Continuous and High Cell Density Cultivation of Escherichia coli W3110(pE901) Harboring Human Interferon Alpha A Gene

Ye, Qin; F, Kang; Lu, Shican; Tao, Jianmin; Zhang, Siliang; Yu, Jianguo

doi:10.1007/978-4-431-68180-9_60

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“…Increasing the productivity is therefore possible by increasing the specific productivity or the biomass concentration or both. Maximum biomass concentrations are usually reached by addition of complex components such as yeast extract, tryptone, and casamino acids (Olsson and Hahn-Hägerdal, 1995;Chung et al, 1992;Ye et al, 1992;Hasio et al, 1992). More recently considerable effort has been spent on cultivating E. coli to high cell densities on defined medium (Weuster-Botz et al, 1995;Korz et al, 1995;Riesenberg et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Growth characteristics ofEscherichia coliHB101[pGEc47] on defined medium

Rothen

Sauer

Sonnleitner

1998

Biotechnol. Bioeng.

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This paper shows that differences in growth behavior of Escherichia coli strain HB101 and strain HB101[pGEc47] can be related to yeast extract‐enriched medium rather than plasmid properties. An optimal medium for growth of E. coli HB101[pGEc47] was designed based on the individual yield coefficients for specific medium components (NH4+ 6 g g−1, PO43− 14 g g−1, SO42− 50 g g−1). The yield coefficient for l‐leucine depends on the glucose content of the medium (20 g g−1 for 3% glucose, 40 g g−1 for 1% glucose) and the yield coefficient for l‐proline depends on the cultivation mode (20 g g−1 for batch cultivation, 44 g g−1 for continuous cultivation). Growth on defined medium after medium optimization is as rapid as on complex medium (0.42–0.45 h−1). The critical dilution rate (DR) in the defined medium above which undesired production of acetic acid occurs is in the range of 0.23–0.26 h−1. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58:92–100, 1998.

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