Contemporary aspects of cholesterol metabolism in cattle.

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Farm animals’ growth, development and realization of productive qualities are closely interrelated by enzymatic transformations of amino acids, proteins and other complex nitrogenous compounds. Aspartate aminotransferase (AsT) is involved in the metabolism of amino acids in the cycles of urea, tricarboxylic acids and gluconeogenesis. The level of AsT in the blood depends on many paratypical and genetic factors. The paper presents the results of a biochemical analysis of the AsT content in the blood serum of Holstein bulls aged 12-13 months, who are descendants of 4 breeding bulls. Blood sampling was carried out in animals with a live weight of 330-365 kg, bred in the conditions of a large livestock enterprise located in Western Siberia. The level of AsT in the blood serum of animals was determined by the Reitman-Frenkel colourimetric method using reagents “Transaminase AsT Novo” (manufacturer “Vector-Best” Russia). It was found that the average value of the AsT content in the groups of sons was lower than the generally accepted physiological norm and, for all descendants, was 22.44 ± 1.29 E/l. The reference interval for AsT based on the central 95% percentile with 90% confidence intervals was 12.29 (8.67...15.91) - 34.84(31.2... 38.5) E/l. The descendants of different fathers had different phenotypic variability of the indicator and differed in the level of AST. Thus, the descendants of the bull-producer Brio were characterized by a higher level compared to the sons of the bulls Malstrem and Fabio by 1.69 and 1.8 times, respectively. The revealed differences indicate the hereditary determination of the AsT level in Holstein cattle. The strength of the influence of the father’s factor was 0.18.

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Hereditary definiteness of aspartate aminotransferase level in Holstein cattle

Себежко

Konovalova

Korotkevich

et al. 2023

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Interbreed differences in the cholesterol profile in cattle in Western Siberia

Sebezhko,

Konovalova,

Korotkevich

et al. 2023

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The authors studied the leading indicators of the cholesterol profile of high-density lipoproteins (HDL-C), low-density lipoproteins (LDL-C) and total cholesterol (TC) in Black-and-White, Red Steppe and Holstein breeds of the second or third lactation, bred in large livestock farms—complexes on the territory of Western Siberia. Metabolism and concentration of lipid complexes of cholesterol status play a cardinal role in the homeostasis of cellular metabolic processes. The critical role of serum lipoproteins in cholesterol metabolism is well recognised, as is their role as predictors of cardiovascular disease. At the same time, the atherogenic effect of LRN-C is associated with the transfer of fatty acids, which act as energy substrates for ATP synthesis. Anti-atherogenic properties characterise HDL-C and have an antioxidant and anti-inflammatory effect. The concentration of HDL-C - was determined by the precipitation method of phosphotungstic acid with magnesium chloride; the level of LDL-C - by the enzymatic colourimetric direct method, total cholesterol - by the process of enzymatic hydrolysis and oxidation of CHOD-PAP using reagent kits “HDL-Cholesterol-Novo”, “LDLCholesterol-Novo-A”, “Cholesterol-Novo” (“Vector-Best, Russia. The content of OH and LDL-C in the blood serum of cows at the age of the second or third lactation bred in Western Siberia is higher than the generally accepted standard values, which reflects a different degree of adaptation to climatic conditions. Ranked series of breeds (p < 0.05) according to LDL-C: black-and-white → Holstein → red steppe, medians were 4.91; 2.77 and 2.24 mmol/l, respectively. Differences between the indicators of HDL-C and LDL-C in Black-and-White, Red Steppe and Holstein cows were established, which indicates the genetic determination of the level of lipoproteins in the blood serum. The strength of the influence of the cow breed factor on the level of HDL-C in blood serum was 29%, LDL-C - 17%.

Features of hematological status of cows of different breeds of Western Siberia

Sebezhko,

Korotkevich,

Konovalova

et al. 2024

Monitoring hematological parameters in dairy herds is central to assessing animal health and welfare. The main parameters of peripheral blood were studied in healthy cows of Holstein, black-and-white and red breeds at the age of 30-60 days of the 2nd lactation, raised in Western Siberia. Studies of erythrocyte, leukocyte and platelet parameters were carried out on a PCE 90Vet analyzer with smear microscopy for calculating the leukocyte formula. Standard methods of descriptive statistics or robust statistics were used. Intergroup comparisons were performed using one-way analysis of variance or the Krasker-Wallace test. Tukey or Dunn’s method was used for post hoc comparisons. When calculating reference intervals, we used the bootstrap method. The calculated average values of the erythrocyte, platelet and leukocyte components of the hemogram in most cases varied within physiological norms or were on the borderline. Animals of the Holstein breed had the most pronounced adaptive potential. Differences between breeds have been established in almost all studied indicators except the number of red blood cells, MCV, RDW, basophils, band neutrophils, and immunoreactivity index. Cows of the black-andwhite breed were characterized by the highest level of hematocrit (27.99 ± 0.31%), absolute number of eosinophils (Me = 0.207 ×109/l), platelets (279.5 ± 22.13 ×109/l), for red steppe hemoglobin (98.32±1.53 g/l), monocytes (0.29±0.01) and average platelet volume (6.42±0.08 fL). Certain reference intervals for the leukocyte profile of cows provide monitoring of health status, the ability to compare data with other breeds, and also allows for comparative monitoring between regions.