Continued use of MDA-MB-435, a melanoma cell line, as a model for human breast cancer, even in year, 2014

Prasad, Vidudala V T S; Gopalan, Ramprasad Og

doi:10.1038/npjbcancer.2015.2

Cited by 42 publications

(34 citation statements)

References 27 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The exact origin of MDA-MB-435s cells is under constant debate [70]. MDA-MB-435s was initially thought to be of breast cancer origin [71], and is still extensively used in breast cancer research [72–80].…”

Section: Resultsmentioning

confidence: 99%

Functional and prognostic significance of long non-coding RNA MALAT1 as a metastasis driver in ER negative lymph node negative breast cancer

et al. 2016

View full text Add to dashboard Cite

MALAT1 (metastasis associated lung adenocarcinoma transcript1) is a conserved long non-coding RNA, known to regulate gene expression by modulating transcription and post-transcriptional pre-mRNA processing of a large number of genes. MALAT1 expression is deregulated in various tumors, including breast cancer. However, the significance of such abnormal expression is yet to be fully understood. In this study, we demonstrate that regulation of aggressive breast cancer cell traits by MALAT1 is not predicted solely based on an elevated expression level but is context specific. By performing loss- and gain-of-function studies, both under in vitro and in vivo conditions, we demonstrate that MALAT1 facilitates cell proliferation, tumor progression and metastasis of triple-negative breast cancer (TNBC) cells despite having a comparatively lower expression level than ER or HER2-positive breast cancer cells. Furthermore, MALAT1 regulates the expression of several cancer metastasis-related genes, but displays molecular subtype specific correlations with such genes. Assessment of the prognostic significance of MALAT1 in human breast cancer (n=1992) revealed elevated MALAT1 expression was associated with decreased disease-specific survival in ER negative, lymph node negative patients of the HER2 and TNBC molecular subtypes. Multivariable analysis confirmed MALAT1 to have independent prognostic significance in the TNBC lymph node negative patient subset (HR=2.64, 95%CI 1.35 − 5.16, p=0.005). We propose that the functional significance of MALAT1 as a metastasis driver and its potential use as a prognostic marker is most promising for those patients diagnosed with ER negative, lymph node negative breast cancer who might otherwise mistakenly be stratified to have low recurrence risk.

show abstract

Section: Resultsmentioning

confidence: 99%

Functional and prognostic significance of long non-coding RNA MALAT1 as a metastasis driver in ER negative lymph node negative breast cancer

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The most recent consensus is that the line was indeed contaminated with melanoma cells and should not be used as a breast cancer model [25].…”

Section: Short Tandem Repeat (Str)-profiling and Targeted Mutation Anmentioning

confidence: 99%

Multidimensional phenotyping of breast cancer cell lines to guide preclinical research

Saunus

Smart

Kutasovic

et al. 2017

Breast Cancer Res Treat

View full text Add to dashboard Cite

Purpose Cell lines are extremely useful tools in breast cancer research. Their key benefits include a high degree of control over experimental variables and reproducibility. However, the advantages must be balanced against the limitations of modelling such a complex disease in vitro. Informed selection of cell line(s) for a given experiment now requires essential knowledge about molecular and phenotypic context in the culture dish.

show abstract

“…As for the MDA-MB-435 cell line used in this study, questions have been raised that gene expression analysis of the cells produced some microarrays in which MDA-MB-435 clustered with cell lines of melanoma origin [46][47][48]. We are aware of the debate about the origin of this cell line [49] and despite these speculations, strong evidence has been presented reiterating that MDA-MB-435 is of breast cancer origin [50] and may be used as "an excellent model for studies of highly malignant and dedifferentiated breast cancers" [47]. However, we realize that discussions concerning the cell line's genetic origin are outside the scope of this paper, which focus more on demonstrating the capability of high-resolution in vivo imaging of cancer using conductivity contrasts.…”

Section: Discussionmentioning

confidence: 99%

<italic>In Vivo</italic> Electrical Conductivity Contrast Imaging in a Mouse Model of Cancer Using High-Frequency Magnetoacoustic Tomography With Magnetic Induction (hfMAT-MI)

Shao

Ashkenazi

et al. 2016

IEEE Trans. Med. Imaging

View full text Add to dashboard Cite

Cancerous tissues have electrical-conductivity signatures different from normal tissues, which contain potentially useful information for early detection. Despite recent advancements in electrical-conductivity imaging and its applications, imaging electrical conductivities with high spatial resolution remains a challenge for non-invasive diagnosis of early-stage cancer. Among the various electrical-conductivity imaging methods, magnetoacoustic tomography with magnetic induction (MAT-MI) is a promising technology for non-invasive detection of breast cancer. However, previous efforts to use MAT-MI for cancer imaging have suffered due to insufficient spatial resolution. In this work, we have developed a high-frequency MAT-MI (hfMAT-MI) system with a 2-D spatial resolution of 1 mm, a significant improvement over previous methods. Furthermore, we demonstrated the performance of this method using an in vivo cancer model in nude mice with human breast xenograft hindlimb tumors. hfMAT-MI was able to resolve not only the boundaries between cancerous and healthy tissues, but also the tumors' internal structures. Importantly, we were able to track a growing tumor using our hfMAT-MI method for the first time in an in vivo mouse model, demonstrating the promise of this magneto-acoustic imaging system for effective detection and diagnosis of early-stage breast cancer.

show abstract

Continued use of MDA-MB-435, a melanoma cell line, as a model for human breast cancer, even in year, 2014

Cited by 42 publications

References 27 publications

Functional and prognostic significance of long non-coding RNA MALAT1 as a metastasis driver in ER negative lymph node negative breast cancer

Functional and prognostic significance of long non-coding RNA MALAT1 as a metastasis driver in ER negative lymph node negative breast cancer

Multidimensional phenotyping of breast cancer cell lines to guide preclinical research

<italic>In Vivo</italic> Electrical Conductivity Contrast Imaging in a Mouse Model of Cancer Using High-Frequency Magnetoacoustic Tomography With Magnetic Induction (hfMAT-MI)

Contact Info

Product

Resources

About