Continuous ambulatory peritoneal dialysis, ascitic and pleural body fluids evaluation with the Mindray <scp>BC</scp>‐6800 hematology analyzer

Fuster, Óscar; Andino, Belinda; Pardo, Andrea Vicente; Laíz, Begoña

doi:10.1002/jcla.22240

Cited by 8 publications

(14 citation statements)

References 21 publications

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“…16 More importantly, standard hematology analyzers capable of performing differential blood counts-which are permanently available at most hospitals-are reliable alternatives to manual counting to assess A-PMN counts. 26,27 We deem this relevant because in clinical routine, and especially in nontertiary centers, SBP diagnosis and treatment are often exclusively based on an A-LEUK count > 500 cells/ml. Furthermore, manual counting is often not available outside routine working hours on work days and on weekends.…”

Section: Discussionmentioning

confidence: 99%

“…It has recently been suggested that automatic assessment of A‐PMN cell counts with flow cytometry represents a more reliable technique for the diagnosis of SBP than traditional manual counting 16 . More importantly, standard hematology analyzers capable of performing differential blood counts—which are permanently available at most hospitals—are reliable alternatives to manual counting to assess A‐PMN counts 26 , 27 . We deem this relevant because in clinical routine, and especially in nontertiary centers, SBP diagnosis and treatment are often exclusively based on an A‐LEUK count > 500 cells/µl.…”

Section: Discussionmentioning

confidence: 99%

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Ascitic fluid polymorphic nuclear cell count impacts on outcome of cirrhotic patients with ascites

et al. 2019

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Background: Spontaneous bacterial peritonitis (SBP) is defined as an ascitic polymorphonuclear cell count (A-PMN) > 250 cells/ml. Objective: We aimed to investigate the prognostic value of ascitic fluid cell counts in patients without SBP. Patients and methods: A total of 178 patients were included and stratified by ascitic cell counts at index paracentesis: A-LEUK-low (<250/ml), A-LEUK-intermediate (250-500/ml) and A-LEUK-SBP (>500/ml) for leukocytes; A-PMN-low (<125/ml), A-PMN-intermediate (125-250/ml) and A-PMN-SBP (>250/ml) for PMN cells. Results: One-year mortality was comparable between group A-LEUK-SBP (53.9%) and patients with subclinical cell counts (34.5% for A-LEUK-low, 43.5% for A-LEUK-intermediate, log-rank p ¼ 0.547). However, we observed an increase in one-year mortality already in group A-PMN-intermediate with 75% and A-PMN-SBP with 80.9% (vs 40.5% for A-PMN-low, log-rank p ¼ 0.016). Importantly, increases of A-PMN cell counts between two paracenteses were associated with increased mortality: per 100 cells/ml increase of absolute A-PMN cell count: hazard ratio (HR): 1.03 (95% confidence interval (CI): 1.01-1.06), p ¼ 0.005; per 5% increase of relative PMN cell count: HR: 1.15 (95% CI: 1.06-1.26), p ¼ 0.001. Conclusion: Patients with PMN cell counts of 125-250/ml are at high risk for mortality, which was very similar to SBP patients with PMN cell counts >250/ml. This highlights the need for preventive strategies. The prognostic value of changes in relative ascitic PMN cell counts should be evaluated in future studies. Key summary1. Summary a. Spontaneous bacterial peritonitis (SBP) is the most frequent infection among patients with cirrhosis and ascites. b. SBP is defined by an ascitic polymorphonuclear (A-PMN) cell count higher than 250/ml in the absence of an intraabdominal source of infection. c. Development of bacteriascites was associated with an increase of relative A-PMN cell counts.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Ascitic fluid polymorphic nuclear cell count impacts on outcome of cirrhotic patients with ascites

et al. 2019

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“…El analizador BC-6800 es un analizador hematológico dotado de un módulo específico, no aprobado por la FDA, para el recuento de células en LCR, líquido sinovial y líquidos serosos; recientemente, ha sido también evaluada su utilidad en el líquido de diálisis peritoneal [30]. Dicho analizador proporciona el TNC-BF, WBC-BF, MN-BF y PMN-BF, e informa como parámetros de investigación el recuento de células de alta fluorescencia (HF-BF*), de neutrófilos (Neu-BF*) y eosinófilos (Eos-BF*).…”

Section: Analizadores Advia (Siemens Healthineers)unclassified

“…Sus principales ventajas son la disponibililidad de un material de control de calidad interno, el corto tiempo para el análisis (<3 minutos), el bajo volumen de muestra requerido (150 μL) y la posibilidad de procesar muestras sin pretratamiento previo. Estudios recientes han evaluado sus características analíticas (Tabla 1) y la transferibilidad de resultados respecto al método de referencia [25,[30][31][32][33][34][35], confirmando su utilidad en LCR [31] y en los líquidos pleural y ascítico [32]. Sin embargo, la evaluación cualitativa sistemática del escartegrama de diferenciación (DIFF) y del HF-BF* es esencial para la generación de algoritmos, como criterios para decidir la necesidad de la revisión microscópica [33].…”

Section: Analizadores Advia (Siemens Healthineers)unclassified

“…En líquido sinovial el recuento celular en el analizador BC-6800 puede reemplazar al análisis mediante microscopía óptica, siendo recomendable el pretratamiento del espécimen con hialuronidasa [35]. Aunque en líquido de diálisis peritoneal, el WBC-BF es transferible al recuento mediante microscopía óptica, la transferibilidad para el recuento diferencial no ha sido evaluada [30].…”

Section: Analizadores Advia (Siemens Healthineers)unclassified

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El recuento automatizado de células en líquidos biológicos: una revisión

Martín¹,

Queral²,

Frías³

et al. 2021

Advances in Laboratory Medicine / Avances en Medicina De Laboratorio

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Resumen El recuento de células en líquidos biológicos proporciona una información valiosa para el diagnóstico y tratamiento de diferentes patologías. El recuento en cámara y el estudio de la celularidad mediante microscopía óptica han sido consideradas tradicionalmente como método de referencia. Sin embargo, esta metodología implica un tiempo de respuesta del laboratorio elevado, carece de la reproducibilidad adecuada y requiere de personal experto. El avance tecnológico ha permitido el desarrollo de módulos de análisis específicos para los líquidos biológicos, incorporados en analizadores de hematología y de orinas, que permiten la automatización del recuento celular y han sido rápidamente incorporados a la práctica asistencial de los laboratorios En la actualidad diferentes analizadores están disponibles para ofrecer soluciones de automatización en el recuento de células en líquidos biológicos. Sin embargo, el empleo de dichos analizadores no está exento de limitaciones y su utilización requiere de un profundo conocimiento por los especialistas de la Medicina de Laboratorio. En esta revisión, se describen las principales tecnologías para la automatización del recuento celular en líquidos biológicos, el significado de los parámetros informados por los analizadores, sus principales características analíticas, así como el papel de la microscopía óptica en un contexto de utilización creciente de estas tecnologías.

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Clinical application of an algorithm to screen for malignant cells in body fluids using an automated hematology analyzer

Cho

Kim

Cho

et al. 2022

Int J Lab Hematology

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In clinical laboratories, meaningful results are derived through cell counts based on the morphology of cells in a specimen. Analyzing the differential white blood cell (WBC) count of body fluid (BF) provides important information for clinical judgment, such as infection, inflammatory disease, and malignancy. 1 Detecting malignant cells is a major problem in clinical laboratories. 2 Conventionally, cytological examination (microscopic method) has been the gold standard for detecting malignant cells in BFs. [3][4][5][6][7][8] However, conventional

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Continuous ambulatory peritoneal dialysis, ascitic and pleural body fluids evaluation with the Mindray BC‐6800 hematology analyzer

Cited by 8 publications

References 21 publications

Ascitic fluid polymorphic nuclear cell count impacts on outcome of cirrhotic patients with ascites

Ascitic fluid polymorphic nuclear cell count impacts on outcome of cirrhotic patients with ascites

El recuento automatizado de células en líquidos biológicos: una revisión

Clinical application of an algorithm to screen for malignant cells in body fluids using an automated hematology analyzer

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