Background and AimsAntimicrobial resistance (AMR) is a global health threat. Moreover, incorrect and inappropriate drug prescription behavior is considered a fundamental risk factor. Thus, the present study aims to develop, implement, and evaluate the effectiveness of an educational program based on the multi‐theoretical model (MTM) in improving antibiotic prescription behavior in veterinary students of Iran.MethodsThe present study will include four phases including a qualitative phase, an instrument design and psychometric test phase, and a cross‐sectional, and an interventional phase. In the first phase, the sampling will be purposive with a maximum variety. The interviews will be conducted with a sample of veterinarians.ResultsThe data will be analyzed in MAXQDA 10. In the second phase, the face and content validity will be tested by a panel of experts as field specialists. A confirmatory factor analysis will be used to test construct validity, and Cronbach's alpha coefficient and intracluster correlation coefficient will be used to determine the internal consistency of the instrument. Then, at this stage, a number of veterinary students will be selected through a multi‐stage sampling method. In the cross‐sectional phase, another sample of veterinary students will complete a researcher‐made questionnaire. Then, Spearman's correlation coefficient test will be used to test the relationship between the two stages of behavior initiation and behavior continuation. The data will be analyzed in SPSS 22. In the third phase, some veterinary students will be selected through a census and will be randomly divided into a control and an intervention group. To collect data in the final phase, the researcher‐made questionnaire that was designed in the second phase of the study based on a multi‐theory model will be used to extract data. To compare demographic characteristics, compare the correlation between the constructs of the multi‐theory model with antibiotic prescribing behavior in the cross‐sectional phase and compare the scores of the constructs of the MTM in two intervention and control groups paired‐samples T test and independent‐samples T test will be used.ConclusionThe present study will aim to improve antibiotic prescription behavior in veterinary students based on a MTM. The findings can be used as a model for training students in clinical fields such as veterinary medicine and general medicine at university at a national level. After verification and approval by experts and university professors, we can expect a change in the educational curriculum to include instructions on how to write out prescriptions for students. There are hopes that the present study if conducted accurately and widely to help prevent AMR in livestock, humans, and society.