Contents of myosin heavy chains in denervated slow and fast rat leg muscles

Jakubiec-Puka, Anna; Ciechomska, Iwona A.; Morga, Joanna; Matusiak, Agnieszka

doi:10.1016/s0305-0491(99)00027-9

Cited by 54 publications

(48 citation statements)

References 32 publications

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“…To evaluate the functionality of the VIP receptors expressed in skeletal muscle and to validate the observation that only VPAC2R is expressed in skeletal muscle, skeletal muscles in organ bath were stimulated with either the VPACR-nonselective agonist VIP, the VPAC1R-selective agonist [K 15 ,R (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27), or the VPAC2R-selective agonist Ro-25-1553, and cAMP generation was evaluated. As can be seen in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Skeletal muscle atrophy can be initiated by a variety of stimuli including, for example, disuse, nerve damage, and glucocorticoid use (2,15,17,19,31,37,40). Atrophy induces multiple changes in skeletal muscle that are independent of the atrophy-inducing stimuli, including decreased protein synthesis, increased protein degradation, alterations in contractile and metabolic enzyme protein isozymes, loss of vasculature, and remodeling of the extracellular matrix (2,5,6,22,24,25,27). Atrophy and hypertrophy are conserved processes across mammalian species.…”

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confidence: 99%

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Activation of the vasoactive intestinal peptide 2 receptor modulates normal and atrophying skeletal muscle mass and force

Hinkle¹,

Donnelly²,

Cody³

et al. 2005

Journal of Applied Physiology

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Activation of the vasoactive intestinal peptide 2 receptor modulates normal and atrophying skeletal muscle mass and force

Hinkle¹,

Donnelly²,

Cody³

et al. 2005

Journal of Applied Physiology

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“…In Wistar-Kyoto rats, the EDL muscles contain in addition to the predominant IIb and IId MHC isoforms/type IIB and type IID fibers, 8 -20% IIa MHC/IIA fibers, and small amounts of MHCI/ type I fibers (13,17,47). In a previous study (2), we also found that type IIA and IIA/IID hybrid fibers were present in sizeable proportions in both EDL (9.6 and 17.3%) and SOL (7.5 and 8.2%) muscles of Wistar-Kyoto rats.…”

Section: Ajp-cell Physiolmentioning

confidence: 99%

“…mechanically skinned fibers; myosin heavy chain isoforms; lineage; sarcoplasmic reticulum; Ca 2ϩ and Sr 2ϩ sensitivity; Long-Evans hooded rat MAMMALIAN SKELETAL MUSCLE fibers display a broad spectrum of structural and functional characteristics determined by the complement of homologous, but not identical, molecular structures involved in the excitation-contraction-relaxation cycle (33,34,41). Cross-innervation (4, 6), denervation (16,17,26,28,37), and chronic low-frequency stimulation (7,20,33,34) experiments have produced compelling evidence that the pattern of neural stimulation plays a crucial role in determining the functional and/or structural properties of skeletal muscle (38). However, neural control of fiber phenotype does not extend to the entire complement of cellular structures responsible for muscle fiber function, as demonstrated by crossinnervation studies.…”

mentioning

confidence: 99%

Denervation produces different single fiber phenotypes in fast- and slow-twitch hindlimb muscles of the rat

Patterson

Stephenson²,

Stephenson³

2006

American Journal of Physiology-Cell Physiology

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son. Denervation produces different single fiber phenotypes in fast-and slow-twitch hindlimb muscles of the rat. Am J Physiol Cell Physiol 291: C518 -C528, 2006. First published April 12, 2006 doi:10.1152/ajpcell.00013.2006.-Using a single, mechanically skinned fiber approach, we tested the hypothesis that denervation (0 to 50 days) of skeletal muscles that do not overlap in fiber type composition [extensor digitorum longus (EDL) and soleus (SOL) muscles of Long-Evans hooded rats] leads to development of different fiber phenotypes. Denervation (50 day) was accompanied by 1) a marked increase in the proportion of hybrid IIB/D fibers (EDL) and I/IIA fibers (SOL) from 30% to Ͼ75% in both muscles, and a corresponding decrease in the proportion of pure fibers expressing only one myosin heavy chain (MHC) isoform; 2) complex muscle-and fiber-type specific changes in sarcoplasmic reticulum Ca 2ϩ -loading level at physiological pCa ϳ7.1, with EDL fibers displaying more consistent changes than SOL fibers; 3) decrease by ϳ50% in specific force of all fiber types; 4) decrease in sensitivity to Ca 2ϩ , particularly for SOL fibers (by ϳ40%); 5) decrease in the maximum steepness of the force-pCa curves, particularly for the hybrid I/IIA SOL fibers (by ϳ35%); and 6) increased occurrence of biphasic behavior with respect to Sr 2ϩ activation in SOL fibers, indicating the presence of both slow and fast troponin C isoforms. No fiber types common to the two muscles were detected at any time points (day 7, 21, and 50) after denervation. The results provide strong evidence that not only neural factors, but also the intrinsic properties of a muscle fiber, influence the structural and functional properties of a particular muscle cell and explain important functional changes induced by denervation at both whole muscle and single cell levels. mechanically skinned fibers; myosin heavy chain isoforms; lineage; sarcoplasmic reticulum; Ca 2ϩ and Sr 2ϩ sensitivity; Long-Evans hooded rat MAMMALIAN SKELETAL MUSCLE fibers display a broad spectrum of structural and functional characteristics determined by the complement of homologous, but not identical, molecular structures involved in the excitation-contraction-relaxation cycle (33,34,41). Cross-innervation (4, 6), denervation (16,17,26,28,37), and chronic low-frequency stimulation (7,20,33,34) experiments have produced compelling evidence that the pattern of neural stimulation plays a crucial role in determining the functional and/or structural properties of skeletal muscle (38). However, neural control of fiber phenotype does not extend to the entire complement of cellular structures responsible for muscle fiber function, as demonstrated by crossinnervation studies. For example, when the extensor digitorum longus (EDL) muscle of the rat, a typically fast-twitch muscle, was cross-innervated by the nerve of the soleus (SOL) muscle, a typically slow-twitch muscle, the twitch time course of the cross-innervated muscle remained considerably faster than the twitch of the typical SOL muscle, even 16 ...

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“…In this manner, in vivo studies in the rat and the human have shown that exercise changes muscle fiber characteristics. From the perspective of stress, severing a nerve controlling skeletal muscle not only decreases muscle weight, but also changes muscle fiber characteristics (7,14). Studies in which muscle blood flow was regulated using devices such as a tourniquet have shown that even low-intensity training caused marked muscle hypertrophy and increased muscle force (25).…”

Section: Introductionmentioning

confidence: 99%

Effects of stretching stimulation with different rates on the expression of MyHC mRNA in mouse cultured myoblasts

et al. 2007

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In vivo studies have shown that changes in the characteristics of skeletal muscle fiber are determined by type of exercise or training. These earlier studies on mechanical stimulation, however, have all employed stimulation applied at a constant intensity, and no studies appear to have investigated change with variation of intensity of stimulation. In this study, we investigated the characteristics and differentiation of myoblasts stretched at different rates. Myoblasts were stimulated at 3 different rates, and the numbers of cells and nuclei on days 1, 3, and 5 were compared. The myosin heavy chain (MyHC) mRNA expression level was also compared. We investigated expression of MyHC-perinatal to determine speed of differentiation of myoblasts, and expression of MyHC2b, 2d, and 2a to ascertain muscle cell characteristics. Counting cells and nuclei of myoblasts revealed clear promotion of differentiation with stretching. With rapid stretching, expression of MyHC-perinatal was high at first, but then showed a decrease. In terms of effect on muscle fiber characteristics, MyHC-2b, MyHC-2d, and MyHC-2a were high with rapid, medium, and slow stretching, respectively. This indicated that myoblast differentiation was promoted regardless of difference in stretching speed, with the myoblasts acquiring the muscle-fiber characteristics appropriate to each rate of stretching.Exercise and training are known to bring about changes in the characteristics of skeletal muscle fibers (26,27). Numerous studies of the human (2,3,4,12,13,17,20,24) have shown that skeletal muscle fiber characteristics change depending on the mode of exercise. Anaerobic exercise facilitates increases in fast-twitch muscle, while aerobic exercise facilitates increases in slow-twitch muscle. Studies using rats have reported that long-term low-intensity exercise increases slow-twitch muscle and decreases fast-twitch muscle (9,15). In this manner, in vivo studies in the rat and the human have shown that exercise changes muscle fiber characteristics. From the perspective of stress, severing a nerve controlling skeletal muscle not only decreases muscle weight, but also changes muscle fiber characteristics (7,14). Studies in which muscle blood flow was regulated using devices such as a tourniquet have shown that even low-intensity training caused marked muscle hypertrophy and increased muscle force (25). However, few in vitro studies have been conducted to support and clarify these findings. In vitro studies have attempted to ascertain the effects of exercise and training by applying different stimuli. The first study (18) removed cells from rat hindlimb and soleus muscle and applied electrical stimuli via a culture solution to measure expression of myosin heavy chain (MyHC) isoforms. In the second study (11), osteoblasts were compressed using glass boards to ascertain morphological and bio-

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Contents of myosin heavy chains in denervated slow and fast rat leg muscles

Cited by 54 publications

References 32 publications

Activation of the vasoactive intestinal peptide 2 receptor modulates normal and atrophying skeletal muscle mass and force

Activation of the vasoactive intestinal peptide 2 receptor modulates normal and atrophying skeletal muscle mass and force

Denervation produces different single fiber phenotypes in fast- and slow-twitch hindlimb muscles of the rat

Effects of stretching stimulation with different rates on the expression of MyHC mRNA in mouse cultured myoblasts

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