Continuous culture of human faecal bacteria as an in vitro model for the colonic microflora

Bearne, C.A.; Mallett, A.K.; Rowland, Ian; Brennan-Craddock, W.E.

doi:10.1016/0887-2333(90)90111-6

Cited by 18 publications

(6 citation statements)

References 8 publications

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“…Both in vitro and in vivo models of the human gut have been developed. The in vitro models include batch cultures of faeces (Wang and Gibson 1993) and continuous-flow cultures simulating the microecology of the intestine (Bearne et al 1990). However, even complex in vitro models may not completely simulate the effects of biological parameters provided by the digestive and gut immune systems.…”

Section: Discussionmentioning

confidence: 99%

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Improvement of human faecal flora-associated mouse model for evaluation of the functional foods

et al. 2004

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Aims: Animal models are required for evaluation of the functional foods such as pro/prebiotics exerting effects through the metabolism of the intestinal microflora. The object of this study was to establish new human floraassociated mice reflecting the environment of the human intestinal tract. Methods and Results: We inoculated a human faecal suspension into segmented filamentous bacteria (SFB) monoassociated mice as a model system. In both human flora (HF) and SFB-associated mouse (HF-SFB mouse), intestinal characteristics such as the composition of intraepithelial lymphocytes, the expression of major histocompatibility complex (MHC) class II molecules and the number of immunoglobulin A-producing cells in the mucosa was closer to those of conventionally reared mice than was case with human flora-associated mice (HF mice) lacking SFB. Several predominant bacterial groups except lactobacilli in human flora were found in faeces of HF-SFB mice. Lactobacilli established small populations in the gut of HF-SFB mice when administered before inoculation with the human flora. Faecal enzymatic activities and organic acid concentration of HF-SFB mice proportionally reflected those of the donor subject. Conclusion: We established a new human flora-associated mouse (HF-SFB mouse), in which intestinal characteristics are normally developed and their major microbial composition reflect the human. Significance and Impact of the Study: HF-SFB mice are a valuable model for studying pro/prebiotic effects on the human intestine.

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Section: Discussionmentioning

confidence: 99%

“…Both in vitro and in vivo models of the human gut have been developed. The in vitro models include batch cultures of faeces (Wang and Gibson 1993) and continuous‐flow cultures simulating the microecology of the intestine (Bearne et al. 1990).…”

Section: Discussionmentioning

confidence: 99%

Improvement of human faecal flora-associated mouse model for evaluation of the functional foods

et al. 2004

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“…tabolism (3,5,13,15,22,23,25). We showed that the growth of bifidobacteria and acetate production were enhanced, especially one day after incubation, when supplemented with sterilized P. freudenreichii culture into a 2-stage ACCS (21).…”

Section: Effect Of Bgs On Gutmentioning

confidence: 99%

A Novel Bifidogenic Growth Stimulator Produced by Propionibacterium freudenreichii

Kaneko

1999

Bioscience Microflora

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A novel bifidogenic growth stimulator (BGS) was present in the cell-free filtrate and in methanol extract fraction of the starter (Propionibacterium freudenreichii) cells for the manufacture of Swiss-type cheese. After purification of the BGS isolated from the lyophilized cells, the mass (217.037) was determined by high-resolution electron impact mass spectrometry (MS) and liquid chromatography-MS spectra. Various experimental analyses indicated that the chemical structure of the BGS was 2-amino-3-carboxy-1,4-naphthoquinone (ACNQ). We examined the activity of the P. freudenreichii ET culture containing the BGS on a gut bacterial composition using an anaerobic continuous culture system and found that the BGS seems to enhance the selective utilization of oligosaccharides by bifidobacteria. Furthermore, a placebo-controlled study of the effects of BGS on fecal flora and stool frequency was carried out in healthy human subjects. A drink with the sterilized ET-3 culture was administered once a day for 7 days. Bifidobacterium percentage in the fecal flora and stool frequency were significantly increased by administration of the P. freudenreichii culture. The ACNQ exhibited growth stimulation of bifidobacteria at an extremely low concentration and enhanced the activities of NADH oxidase and NADH peroxidase in bifidobacteria. It was revealed that ACNQ works as a good electron acceptor of NAD(P)H diaphorase. The ACNQred was easily auto-oxidized and also acted as a better electron donor of NAD(P)H peroxidase. These ACNQ-mediated reactions seem to play roles in NAD(P)+-regeneration processes and seem to be responsible for the growth stimulation of bifidobacteria.

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“…Continuous in¯ow of growth media, and out¯ow of biomass, enable a diverse micro¯ora to be maintained in a steady state (Rumney & Rowland, 1992). Models of the human gut of varying degrees of complexity have now been developed (Miller & Wolin, 1981;Allison et al 1989;Bearne et al 1990;Molly et al 1994;Minekus & Havenaar, 1996;. Such models maintain a micro¯ora similar in many ways to that of the human gut in gross species composition and activities.…”

Section: In Vitro Modelsmentioning

confidence: 99%

Perspectives on the role of the human gut microbiota and its modulation by pro- and prebiotics

et al. 2000

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One of the most topical areas of human nutrition is the role of the gut in health and disease. Specifically, this involves interactions between the resident microbiota and dietary ingredients that support their activities. Currently, it is accepted that the gut microflora contains pathogenic, benign and beneficial components. Some microbially induced disease states such as acute gastroenteritis and pseudomembranous colitis have a defined aetiological agent(s). Speculation on the role of microbiota components in disorders such as irritable bowel syndrome, bowel cancer, neonatal necrotising enterocolitis and ulcerative colitis are less well defined, but many studies are convincing. It is evident that the gut microflora composition can be altered through diet. Because of their perceived health-promoting status, bifidobacteria and lactobacilli are the commonest targets. Probiotics involve the use of live micro-organisms in food; prebiotics are carbohydrates selectively metabolized by desirable moieties of the indigenous flora; synbiotics combine the two approaches. Dietary intervention of the human gut microbiota is feasible and has been proven as efficacious in volunteer trials. The health bonuses of such approaches offer the potential to manage many gut disorders prophylactically. However, it is imperative that the best methodologies available are applied to this area of nutritional sciences. This will undoubtedly involve a genomic application to the research and is already under way through molecular tracking of microbiota changes to diet in controlled human trials.

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Continuous culture of human faecal bacteria as an in vitro model for the colonic microflora

Cited by 18 publications

References 8 publications

Improvement of human faecal flora-associated mouse model for evaluation of the functional foods

Improvement of human faecal flora-associated mouse model for evaluation of the functional foods

A Novel Bifidogenic Growth Stimulator Produced by Propionibacterium freudenreichii

Perspectives on the role of the human gut microbiota and its modulation by pro- and prebiotics

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