Continued in vitro growth of fibroblast-like cells (RBCF-1) derived from the caudal fin of the fish, Carassius auratus

Shima, Akihiro; Nikaido, Osamu; Shinohara, S; Egami, Nobuo

doi:10.1016/0531-5565(80)90035-2

Cited by 68 publications

(17 citation statements)

References 9 publications

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“…Nutritional and humoral effects on these aged cells were also reported by his group. Polyploidization of hepatocytes in aged mice, but not fish, was also confirmed by Shima's group (Shima, 1975(Shima, , 1978Shima and Sugahara, 1976 ;Shima and Egami, 1978;Shima et al, 1980), who insisted that DNA damages per DNA content did not increase with elevated ploidy in old mice.…”

Section: Hepatocytes and Nerve Cellssupporting

confidence: 50%

“…These include normal human embryo lung fibroblasts, TIG-1 (Ohashi et al, 1980), Werner syndrome patient skin fibroblasts , porcine or bovine endothelial and smooth muscle cells (Mitsui et al, 1984a), and fish cells (Shima et al, 1980). Data on the optimum condition for the cryopreservation of human diploid fibroblasts for aging has been published by Kondo and Yamamoto (1981), and Yamamoto, Kondo and Ohashi (1981).…”

Section: Relation Of In Vitro Cellular Aging To In Vivo Human Agementioning

confidence: 99%

See 1 more Smart Citation

IV-7 Mechanisms of Cellular Aging

Mitsui¹,

Hirokawa²

1984

Cell Struct. Funct.

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Section: Hepatocytes and Nerve Cellssupporting

confidence: 50%

Section: Relation Of In Vitro Cellular Aging To In Vivo Human Agementioning

confidence: 99%

IV-7 Mechanisms of Cellular Aging

Mitsui¹,

Hirokawa²

1984

Cell Struct. Funct.

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“…RBCF-1 cells, derived from goldfish fin [22], were propagated as a monolayer culture at 37°C in Dulbecco's modified Eagle's (Gibco):-Ham F12 (1:1) medium supplemented with 10% FCS [23]. A mixture of 0.01 Ixg of receptor plasmid (pCMV-rtGR-I or II) and 0.2 ~tg of reporter plasmid (pMSG-CAT) were cotransfected into subconfluent cultures of RBCF-1 cells in each well of 6-well dishes (Falcon) using calcium phosphate coprecipitation [24,25].…”

Section: Transient Gr Expression In Cultured Cellsmentioning

confidence: 99%

Fish glucocorticoid receptor with splicing variants in the DNA binding domain

et al. 1996

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Here we describe the isolation of a rainbow trout cDNA containing an entire GR coding region. Although the encoded protein is highly homologous to other GRs, especially in its DNA binding domain, it contains a nine amino acid insertion between the two zinc fingers. This novel form is found in all rainbow trout tissues examined; however, the testis also contains a splice variant lacking this insert, making it completely continuous to other GRs. In transient transfection assays of cultured cells, the two rainbow trout GR variants activated transcription from the glucocorticoid-responsive mouse mammary tumor virus promoter to comparable levels.

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“…For example, the rainbow trout, Oncorhynchus mykiss, cell line RTG-2 cannot grow at 26-28ЊC and cannot survive at 30ЊC (Mosser et al 1986). It has been also reported that goldfish, Carassius auratus, cells easily change their growth rate in association with temperature shifts (Sato et al 1990): they maintain their proliferation even at 37ЊC (Shima et al 1980), terminate their growth at 40ЊC, and hardly survive at 42ЊC (Mitani et al 1989). Our previous study indicated that the population doubling times of goldfish cells at 30ЊC and 35ЊC were apparently shorter than those at 20ЊC and 25ЊC (Kondo and Watabe 2004).…”

Section: Introductionmentioning

confidence: 99%

Characterization of goldfish heat shock protein–30 induced upon severe heat shock in cultured cells

Kondo

Harano²,

Nakaya³

et al. 2004

Cell Stress Chaper

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Temperature-dependent changes of growth rate and protein components were investigated for primary cultured cells derived from goldfish caudal fin. When the culture temperature was shifted from 20ЊC to 35ЊC and 40ЊC, the growth rate was increased at 35ЊC as compared with that at 20ЊC, but no cell growth was observed at 40ЊC. The differential scanning calorimetry demonstrated the onset of the endothermic reaction for goldfish cellular components at 40ЊC. Therefore, the temperature shift to 40ЊC was found to be of severe heat shock for goldfish cultured cells. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that, although expression of 70-kDa components was slightly induced at 35ЊC, the temperature shift to 40ЊC markedly induced the expression of the 30-kDa component in addition to that of 70-kDa component. The N-terminal amino acid sequencing identified the 30-and 70-kDa components to be heat shock protein (Hsp)-30 and Hsp70, respectively. Northern blot analysis revealed that the enhanced Hsp30 messenger ribonucleic acid (mRNA) levels were only observed at 40ЊC, whereas Hsp70 mRNA was slightly accumulated at 35ЊC. These results indicated that Hsp30 might have important functions under severe heat stress condition.

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Continued in vitro growth of fibroblast-like cells (RBCF-1) derived from the caudal fin of the fish, Carassius auratus

Cited by 68 publications

References 9 publications

IV-7 Mechanisms of Cellular Aging

IV-7 Mechanisms of Cellular Aging

Fish glucocorticoid receptor with splicing variants in the DNA binding domain

Characterization of goldfish heat shock protein–30 induced upon severe heat shock in cultured cells

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