Content of endoplasmic reticulum and Golgi complex membranes positively correlates with the proliferative status of brain cells

Silvestre, David C.; Maccioni, Hugo J. F.; Caputto, Beatriz L.

doi:10.1002/jnr.21915

Cited by 11 publications

(14 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this regard, the levels found in our study imply a greater participation of phosphorus during the development of neural tissue (9). Silvestre et al also showed that total lipid phosphorus content is higher in the embryonic brain than in adult brain cells (16). As the developing brain contains superior concentrations of phosphate groups belonging to lecithins, cephalins and sphingomyelins, this could explain the different values found in our study.…”

Section: Implications Of Trace Elements Detected In Cerebral Organoidmentioning

confidence: 43%

Trace elements during primordial plexiform network formation in human cerebral organoids

Sartore

Cardoso

Lages

et al. 2016

Preprint

View full text Add to dashboard Cite

Systematic studies of micronutrients during brain formation are hindered by restrictions to animal models and adult post-mortem tissues. Recently, advances in stem cell biology have enabled recapitulation of the early stages of human telencephalon development. In the present work, we exposed cerebral organoids derived from human pluripotent stem cells to synchrotron radiation in order to measure how biologically valuable micronutrients are incorporated and distributed in the exogenously developing brain. Our findings indicate that elemental inclusion in organoids is consistent with human brain tissue and involves calcium, iron, phosphorus, potassium, sulfur, and zinc. Local trends in concentrations suggest a switch from passive to actively mediated transport across cell membranes. Finally, correlational analysis for pairs of elements shows spatially conserved patterns, suggesting they may physically associate, be stored in similar compartments or used in related biological processes. These findings might reflect which trace elements are important during human brain development and will support studies aimed to unravel the consequences of disrupted metal homeostasis for neurodevelopmental diseases, including those manifested in adulthood.PeerJ Preprints | https://doi.org/10.7287/peerj.preprints.2126v1 | CC BY 4.0 Open Access |

show abstract

Section: Implications Of Trace Elements Detected In Cerebral Organoidmentioning

confidence: 43%

Trace elements during primordial plexiform network formation in human cerebral organoids

Sartore

Cardoso

Lages

et al. 2016

Preprint

View full text Add to dashboard Cite

show abstract

“…In this regard, the levels found in our study imply a greater participation of P during the development of neural tissue (Rajan et al, 1997). Silvestre and colleagues (2009) also showed that total lipid P content is higher in the embryonic brain than in adult brain cells (Silvestre, Maccioni & Caputto, 2009). The developing brain contains superior concentrations of phosphate groups belonging to lecithins, cephalins, and sphingomyelins, this could explain the different values found in our study.…”

Section: Sr-xrf Is a Suitable Technique To Scan And Image Trace Elemesupporting

confidence: 44%

Peer Review #1 of "Trace elements during primordial plexiform network formation in human cerebral organoids (v0.2)"

Hare¹

2017

View full text Add to dashboard Cite

Systematic studies of micronutrients during brain formation are hindered by restrictions to animal models and adult post-mortem tissues. Recently, advances in stem cell biology have enabled recapitulation of the early stages of human telencephalon development in vitro. In the present work, we analyzed cerebral organoids derived from human pluripotent stem cells by synchrotron radiation X-ray fluorescence in order to measure biologically valuable micronutrients incorporated and distributed into the exogenously developing brain. Our findings indicate that elemental inclusion in organoids is consistent with human brain tissue and involves P, S, K, Ca, Fe and Zn. Occurrence of different concentration gradients also suggests active regulation of elemental transmembrane transport. Finally, the analysis of pairs of elements shows interesting elemental interaction patterns that change from 30 to 45 days of development, suggesting short-or long-term associations, such as storage in similar compartments or relevance for time-dependent biological processes. These findings shed light on which trace elements are important during human brain development and will support studies aimed to unravel the consequences of disrupted metal homeostasis for neurodevelopmental diseases, including those manifested in adulthood.

show abstract

“…No difference in the labeling of phospholipids was observed between both genotypes, even after the addition of recombinant c-Fos to the assay medium indicating that the membrane biosynthesis machinery produces membrane at a rate high enough to be independent of its activation by c-Fos. In line with this possibility is the observation that the content of endoplasmic reticulum and Golgi membranes, where the bulk of membrane lipid synthesis occurs, is significantly higher in developing brain that in adult brain (Figure 11C ) [ 36 ].…”

Section: Discussionmentioning

confidence: 85%

“…Previous studies have shown that at early embryonic stages of brain development in which high rates of proliferation occur, the content of Golgi and ER membranes is significantly higher than that in adult brain cells [ 36 ]. So, we examined the expression of GM130 as a marker of the Golgi apparatus and of GRP78 BiP as a marker of the ER in E14.5 c-fos +/+ and c-fos −/− embryos and adult mice brain.…”

Section: Resultsmentioning

confidence: 99%

Brain development is impaired inc-fos−/− mice

et al. 2015

Self Cite

View full text Add to dashboard Cite

c-Fos is a proto-oncogene involved in diverse cellular functions. Its deregulation has been associated to abnormal development and oncogenic progression. c-fos−/− mice are viable but present a reduction in their body weight and brain size. We examined the importance of c-Fos during neocortex development at 13.5, 14.5 and 16.5 days of gestation. At E14.5, neocortex thickness, apoptosis, mitosis and expression of markers along the different stages of Neural Stem Progenitor Cells (NSPCs) differentiation in c-fos−/− and wild-type mice were analyzed. A ∼15% reduction in the neocortex thickness of c-fos−/− embryos was observed which correlates with a decrease in the number of differentiated cells and an increase in apoptosis at the ventricular zone. No difference in mitosis rate was observed, although the mitotic angle was predominantly vertical in c-fos−/− embryos, suggesting a reduced trend of NSPCs to differentiate. At E13.5, changes in differentiation markers start to be apparent and are still clearly observed at E16.5. A tendency of more AP-1/DNA complexes present in nuclear extracts of cerebral cortex from c-fos−/− embryos with no differences in the lipid synthesis activity was found. These results suggest that c-Fos is involved in the normal development of NSPCs by means of its AP-1 activity.

show abstract

Content of endoplasmic reticulum and Golgi complex membranes positively correlates with the proliferative status of brain cells

Cited by 11 publications

References 37 publications

Trace elements during primordial plexiform network formation in human cerebral organoids

Trace elements during primordial plexiform network formation in human cerebral organoids

Peer Review #1 of "Trace elements during primordial plexiform network formation in human cerebral organoids (v0.2)"

Brain development is impaired inc-fos−/− mice

Contact Info

Product

Resources

About