Continued initiation of DNA synthesis in arginine-deprived Chinese hamster ovary cells.

Weissfeld, Alice S.; Rouse, Harriet

doi:10.1083/jcb.73.1.200

Cited by 7 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, the susceptible tumour cell line, HeLa, continues to enter S-phase and traverse the cycle, albeit slowly. In agreement with Weissfeld and Rouse [8][9][10], synchronised HeLa cells took an inordinately long time to attain near G2 complements of DNA when released into S-phase in the absence of arginine ( Fig. 9a and b).…”

Section: Arginine Deprivation Cell Cycle Progression and Deathsupporting

confidence: 65%

“…Responses of human diploid fibroblasts, which have stringent regulation at the restriction point in G1 [6] , have been compared with HeLa cells. In contrast to isoleucine deprivation in CHO cells, which causes a G1 arrest [7], Weissfeld and Rouse [8][9][10] showed that KB cells continued to enter S-phase in arginine deficient medium. They paid little attention to effects beyond 48 h of total deprivation, and drew no comparisons with "normal" cells.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Single Amino Acid (Arginine) Restriction: Growth and Death of Cultured HeLa and Human Diploid Fibroblasts

Wheatley¹,

Scott²,

Lamb³

et al. 2000

Cell Physiol Biochem

View full text Add to dashboard Cite

Requirements for arginine are different from leucine for the growth of HeLa cells in monolayer and suspension culture. Cells grow increasingly more slowly as arginine levels fall below millimolar. Most cells died at 10^–5 M in static cultures, but could be sustained in perfused cultures, but at 10^–6 M neither perfusion nor increased volume in static cultures compensated. Cell died within 3-4 days in 10^–6 M in the same manner as those in complete arginine deprivation, i.e. considerably faster than with leucine deprivation. Arginine restriction produced by arginase or arginine decarboxylase addition to culture medium gave similar results. Citrulline substituted for arginine, but ornithine and polyamines did not.Arginine was depleted 3-4 times faster from the medium than other amino acids, <5% being consumed in protein synthesis, and arginine released by protein turnover was less efficiently reutilised than leucine. Deprivation reduced protein and DNA syntheses, greatly extended S-phase and protracted the cell cycle in HeLa cells for more than leucine deprivation. The inability of the cells avoid reinitiation of S-phase resulted in their proliferative impetus driving them into an late cycle (premitotic) death. In contrast, normal human diploid fibroblasts reached quiescence with little delay and survived for >11 days. Arginine deprivation is discussed as a selectively means of tumour cell destruction.

show abstract

Section: Arginine Deprivation Cell Cycle Progression and Deathsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Single Amino Acid (Arginine) Restriction: Growth and Death of Cultured HeLa and Human Diploid Fibroblasts

Wheatley¹,

Scott²,

Lamb³

et al. 2000

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Arginine is consumed at a relatively high rate by CHO cells in antibody production processes . Arginine starvation can cause CHO cells in exponential phase to be captured in the G0 or G1 phase . Excess arginine in the culture medium can effectively reduce the ratio of the acidic species of products.…”

Section: Key Components Of Mammalian Cell Culture Mediamentioning

confidence: 99%

“…81 Arginine starvation can cause CHO cells in exponential phase to be captured in the G0 or G1 phase. 82 Excess arginine in the culture medium can effectively reduce the ratio of the acidic species of products. However, unlike lysine, high arginine concentrations can inhibit cell growth and reduce viability.…”

Section: Amino Acidsmentioning

confidence: 99%

Cell culture media for recombinant protein expression in Chinese hamster ovary (CHO) cells: History, key components, and optimization strategies

Ritacco

Khetan

2018

Biotechnology Progress

192

138

View full text Add to dashboard Cite

The culture of Chinese Hamster Ovary (CHO) cells for modern industrial applications, such as expression of recombinant proteins, requires media that support growth and production. Such media must support high viable cell densities while also stimulating the synthesis and extracellular transport of biologic products. Early media development efforts in this area yielded basic formulations to sustain growth, viability, and cellular function, albeit comprising animal sourced components, and complex constituents used in batch culture mode. Subsequent improvements included the development of serum‐free and chemically defined (CD) media, the identification of critical nutrients, growth factors, and potentially inhibitory or toxic cellular metabolites, and the use of fed‐batch and perfusion culture techniques to optimize nutrient delivery while minimizing accumulation of unwanted waste products. This review is comprised of sections covering milestones in the evolution of mammalian cell culture media, nutrient composition and formulation requirements, optimization strategies, consistency and scalability of powder and liquid media preparation for industrial applications, and key recent advances driving progress in CHO cell culture medium design and development. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:1407–1426, 2018

show abstract

“…Deprivation of single amino acids including arginine induces severe chromosome abnormalities in cultured cells (Freed & Schatz, 1969). Growth inhibition by arginine depletion is associated with continued initiation of DNA synthesis (Weissfeld & Rouse, 1977) but severely depressed protein and RNA synthesis (Weinberg & Becker, 1970). This type of metabolic imbalance (although the other way round) is reminiscent of the effect of thymidine excess on cultured cells, which also causes severe chromosome damage (Yang et al, 1966) and is selectively cytotoxic for malignant cells (Lee et al, 1977).…”

Section: Effect Of Complete Arginine Deprivation On Cell Numbersmentioning

confidence: 99%

Differential arginine dependence and the selective cytotoxic effects of activated macrophages for malignant cells in vitro

Currie¹,

Basham²

1978

Br J Cancer

View full text Add to dashboard Cite

Summary.-Normal and neoplastic cells from 4 species (man, rat, mouse and hamster) were examined for their dependence on exogenous L-arginine in tissue culture. The malignant cells required a higher concentration of L-arginine in the medium than their normal counterparts (with similar doubling times) to maintain optimal proliferation. Complete arginine deprivation resulted in equal growth inhibition of normal and malignant cells, but more rapid cytolysis of the malignant cells. Deprivation of L-arginine, followed 24 h later by rescue with L-arginine, allowed normal cells to proliferate, but the reproductive capacity of the malignant cells was irreversibly impaired. Since the cytotoxic activity of LPS-activated macrophages was associated with the release of arginase and was abrogated by excess L-arginine, it is suggested that the biological basis for the selective effects of such macrophages may reside in the L-arginine dependence of the target cells.

show abstract

Continued initiation of DNA synthesis in arginine-deprived Chinese hamster ovary cells.

Abstract: When exponentially growing CHO cells were deprived of arginine (Arg), cell multiplication ceased after 12 h, but initiation of DNA synthesis continued: after 48 h of starvation with continuous [3H]thymidine exposure,

Cited by 7 publications

References 13 publications

Single Amino Acid (Arginine) Restriction: Growth and Death of Cultured HeLa and Human Diploid Fibroblasts

Single Amino Acid (Arginine) Restriction: Growth and Death of Cultured HeLa and Human Diploid Fibroblasts

Cell culture media for recombinant protein expression in Chinese hamster ovary (CHO) cells: History, key components, and optimization strategies

Differential arginine dependence and the selective cytotoxic effects of activated macrophages for malignant cells in vitro

Contact Info

Product

Resources

About