Continuous aqueous two-phase extraction of human antibodies using a packed column

Rosa, P.A.J.; Azevedo, Ana M.; Sommerfeld, Sven D.; Bäcker, W.; Aires-Barros, M. Raquel

doi:10.1016/j.jchromb.2011.11.034

Cited by 61 publications

(43 citation statements)

References 18 publications

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“…Similar to the counter-current extraction results presented by Rosa et al [22], an increase in the distribution coefficient of the impurities was observed. The side component distribution coefficient at stage 1 was 0.95, while at stage 10 the distribution coefficient was 6.5.…”

Section: Mixer-settler Batterysupporting

confidence: 89%

“…For the process and system pre- sented in the current study, the purification was about 10 % higher than reported in the previous study. The impurities removal in this study was 86-95 %, comparing favorably to the previously published removal of 85 % for the column extraction of Rosa et al [22].…”

Section: Multi-stage Washsupporting

confidence: 88%

“…In comparison to the only available results by Rosa et al [22], the yield and purity had the same order of magnitude. For the process and system pre- sented in the current study, the purification was about 10 % higher than reported in the previous study.…”

Section: Multi-stage Washsupporting

confidence: 56%

“…First approaches using a multi-stage ATPE for the purification of mAb have been reported by Rosa et al [21,22]. For a cell culture harvest synthetically mixed with an immunoglobulin G (IgG) antibody in a NaCl-containing ATPS, 62 % purity at 73 % yield was obtained for the cross-current process, and 50 % total contaminant removal at 85 % yield was obtained for the counter-current process.…”

Section: Introductionmentioning

confidence: 99%

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Multi‐Stage Aqueous Two‐Phase Extraction for the Purification of Monoclonal Antibodies

et al. 2014

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Using monoclonal antibodies in a cell culture harvest as an example for complex biomolecules, a mini-plant-scale aqueous two-phase purification process was studied. The results of this study indicate that antibodies can be concentrated and purified in a single extraction step employing a small phase ratio. Following the extraction step, a multi-stage wash-extraction for further purification was investigated. Starting at a test tube-scale cross-current wash, the process was advanced towards a continuous counter-current mixer-settler and column wash process. It was shown that a test tube cross-current extraction operation can predict the multi-stage purity reasonably well. The hydrodynamic process performance for the multi-stage wash column was evaluated and related to the separation performance.

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Section: Mixer-settler Batterysupporting

confidence: 89%

Section: Multi-stage Washsupporting

confidence: 88%

Section: Multi-stage Washsupporting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

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Multi‐Stage Aqueous Two‐Phase Extraction for the Purification of Monoclonal Antibodies

et al. 2014

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“…However, in spite of all its advantages, this unit operation has not gained wide industrial recognition in the field of biotechnology owing to its poor solubility and possible protein denaturation in organic solvents (20); therefore, its use in biotechnology has been limited to the recovery of low molecular weight products, such as antibiotics and organic acids from fermentation broths (5). ATPSs are more suitable as a downstream processing technology of bioproducts, particularly for continuous operation (7,21,22). Chromatography Techniques.…”

Section: Atps Technology Compared With Other Techniquesmentioning

confidence: 99%

Aqueous Two‐Phase Systems (ATPSs) for Extraction and Purification of Biomolecules and Bioparticles

Marques

Molino

Mazzola

et al. 2013

Encyclopedia of Industrial Biotechnology

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The study of biomolecules and bioparticles has achieved great advances, thus increasing the productivity with upstream improvements. Nowadays, downstream processes are the bottleneck in the production of some biopharmaceuticals, a change from the previous years' scene. Current purification platforms will reach their physical limits at some point, creating the necessity for new approaches. This article reviews an alternative method for the extraction and purification of biomolecules/bioparticles denominated as aqueous two‐phase systems (ATPSs) . Biocompatibility and readiness to scale up are some of the characteristics of ATPSs and we also discuss its application in the biotechnology field.

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Continuous Downstream Processing for Production of Biotech Therapeutics

Rathore

Kateja

Agarwal

2017

Continuous Biomanufacturing ‐ Innovative Technologies and Methods

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Continuous aqueous two-phase extraction of human antibodies using a packed column

Cited by 61 publications

References 18 publications

Multi‐Stage Aqueous Two‐Phase Extraction for the Purification of Monoclonal Antibodies

Multi‐Stage Aqueous Two‐Phase Extraction for the Purification of Monoclonal Antibodies

Aqueous Two‐Phase Systems (ATPSs) for Extraction and Purification of Biomolecules and Bioparticles

Continuous Downstream Processing for Production of Biotech Therapeutics

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