucoamylase. Considering this observation, extracted phytochemicals in different mixtures were applied to prevent growth of the isolated pathogen under in situ experimental studies. The minimal inhibitory concentrations (MIC 50 ) and minimal fungicidal concentration (MFC 50 ) values of the first mixture (naringenin, epicatechin gallate, and cinnamic acid) and second mixture (quercetin and curcumin) were 170 and 220 mg/L and 320 and 380 mg/L, respectively. The studied phytochemicals were established to be cytosafe when compared to the commercial fungicides. The seeds of the white lentil were subjected to 1 year of long-term storage with the two aforementioned combinatorial phytochemicals. Subsequent morphological and physiological analyses revealed the complete protection of the stored seeds from the fungal infection. The present work has enough potentiality for the storage of pulses using natural preservatives that circumvent the adverse effect of the chemical preservatives on the ecosystem.

show abstract

Section: Methodsmentioning

confidence: 99%

Application of Phytochemicals To Combat Fungal Pathogens of Pulses: An Approach toward Inhibition of Fungal Propagation and Invasin Activity

Mondal

Santra

Uddin

et al. 2022

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Acetyl mannan esterase: It is a debranching enzyme that causes galactoglucomannan to release its acetyl groups. Agaricus [72], Aspergillus [73,74], Fusarium [64,75], and Trichoderma [76 -79] are fungi that have been discovered to breakdown hemicellulose. Hemicellulases are produced mostly by gram-positive bacteria, such as Bacillus species [80,81] and Clostridia species [82,83].…”

Section: Hemicellulasesmentioning

confidence: 99%

Microbial Enzyme Systems in the Production of Second Generation Bioethanol

Soni¹,

Sharma²,

Soni³

2022

Preprint

View full text Add to dashboard Cite

The primary contributor to global warming has been the careless usage of fossil fuels. Urbanization's threat to the depletion of these resources has made it necessary to find alternatives due to the rising demand. Four different forms of biofuels are now available and constitute a possible replacement for fossil fuels. The first generation of biofuels is generated from the edible portion of biomass, the second generation is made from the non-edible portion of biomass, the third generation is made from algal biomass, and the fourth generation is made using molecular biology to improve the algal strain. Second generation biofuels are extremely important because they are derived from non-edible biomass, such as agricultural and agro-industrial wastes rich in cellulose, hemicellulose, pectin, and starch impregnated with lignin, and are hydrolyzed after delignification by physio-chemical or biological pretreatments using ligninases. There has been a lot of research on their production using chemical and enzymatic hydrolysis, but it has not been economically viable in comparison to first generation biofuels due to the formation of several inhibitors with chemical hydrolysis and the high cost of the enzymes. Furthermore, the need for multiple enzymes due to the various types of carbohydrates in the feedstocks makes the enzymatic process too expensive. This article examines the enzymes involved in the hydrolysis of feedstocks for the production of second generation bioethanol, a highly acceptable biofuel.

show abstract

“…Basados en la información extraída del repositorio de nomenclatura Index Fungorum (http://www.indexfungorum.org/names/Names.asp) la totalidad de las cepas identificadas en el presente estudio corresponden al filo Ascomycota, representando los subfilos Pezizomycotina y Saccharomycotina, enclaustrados en las clases Sordariomycetes, Eurotiomycetes, Dothideomycetes y Saccharomycetes, y asociados a los órdenes Hypocreales, Eurotiales, Pleosporales y Saccharomycetales. Las especies Trichoderma yunnanense, Purpureocillum lilacinum y Beauveria bassiana son hongos que se caracterizan por su efecto antagonista y son ampliamente utilizados para el control biológico (Hernández et al, 2019;Rui et al, 2020;Romero et al, 2020) y pertenecen al orden de los Hypocreales; Aspergillus fumigatus, Penicillium chrysogenum, Penicillium Rubens y Penicillium citrinum son especies productoras de metabolitos de uso farmacéutico y reconocidos reductores de azúcares de interés para la industria de los biocombustibles y hacen parte del grupo de los Eurotiales (Cabral et al, 2018;Ouephanit et al, 2019;Lina et al, 2019;Tejas et al, 2019;Lodha et al, 2020;Huber et al, 2020;Xianchun et al, 2020;Mondal et al, 2020); Bipolaris sivanesaniana, Laburnicola hawksworthii, Alternaria destruens y Curvularia pisi son especies vinculadas con efectos patógenos en diferencies especies vegetales (Manamgoda et al, 2014;Zhai et al, 2018) y son del orden de los Pleosporales; y en el orden de los Saccharomycetales se encuentra a Geotrichum silvícola.…”

Section: Análisis Filogenéticounclassified

“…Aspergillus fumigatus es un reconocido patógeno oportunista, causante de queratitis, reacciones alérgicas en las vías respiratorias y aspergilosis pulmonar (Nayak et al, 2018). También, tiene aplicaciones biotecnológicas por su capacidad de producir lovastatina que es un metabolito secundario fúngico frecuentemente utilizado con fines farmacéuticos para reducir los niveles de colesterol y eventos cardiovasculares, puede producir enzimas con características celulasas, --xilosidasa, FPasa, xilanasa, peptidasa y amilasa que tienen aplicación en diferentes sectores productivos como la industria de producción de bioetanol (Xianchun et al, 2020;Mondal et al, 2020). Las celulasas son un conjunto de enzimas hidrolíticas que descomponen la celulosa, mediante la actividad sinérgica de tres diferentes componentes, las endoglucanasa, exoglucanasa y la ß-glucosidasa.…”

Section: Potencial Biotecnológico De Los Hongos Identificadosunclassified

Identificación molecular de hongos filamentosos y su potencial biotecnológico

Contreras

Figueredo

2021

Biotecnol. sector agropecuario agroind.

View full text Add to dashboard Cite

Los hongos filamentosos son organismos frecuentemente utilizados en el desarrollo de alternativas para resolver desafíos en diferentes sectores productivos. Igualmente, representan una oportunidad competitiva de valor económico en sectores como el agrícola, alimenticio y farmacéutico como también en el industrial y de biocombustibles. Dando continuidad a investigaciones previas de la Universidad Francisco de Paula Santander (UFPS), se analizaron 25 muestras del banco de cepas para caracterización molecular e identificación taxonómica. Se obtuvo ADN de cada cepa con la técnica fenol-cloroformo. Mediante las técnicas de PCR y de iniciadores de espaciadores internos de transcrito (ETS) primer 4 y 5, se obtuvieron los amplicones que fueron secuenciados. Se identificaron cepas de los ordenes Hypocreales, Eurotiales, Pleosporales y Saccharomycetales con ejemplares de géneros como el Aspergillus, Curvularia; Purpureocillium, Penicillium y Trichoderma entre otros. Los hallazgos se exhiben en el cladograma evidenciando la proximidad filogenética como también relacionando los potenciales biotecnológicos para el desarrollo de bio insumos, productos farmacéuticos y biocatalizadores. Gracias al estudio y revisión en bases de datos se logró avanzar en la descripción de las capacidades biológicas de estos hongos hacia el desarrollo de productos o servicios con base biotecnológica y enfoque de investigación en fitopatología.

show abstract

Contemporaneous synthesis of multiple carbohydrate debranching enzymes from newly isolated Aspergillus fumigatus SKF-2 under solid state fermentation: A unique enzyme mixture for proficient saccharification of plant bioresources

Cited by 29 publications

References 24 publications

Application of Phytochemicals To Combat Fungal Pathogens of Pulses: An Approach toward Inhibition of Fungal Propagation and Invasin Activity

Application of Phytochemicals To Combat Fungal Pathogens of Pulses: An Approach toward Inhibition of Fungal Propagation and Invasin Activity

Microbial Enzyme Systems in the Production of Second Generation Bioethanol

Identificación molecular de hongos filamentosos y su potencial biotecnológico

Contact Info

Product

Resources

About