Continuous cultivation of Penicillium chrysogenum. Growth on glucose and penicillin production

Christiansen, Lasse Engbo; Henriksen, Claus Maxel; Nielsen, Jens; Egel-Mitani, Michi

doi:10.1016/0168-1656(95)00056-v

Cited by 52 publications

(46 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(6) is the most popular one to describe the relationship between the specific substrate uptake rate and other cell culture parameters (Pirt, 1965). It has been widely used to study the maximum cell yield on substrate and maintenance coefficient (Abbott and Clamen, 1973;Christensen et al, 1995;Kayser et al, 2005). The maintenance coefficient indicates the amount of substrate needed per unit cell mass per hour to maintain cell in an active and proper physiological condition.…”

Section: Resultsmentioning

confidence: 99%

Kinetics of continuous cultivation of the oleaginous yeast Rhodosporidium toruloides

Shen

Gong

Yang

et al. 2013

Journal of Biotechnology

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Kinetics of continuous cultivation of the oleaginous yeast Rhodosporidium toruloides

Shen

Gong

Yang

et al. 2013

Journal of Biotechnology

View full text Add to dashboard Cite

“…In agreement, our results supported the production increase in the high-producing strains being mediated by the other mechanisms rather than titre-enhancing mutations in the cis-regulatory elements of the penicillin biosynthesis genes. One of these possible mechanisms is the amplification of these genes that is quite common in P. chrysogenum production strains (Barredo et al 1989b;Smith et al 1989Smith et al , 1990Christensen et al 1995). This was examined in the both high-producing strains, NMU2/40 and B14.…”

Section: Discussionmentioning

confidence: 99%

Sequence analysis and gene amplification study of the penicillin biosynthesis gene cluster from different strains of Penicillium chrysogenum

et al. 2010

View full text Add to dashboard Cite

Industrial strains of Penicillium chrysogenum possess many genomic changes leading to higher levels of penicillin. In this work several production and wild-type strains of Penicillium chrysogenum were used in comparative nucleotide sequence analysis of the biosynthesis cluster. The alignments confirmed sequence conservation not only in promoter regions of the biosynthesis genes but also throughout the entire 44.7-kbp genomic fragment comprising the whole biosynthesis cluster with 15.5-kbp and 13.1-kbp flanking regions. As another titre-enhancing mechanism we subsequently examined gene dosage in two production strains introduced here, NMU2/40 and B14. Quantitative real-time PCR and Southern blot analysis showed the amplification of the biosynthesis genes in both these strains. Through the real-time PCR method the exact copy number was estimated for each of the pcbAB, pcbC and penDE genes. The equal pool of all three genes per genome was confirmed for the both production strains indicating that in these strains the entire penicillin cluster has been amplified as an intact element. Penicillium chrysogenum NMU2/40 was found to carry four copies of the cluster, while six copies were estimated for B14. This also proves the contribution of the additional titre-enhancing mechanisms in both strains, since the industrial data referred much higher production of these strains compared with the single copy reference strain NRRL 1951.

show abstract

“…The results are summarized in Table I, together with previously reported values using high-yielding penicillinproducing strains (Christensen et al, 1995;van Gulik et al, 2001).…”

Section: Growth Stoichiometry On Glucosementioning

confidence: 59%

“…After some continuous cultivation time, white colonies appeared on the plates. No intermediate phenotypes (light-green colonies), as found by Christensen et al (1995), were observed. The white and green colonies were counted and the percentage of green colonies was determined.…”

Section: Instability Of Strain Under Glucose-limited Conditionsmentioning

confidence: 79%

“…Extensive studies of several penicillin-producing strains of Penicillium chrysogenum have been carried out, and the growth stoichiometry of these strains on various carbon sources such as glucose, ethanol, and acetate has been determined (Christensen et al, 1995;van Gulik et al, 2000). In addition to detailed characterization of the metabolism of penicillin-producing strains, some studies have focused on the relationship between morphological development and productivity (McIntyre et al, 2001;Paul et al, 1994;Paul and Thomas, 1996).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Continuous cultivations of a Penicillium chrysogenum strain expressing the expandase gene from Streptomyces clavuligerus: Growth yields and morphological characterization

Robin

Lettier

McIntyre

et al. 2003

Biotech & Bioengineering

Self Cite

View full text Add to dashboard Cite

The growth stoichiometry of a Penicillium chrysogenum strain expressing the expandase gene from Streptomyces clavuligerus was determined in glucose-limited chemostat cultivations using a chemically defined medium. This strain produces adipoyl-7-aminodeacetoxycephalosporanic acid (ad-7-ADCA) when it is fed with adipic acid. The biomass yield and maintenance coefficients for the strain were similar to those found for penicillin-producing strains of Penicillium chrysogenum. The maximum specific growth rate in the chemostat was found to be 0.11 h(-1). Metabolic degradation of adipate was found to take place in significant amounts only at dilution rates below 0.03 h(-1). After three to five residence times, adipate degradation and ad-7-ADCA production disappeared, and this allowed determination of the biomass yield coefficient on adipate. The morphology was measured at different dilution rates and the mean total hyphal length and mean number of tips both increased with an increase in dilution rate from 0.015 to 0.065 h(-1). Both variables decreased when the dilution rate was increased above 0.065 h(-1). A correlation between mean total hyphal length and productivity of ad-7-ADCA was found.

show abstract

Continuous cultivation of Penicillium chrysogenum. Growth on glucose and penicillin production

Cited by 52 publications

References 19 publications

Kinetics of continuous cultivation of the oleaginous yeast Rhodosporidium toruloides

Kinetics of continuous cultivation of the oleaginous yeast Rhodosporidium toruloides

Sequence analysis and gene amplification study of the penicillin biosynthesis gene cluster from different strains of Penicillium chrysogenum

Continuous cultivations of a Penicillium chrysogenum strain expressing the expandase gene from Streptomyces clavuligerus: Growth yields and morphological characterization

Contact Info

Product

Resources

About