Content and Fluxes of Electrolytes

Jones, Allan W.

doi:10.1002/cphy.cp020211

Cited by 52 publications

(53 citation statements)

References 173 publications

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“…4 ' *° Increased content of Mg in the DOCA-treated group (table 4) is consistent with the concept of increased cellular protein since Mg is largely adsorbed to ani6nic charges in the cells. 18 This study has confirmed that a major alteration in vascular Na metabolism accompanies the development of DOCA-salt hypertension. An increase in TJS;, for active transport represents a major change in Na efflux.…”

Section: Cell Watersupporting

confidence: 69%

“…If n = 1, no interaction takes place, and equation 1 reduces to the Michaelis-Menton model. 18 The K-stimulated effluxes of M Na are given in figure 5. The effluxes were computed from the product of the K-stimulated rate constants (figs.…”

Section: K -L J +4mentioning

confidence: 99%

“…This adsorptive process also involves Ca and could explain the high levels of Ca in excess of that in the "CoEDTA space. 18 …”

Section: K -L J +4mentioning

confidence: 99%

“…18 This has its origins in the analysis of cooperative adsorption of ions to fixed charges," and is not put forth as a unique model. Other multiparameter models predict sigmoidal transport in red blood cells,**' " and could fit well within the standard errors associated with the "Na effluxes.…”

Section: Kinetic Parametersmentioning

confidence: 99%

“…This does not offer direct support for the hypothesis that increased [ N a^n during hypertension will increase cell calcium and contraction via the mass action of a NaCa exchange mechanism." The energy for the exchange diffusion of 3 Na is required to remove one Ca from the cell, 18 -* a hence the transmembrane ratio for calcium depends on [Na]'. A 10% increase in [Na] Mn would increase [Cajcn by 33%.…”

Section: Effects Of Altered Transportmentioning

confidence: 99%

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Kinetics of active sodium transport in aortas from control and deoxycorticosterone hypertensive rats.

Jones¹

1981

Hypertension

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SUMMARY The efflux of sodium (M Na) was measured in the aortas from control and deoxycorticosterone (DOCA) hypertensive rats. Measurements were also made of aortic water content, extracellular space, and electrolyte contents. The objective was to determine the kinetics for potassium ( A growing body of evidence has linked altered ionic transport by vascular smooth muscle to experimental hypertension. Radioisotopic studies from our laboratory have shown increased turnover of sodium, potassium, and chloride in aortas from spontaneously hypertensive rats (SHR). 1 "* These observations were later extended to rats made hypertensive with deoxycorticosterone acetate (DOCA). 4> • The ability of norepinephrine to increase ionic fluxes also showed a supersensitivity. • has demonstrated an increased exchange of sodium and potassium for lithium in tail arteries from DOCA-treated rats and SHR. These exchanges were mainly diffusional movements. Evidence from two laboratories using different methods therefore support the hypothesis that increased membrane permeability to small ions accompany DOCA and spontaneous hypertension in the rat.

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Section: Cell Watersupporting

confidence: 69%

Section: K -L J +4mentioning

confidence: 99%

“…This adsorptive process also involves Ca and could explain the high levels of Ca in excess of that in the "CoEDTA space. 18 …”

Section: K -L J +4mentioning

confidence: 99%

Section: Kinetic Parametersmentioning

confidence: 99%

Section: Effects Of Altered Transportmentioning

confidence: 99%

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Kinetics of active sodium transport in aortas from control and deoxycorticosterone hypertensive rats.

Jones¹

1981

Hypertension

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Sodium 22⁺ washout from cultured rat cells

Kino

Nakamura

Hopp

et al. 1986

Journal Cellular Physiology

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The washout of Na+ isotopes from tissues and cells is quite complex and not well defined. To further gain insight into this process, we have studied 22Na+ washout from cultured Wistar rat skin fibroblasts and vascular smooth muscle cells (VSMCs). In these preparations, 22Na+ washout is described by a general three-exponential function. The exponential factor of the fastest component (k1) and the initial exchange rate constant (kie) of cultured fibroblasts decrease in magnitude in response to incubation in K+-deficient medium or in the presence of ouabain and increase in magnitude when the cells are incubated in a Ca++-deficient medium. As the magnitude of the kie declines (in the presence of ouabain) to the level of the exponential factor of the middle component (k2), 22Na+ washout is adequately described by a two-exponential function. When the kie is further diminished (in the presence of both ouabain and phloretin) to the range of the exponential factor of the slowest component (k3), the washout of 22Na+ is apparently monoexponential. Calculations of the cellular Na+ concentrations, based on the 22Na+ activity in the cells at the initiation of the washout experiments, and the medium specific activity agree with atomic absorption spectrometry measurements of the cellular concentration of this ion. Thus, all three components of 22Na+ washout from cultured rat cells are of cellular origin. Using the exponential parameters, compartmental analyses of two models (in parallel and in series) with three cellular Na+ pools were performed. The results indicate that, independent of the model chosen, the relative size of the largest Na+ pool is 92-93% in fibroblasts and approximately 96% in VSMCs. This pool is most likely to represent the cytosol.

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