Content of Osmolytes and Flavonoids under Salt Stress in Arabidopsis thaliana Plants Defective in Jasmonate Signaling

Yastreb, Т. О.; Kolupaev, Yu. Е.; Lugovaya, A. A.; Дмитриев, А. П.

doi:10.1134/s0003683816020186

Cited by 34 publications

(11 citation statements)

References 33 publications

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“…(jasmonate insensitive 1) had significantly lower resistance to salt stress and lower proline and sugar content than the wild type. The concentration of flavonoids demonstrated that MYC2 was involved in controlling the plant protection systems under salt stress [64]. In the present study, the expression patterns of differentially expressed genes (DEG) in the jasmonic acid signaling pathway were similar, being highly expressed in roots in the early stage (6 h) of salt stress, but not at other times (Fig.…”

Section: Changes In Signal Transduction Pathways Of Eight Plant Hormonessupporting

confidence: 54%

Expression of Genes Related to Plant Hormone Signal Transduction in Jerusalem artichoke (<em>Helianthus tuberosus</em> L.) Seedlings Under Salt Stress

Yue¹,

Jueyun²,

Wencai³

et al. 2021

Preprint

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Background: Jerusalem artichoke (Helianthus tuberosus L.) is tolerant to salinity stress and has high economic value. The salt tolerance mechanisms of Jerusalem artichoke are still unclear. Especially in the early stage of Jerusalem artichoke exposure to salt stress, the plant physiology, biochemistry and gene transcription are likely to undergo large changes. Elucidating these changes may be of great significance to understanding the salt tolerance mechanisms of it. Results: We obtained high-quality transcriptome from leaves and roots of Jerusalem artichoke exposed to salinity (300 mM NaCl) for 0 h, 6 h, 12 h, 24 h and 48 h, with 150,129 unigenes and 9023 DEGs (Differentially Expressed Genes). The RNA-seq data were clustered into time-dependent groups (nine clusters each in leaves and roots); gene functions were distributed evenly among the groups convergence. KEGG enrichment analysis showed the genes related to plant hormone signal transduction were enriched in almost all treatment comparisons. Under salt stress, genes belongs to PYL (abscisic acid receptor PYR / PYL family), PP2C (Type 2C protein phosphatases), GH3 (Gretchen Hagen3), ETR (ethylene receptor), EIN2/3 (ethylene-insensitive protein 2/3), JAZ (Genes such as jasmonate ZIM-domain gene) and MYC2 (Transcription factor MYC2) had extremely similar expression patterns. The results of qPCR of 12 randomly selected genes confirmed the accuracy of RNA-seq. Conclusions: Under the impact of high salinity (300mM) environment, Jerusalem artichoke in the seedling stage was difficult to survive for a long time, and the phenotype was severe in the short term. Based on the expression of genes on the time scale, we found that the distribution of gene functions in time is relatively even. Upregulation of the phytohormone signal transduction had a crucial role in the response of Jerusalem artichoke seedlings to salt stress, the genes of abscisic acid, auxin, ethylene, and jasmonic acid had the most obvious change pattern.

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Section: Changes In Signal Transduction Pathways Of Eight Plant Hormonessupporting

confidence: 54%

Expression of Genes Related to Plant Hormone Signal Transduction in Jerusalem artichoke (<em>Helianthus tuberosus</em> L.) Seedlings Under Salt Stress

Yue¹,

Jueyun²,

Wencai³

et al. 2021

Preprint

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“…Gh_D12G1163 (KCS1), which is considered as the rate-limiting key enzyme by which the substrate and tissue specificities of fatty acid elongation are also decided in higher plants (Xiao et al, 2016 ), may affect the ratio of oil and protein in cottonseed. The genes, Gh_D12G1160 and Gh_D12G1161, coding the basic helix-loop-helix (bHLH) DNA-binding family proteins, which have been identified and characterized functionally in many plants with a critical role in the control of various biological processes including growth, development, and responses to various stresses (Gangappa and Chattopadhyay, 2013 ; Yastreb et al, 2016 ), may be related to the development of ovule and even to the protein accumulation during the development of seed. The genes, Gh_D12G1164, Gh_D12G1166, and Gh_D12G1167 (D-arabinono-1,4-lactone oxidase family protein), are related to oxidoreductase activity, D-arabinono-1,4-lactone oxidase activity, FAD binding, and catalytic activity in sperm cells and hypocotyls.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Study Identifies Candidate Genes Related to Seed Oil Composition and Protein Content in Gossypium hirsutum L.

Yuan

Wang

et al. 2018

Front. Plant Sci.

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Cotton (Gossypium spp.) is a leading natural fiber crop and an important source of vegetable protein and oil for humans and livestock. To investigate the genetic architecture of seed nutrients in upland cotton, a genome-wide association study (GWAS) was conducted in a panel of 196 germplasm resources under three environments using a CottonSNP80K chip of 77,774 loci. Relatively high genetic diversity (average gene diversity being 0.331) and phenotypic variation (coefficient of variation, CV, exceeding 3.9%) were detected in this panel. Correlation analysis revealed that the well-documented negative association between seed protein (PR) and oil may be to some extent attributable to the negative correlation between oleic acid (OA) and PR. Linkage disequilibrium (LD) was unevenly distributed among chromosomes and subgenomes. It ranged from 0.10–0.20 Mb (Chr19) to 5.65–5.75 Mb (Chr25) among the chromosomes and the range of Dt-subgenomes LD decay distances was smaller than At-subgenomes. This panel was divided into two subpopulations based on the information of 41,815 polymorphic single-nucleotide polymorphism (SNP) markers. The mixed linear model considering both Q-matrix and K-matrix [MLM(Q+K)] was employed to estimate the association between the SNP markers and the seed nutrients, considering the false positives caused by population structure and the kinship. A total of 47 SNP markers and 28 candidate quantitative trait loci (QTLs) regions were found to be significantly associated with seven cottonseed nutrients, including protein, total fatty acid, and five main fatty acid compositions. In addition, the candidate genes in these regions were analyzed, which included three genes, Gh_D12G1161, Gh_D12G1162, and Gh_D12G1165 that were most likely involved in the control of cottonseed protein concentration. These results improved our understanding of the genetic control of cottonseed nutrients and provided potential molecular tools to develop cultivars with high protein and improved fatty acid compositions in cotton breeding programs through marker-assisted selection.

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“…Five-week-old plants of arabidopsis thaliana L. wild-type (Col-0) and coi1 and jin1 lines were used in experiments. Plants were grown in water culture on Hoagland medium with modifications [20] at 24/18 °C (day/night), illumination of 6000 lux and 10 h photoperiod [9]. MJ (Sigma-Aldrich, USA) at a concentration 50 μM was inserted into the culture medium and plants were incubated on it for 24 h. The optimal concentration of MJ was determined in preliminary experiments, where the effect of 10-200 μM MJ on growth parameters of Col-0 under the salt stress (150 mM) was evaluated.…”

Section: Methodsmentioning

confidence: 99%

“…to doi: https://doi.org/10.15407/ubj90.05.050 salt [7,8]. Earlier, we showed less effective functioning of stress-protective systems under conditions of salt stress in arabidopsis mutant jin1 treated with JA compared to wild-type plants [9]. However, the transcription factor JIN1/MYC2 is involved in the effects of not only JA but also abscisic acid (ABA) [10][11][12][13] and, probably, nitric oxide [14,15].…”

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confidence: 98%

Action of methyl jasmonate and salt stress on antioxidant system of Arabidopsis plants defective in jasmonate signaling genes

Yastreb¹,

Kolupaev²,

Shvidenko³

et al. 2018

Ukr.Biochem.J.

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role of jasmonate signaling in the regulation of stress-protective systems in arabidopsis under salt stress remains insufficiently studied. For its clarification, comparative studies with mutants lacking various protein components of jasmonate signaling are advisable. in this connection, effects of methyl jasmonate (mJ, 50 μm) and salt stress (NaCl, 150 mm) on functioning of antioxidant and osmoprotective systems of wild-type arabidopsis plants (Col-0) and ones defective in jasmonate signaling, namely coi1 (mutant for gene coding the protein Coi1, which participates in removal of repressor proteins of transcription factors of jasmonate signaling) and jin1 (mutant defective in gene encoding the transcription factor JiN1/mYC2, one of the key in jasmonate signaling), were investigated. salt stress inhibited growth of plants of all three genotypes. treatment with mJ before salt stress positively influenced only the growth of wild-type plants. in contrast to mutants coi1 and jin1, Col-0 plants treated with mJ, under conditions of salt stress, kept close to the control values of water and total chlorophylls content, and the content of carotenoids increased. the coi1 plants under normal conditions differed from wild-type plants and jin1 mutants by reduced activity of guaiacol peroxidase and catalase and increased proline content. treatment with mJ did not affect the activity of antioxidant enzymes and proline content in both mutants defective in jasmonate signaling. under salt stress, the activity of superoxide dismutase, catalase and guaiacol peroxidase, as well as the content of proline and anthocyanins, in wildtype plants treated with mJ, were significantly higher than in control plants. the role of jasmonate-dependent protective systems in resistance of arabidopsis plants to salt stress is discussed.

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Content of Osmolytes and Flavonoids under Salt Stress in Arabidopsis thaliana Plants Defective in Jasmonate Signaling

Cited by 34 publications

References 33 publications

Expression of Genes Related to Plant Hormone Signal Transduction in Jerusalem artichoke (<em>Helianthus tuberosus</em> L.) Seedlings Under Salt Stress

Expression of Genes Related to Plant Hormone Signal Transduction in Jerusalem artichoke (<em>Helianthus tuberosus</em> L.) Seedlings Under Salt Stress

Genome-Wide Association Study Identifies Candidate Genes Related to Seed Oil Composition and Protein Content in Gossypium hirsutum L.

Action of methyl jasmonate and salt stress on antioxidant system of Arabidopsis plants defective in jasmonate signaling genes

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