Content and State of Glutathione in the Tissues of the Eye

Recent investigations of the chemistry of rod vision (Wald, '51) suggest the desirability of exploring the retina by histochemical means in an attempt to relate structure with chemical function. To this end we have applied a number of histochemical techniques to retinas of frogs and rhesus monkeys. The results of these procedures are described and illustrated with reference to the several retinal layers. The significance of the histochemical reactions is discussed and an interpretation of some of them offered with respect to the structural and possible functional organization of the photoreceptor cells. MATERIALS AND METHODS The material consisted of about 40 eyes of normal young adult rhesus monkeys (Jlacaca mulatta) and about twice that number of eyes of frogs (Rana p i p i e m ) .More methods of fixation and modification of histochemical procedure were carried out on frogs' than on monkeys' retinas, because frogs' retinas are more readily available. Furthermore, the rods of the frog are very much larger than those of the monkey, which favors their use for a number of histochemical procedures.The monkeys' eyes were removed from freshly killed animals, bisected equatorially with a razor blade into anterior and posterior segments, and fixed in different ways. I n 'This investigation was aided by a grant from the Eugene Higgins Fund of Harvard University. The cost of reproducing the colored plate was met through a special gift to the Department of Anatomy for that purpose.

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Content and State of Glutathione in the Tissues of the Eye

Cited by 44 publications

References 13 publications

The Role of Glutathione and Glutathione S‐Transferases in Mercapturic Acid Biosynthesis

The Role of Glutathione and Glutathione S‐Transferases in Mercapturic Acid Biosynthesis

The demonstration of thiol groups in certain tissues by means of a new colored sulfhydryl reagent

The chemical morphology of the retina

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