Content of 5-alpha-reductase (type 1 and type 2) mRNA in dermal papillae from the lower abdominal region in women with hirsutism

Abstract

The results of our study suggest that testosterone increases expression of 5-alpha-reductase types 1 and 2 in dermal papillae from the lower abdominal region in patients with hirsutism.

Idiopathic hirsutism: local and peripheral expression of aromatase (CYP19A) and 5α-reductase genes (SRD5A1 and SRD5A2)

“…Our results did not replicate the positive correlation between the free serum testosterone and the SRD5A expression and decreased SRD5A gene. Both our study and Skalba et al (14) study need further validation of mRNA expression results.…”

“…Alternatively, androgens may have different effects on gene transcription, translation, or posttranslational processes in various body sites or even within same follicle. Ska1ba et al (14) investigated mRNA expression of SRD5A1 and SRD5A2 in dermal papillae from the lower abdominal skin in 42 patients with PCOS and IH. They found higher levels of SRD5A1 mRNA than SRD5A2 mRNA in both groups, and also they found a positive correlation between both SRD5A gene isotypes and concentrations of free serum testosterone.…”

Changes in serum free testosterone, sleep patterns, and 5‐alpha‐reductase type I activity influence changes in sebum excretion in female subjects

“…The primer pair for 5‐alpha‐reductase type I was identified from previous publications: SRD5A1F 5′‐TCGTCAGACGAACTCAGTG‐3′; SRD5A1R 5′‐TAGTGGACGAGGAACATGG‐3′ (Invitrogen) . The relative gene quantization was normalized to housekeeping gene 18S (Invitrogen) in the samples by the comparative CT method.…”

The Association Between Polycystic Ovary Syndrome and Its Dermatological Manifestations

“…It is an early manifestation of virilization and correlates closely with elevated testosterone production [37]. Increased action of 5α reductase leading to more formation of active metabolites of testosterone, particularly in hair follicles, was noted in some studies [38,39].…”