Continuous adsorption and photothermal lysis of airborne bacteria using a gold-nanoparticle-embedded-geometrically activated surface interaction (gold-GASI) chip.

Kwon, Kirok; Park, Ji Woon; Hyun, Kyung A.; Kwak, Byung Kook; Dan, Yong; Hwang, Jungho; Jung, Hyo Il

doi:10.1016/j.snb.2017.04.043

Cited by 14 publications

(4 citation statements)

References 20 publications

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“…The microfluidic chips with staggered herringbone structures were often reported to collect the bioaerosols with high capture efficiency. − First, the bioaerosols were continuously sucked into a microfluidic chip with staggered herringbone structures using a pump. Then, the primarily laminar-flow bioaerosols were chaotic vortexed by the staggered herringbone structures to boost the rotating and stretching of the bioaerosols, thus greatly enhancing the contact opportunity between the bioaerosols and the wall of the microchannel.…”

Section: Bioaerosol Collectionmentioning

confidence: 99%

Recent Advances on Bioaerosol Collection and Detection in Microfluidic Chips

Wang

Liu

et al. 2021

Anal. Chem.

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Bioaerosols containing pathogenic microorganisms have posed a great threat to human and animal health. Effective monitoring of bioaerosols containing pathogenic viruses and bacteria is of great significance to prevent and control infectious diseases. This Feature summarizes recent advances on bioaerosol collection and detection based on microfluidic chips. Besides, the challenges and trends for bioaerosol collection and detection were also discussed.

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Section: Bioaerosol Collectionmentioning

confidence: 99%

Recent Advances on Bioaerosol Collection and Detection in Microfluidic Chips

Wang

Liu

et al. 2021

Anal. Chem.

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“…The bioaerosols were first captured by the microfluidic herringbone chip and then irradiated with a 532 nm laser, resulting in a photothermal effect of spherical gold nanoparticles in the chip and thus the thermal lysis of bacteria. Finally, the extracted biomacromolecules were analyzed for quantitative detection of bioaerosols [ 94 ]. A thermoplasmonic-assisted dual-mode transducer was presented to detect the SARS-CoV-2 viruses in 30 min, where an amplification-free-based direct viral RNA detection and an amplification-based cyclic fluorescence probe cleavage detection were combined and demonstrated as a potential tool for fast clinical infection screening and real-time environmental monitoring [ 95 ].…”

Section: Pathogen Detectionmentioning

confidence: 99%

Challenges and Perspectives for Biosensing of Bioaerosol Containing Pathogenic Microorganisms

Lei

et al. 2021

Micromachines

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As an important route for disease transmission, bioaerosols have received increasing attention. In the past decades, many efforts were made to facilitate the development of bioaerosol monitoring; however, there are still some important challenges in bioaerosol collection and detection. Thus, recent advances in bioaerosol collection (such as sedimentation, filtration, centrifugation, impaction, impingement, and microfluidics) and detection methods (such as culture, molecular biological assay, and immunological assay) were summarized in this review. Besides, the important challenges and perspectives for bioaerosol biosensing were also discussed.

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“…This configuration requires blue LED illumination with additional beam-focusing components and relatively thick Au thin films. , Unlike Au thin films, geometry-controlled NPs facilitate the incorporation with a microfluidic chip, resulting in strong optical absorption at a specific wavelength for localized photothermal heating . For example, spherical Au NPs exploit visible laser beams to destroy the membrane of airborne and foodborne pathogens on the microfluidic chip. High aspect ratio Au nanorods use a near-infrared laser to induce more efficient on-chip photothermal lysis of bacteria.…”

Section: Introductionmentioning

confidence: 99%

Highly Efficient On-Chip Photothermal Cell Lysis for Nucleic Acid Extraction Using Localized Plasmonic Heating of Strongly Absorbing Au Nanoislands

Kang

Ahn

et al. 2023

ACS Appl. Mater. Interfaces

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Cell lysis serves as an essential role in the sample preparation for intracellular material extraction in lab-on-a-chip applications. However, recent microfluidic-based cell lysis chips still face several technical challenges such as reagent removal, complex design, and high fabrication cost. Here, we report highly efficient on-chip photothermal cell lysis for nucleic acid extraction using strongly absorbed plasmonic Au nanoislands (SAP-AuNIs). The highly efficient photothermal cell lysis chip (HEPCL chip) consists of a PDMS microfluidic chamber and densely distributed SAP-AuNIs with large diameters and small nanogaps, allowing for broad-spectrum light absorption. The SAP-AuNIs induce photothermal heat, resulting in a uniform temperature distribution within the chamber and rapidly reaching the target temperature for cell lysis within 30 s. Furthermore, the localized plasmonic heating of SAP-AuNIs expeditiously triggers phase transition and photoporation in the directly contacted lipid bilayer of the cell membrane, resulting in rapid and highly efficient cell lysis. The HEPCL chip successfully lysed 93% of PC9 cells at 90 °C for 90 s without nucleic acid degradation. This on-chip cell lysis offers a new sample preparation platform for integrated point-of-care molecular diagnostics.

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Continuous adsorption and photothermal lysis of airborne bacteria using a gold-nanoparticle-embedded-geometrically activated surface interaction (gold-GASI) chip.

Cited by 14 publications

References 20 publications

Recent Advances on Bioaerosol Collection and Detection in Microfluidic Chips

Recent Advances on Bioaerosol Collection and Detection in Microfluidic Chips

Challenges and Perspectives for Biosensing of Bioaerosol Containing Pathogenic Microorganisms

Highly Efficient On-Chip Photothermal Cell Lysis for Nucleic Acid Extraction Using Localized Plasmonic Heating of Strongly Absorbing Au Nanoislands

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