Continual and accurate home monitoring of uric acid in urine samples with uricase-packaged nanoflowers assisted portable electrochemical uricometer

Development of enzyme–inorganic hybrid nanoflower-modified electrodes and a smartphone-controlled electrochemical analyzer for point-of-care testing of salivary amylase in saliva

“…The potential of NFs in improving enzyme stability has been demonstrated in a previous study where uricase loaded onto NFs showed only a 20% decrease in activity after 7 days of storage at 55 °C. 34 It is worth noting that the formation of NFs is not limited by enzyme type and they can be combined with various enzyme molecules in different reactions.…”

“…It has been well-documented that enzyme-inorganic hybrid 3D NFs can substantially enhance the stability of the immobilized enzymes for various sensing applications. [34][35][36] In this study, AG and GDH played a crucial role in the NF synthesis by providing nucleation sites for the formation of copper phosphate crystals (Cu 3 (PO 4 ) 2 •3H 2 O) which are the key component of the as-prepared NFs. The amide group within the main chain of AG (Fig.…”

Electrochemical Sensor Nanoarchitectonics for Sensitive Detection of Uric Acid in Human Whole Blood Based on Screen-Printed Carbon Electrode Equipped with Vertically-Ordered Mesoporous Silica-Nanochannel Film

“…Thus, NH 2 -VMSF/ErGO/SPCE sensor exhibits higher sensitivity and lower LOD, which is mainly due to the further enrichment of nanochannels. The LOD obtained on NH 2 -VMSF/ErGO/SPCE was lower than that obtained on Nafion-modified SPCE [ 33 ], nanodiamond modified SPE [ 34 ], Uricase/graphene-incorporated chitosan/Prussian blue modified SPCE (Uricase/Chi-Gr/PB/SPCE) [ 35 ], uricase-inorganic hybrid nanoflowers-Au nanoparticles modified SPCE (UOx-NFs-AuNPs-SPCE) [ 36 ], and Co 3 O 4 -ErGO modified SPE (Co 3 O 4 -ErGO/SPE) [ 37 ]. The low LOD of the developed sensor lies in the dual amplification effects resulting from ErGO and nanochannels.…”

Role of pretty nanoflowers as novel versatile analytical tools for sensing in biomedical and bioanalytical applications

“…These results demonstrated that PUM had huge potential in practical home UA detection. 55 Acetylcholinesterase (AChE) inhibition-based biosensors are widely used for the analysis of organophosphorous pesticides (OPs) in vegetables, fruits and environmental samples. The catalytic activity of AChE was inhibited in the presence of trace amounts of OPs.…”