Continuous and autonomous-flow separation of laccase enzyme utilizing functionalized aqueous two-phase system with computer vision control

Sharma, Vikas; Joo, Jeong-Un; Mottafegh, Amirreza; Kim, Dong-Pyo

doi:10.1016/j.biortech.2024.130888

Bioresource Technology

2024

DOI: 10.1016/j.biortech.2024.130888

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Continuous and autonomous-flow separation of laccase enzyme utilizing functionalized aqueous two-phase system with computer vision control

Vikas Sharma,

Jeong-Un Joo,

Amirreza Mottafegh

et al.

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Cited by 2 publications

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Effect of flow patterns on recovery of invertases from Saccharomyces cerevisiae using polyethylene glycol/magnesium sulfate system in microchannels

Carvalho,

Costa,

de Oliveira

et al. 2024

Food and Bioproducts Processing

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Effect of flow patterns on recovery of invertases from Saccharomyces cerevisiae using polyethylene glycol/magnesium sulfate system in microchannels

Carvalho,

Costa,

de Oliveira

et al. 2024

Food and Bioproducts Processing

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Advancements in Aqueous Two-Phase Systems for Enzyme Extraction, Purification, and Biotransformation

Bekavac,

Benković,

Jurina

et al. 2024

Molecules

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In recent years, the increasing need for energy conservation and environmental protection has driven industries to explore more efficient and sustainable processes. Liquid–liquid extraction (LLE) is a common method used in various sectors for separating components of liquid mixtures. However, the traditional use of toxic solvents poses significant health and environmental risks, prompting the shift toward green solvents. This review deals with the principles, applications, and advantages of aqueous two-phase systems (ATPS) as an alternative to conventional LLE. ATPS, which typically utilize water and nontoxic components, offer significant benefits such as high purity and single-step biomolecule extraction. This paper explores the thermodynamic principles of ATPS, factors influencing enzyme partitioning, and recent advancements in the field. Specific emphasis is placed on the use of ATPS for enzyme extraction, showcasing its potential in improving yields and purity while minimizing environmental impact. The review also highlights the role of ionic liquids and deep eutectic solvents in enhancing the efficiency of ATPS, making them viable for industrial applications. The discussion extends to the challenges of integrating ATPS into biotransformation processes, including enzyme stability and process optimization. Through comprehensive analysis, this paper aims to provide insights into the future prospects of ATPS in sustainable industrial practices and biotechnological applications.

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Continuous and autonomous-flow separation of laccase enzyme utilizing functionalized aqueous two-phase system with computer vision control

Cited by 2 publications

References 45 publications

Effect of flow patterns on recovery of invertases from Saccharomyces cerevisiae using polyethylene glycol/magnesium sulfate system in microchannels

Effect of flow patterns on recovery of invertases from Saccharomyces cerevisiae using polyethylene glycol/magnesium sulfate system in microchannels

Advancements in Aqueous Two-Phase Systems for Enzyme Extraction, Purification, and Biotransformation

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