Continuous degradation of phenol(s) by Pseudomonas putida P8 entrapped in polyacrylamidehydrazide

Bettmann, Heinz; Rehm, H. J.

doi:10.1007/bf00252778

Cited by 46 publications

(9 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some reports are available on microbial degradation of phenol W e i n rtul. 1979; Beltmann and Rehm 1985;Ehrhardt and Rehm 1985;Sokol 1988;Lallai and Mura 1989) but meagre data are available on the use of microorganisms for treatment of phenol containing industrial waste eflluents (Anselmo and Novais 1984;Atlow etal. 1984;Jeter 1985;Sarnaik 1991,1995;Brenner etal.…”

Section: Introductionmentioning

confidence: 99%

Bioremediation of phenol by alkaliphilic bacteria isolated from alkaline lake of Lonar, India

Kanekar

Sarnaik

Kelkar

1998

Journal of Applied Microbiology

View full text Add to dashboard Cite

SUMMARY Phenol is an industrially important compound which has a wide range of applications. Being highly soluble in water, it appears as the major pollutant in waste waters arising from both phenol manufacturing and from industrial units that utilise phenol. Because of its toxicity, bioremediation of phenol is necessary. Since some of the phenol‐bearing industrial waste waters are alkaline in nature, use of alkaliphilic bacteria for bioremediation of phenol was investigated. Alkaliphilic bacteria were isolated from sediments of an alkaline lake in Lonar, Dist. Buldhana, Maharashtra State, India, by phenol enrichment at pH 10·0 and phenol concentration of 500 mg/l. The lake (lat. 19°58′45”, long. 76°34’) is known to be a unique inland saline lake in Asia. It has a circular periphery and diameter of 2 km around the top of the banks and 1·2 km at the bottom. The lake has a high saline level (∼ 2649 mg/l sodium chloride) and a high level of alkalinity (∼ 2605 mg/l calcium carbonate). Alkaliphilic strains of Arthrobacter spp., Bacillus cereus, Citrobacter freundii, Micrococcus agilis and Pseudomonas putida biovar B were capable of removing phenol from waste waters arising from industries manufacturing methyl violet (using phenol as one of the major raw materials) and cumene‐phenol. The waste waters from both these units were alkaline in nature (pH ∼ 9·95–10·1) and had a high phenol content (368–660 mg/l). The alkaliphilic bacteria being studied removed 100% of the phenol from the industrial waste waters within 48 h of incubation under shake culture conditions and at an ambient temperature of 28 ± 2 °C. Bioremediation of phenol by alkaliphilic strains of Arthrobacter spp., B. cereus, C. freundii and M. agilis seems to be the first report.

show abstract

Section: Introductionmentioning

confidence: 99%

Bioremediation of phenol by alkaliphilic bacteria isolated from alkaline lake of Lonar, India

Kanekar

Sarnaik

Kelkar

1998

Journal of Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…A few other polymers were also used for immobilizing microorganisms for phenol removal. PAAH-immobilized P. putida P8 in a fluidized bed reactor, at a dilution rate of 0.067, degraded phenol at a rate of 7.2 g l -1 day -1 (300 mg l -1 h -1 ) under sterile condition and 3.12 g l -1 day -1 (130 mg l -1 h -1 ) under non-sterile condition from an initial 2 g l -1 phenol (Bettmann and Rehm 1985). A mixed culture of Acinetobacter sp.…”

Section: Discussionmentioning

confidence: 99%

“…Ca-alginate has been used as the gel matrix in several studies for encapsulating strains of bacteria, yeasts, and mycelial fungi (Abd-El-Haleem et al 2003;Bandhyopadhyay et al 1999;Bettmann and Rehm 1984;Chung et al 2003;Dursun and Tepe 2005;Hannaford and Kuek 1999;Kapoor et al 1998;Karigar et al 2006;Keweloh et al 1989;Lakhwala et al 1992;Mordocco et al 1999;Santos et al 2003). Other polymers which were occasionally used for immobilizing phenol-degrading microorganisms were agar, polyacrylamide (PAA), polyacrylamide hydrazide (PAAH), polyvinyl alcohol (PVA), polysulfone hollow fiber membrane, and modified polyacrylonitrile membrane (Bettmann and Rehm 1985;Chen et al 2002;Juárez-Ramírez et al 2001;Karigar et al 2006;Liu et al 2009;Loh et al 2000;Yordanova et al 2009). All the phenol-degrading strains exhibited improved ability, to varying degrees, in the immobilized systems than as freely suspended cells.…”

Section: Introductionmentioning

confidence: 99%

Enhanced degradation of phenol by Pseudomonas sp. CP4 entrapped in agar and calcium alginate beads in batch and continuous processes

Ahamad

Kunhi

2010

Biodegradation

View full text Add to dashboard Cite

Phenol is one of the major toxic pollutants in the wastes generated by a number of industries and needs to be eliminated before their discharge. Although microbial degradation is a preferred method of waste treatment for phenol removal, the general inability of the degrading strains to tolerate higher substrate concentrations has been a bottleneck. Immobilization of the microorganism in suitable matrices has been shown to circumvent this problem to some extent. In this study, cells of Pseudomonas sp. CP4, a laboratory isolate that degrades phenol, cresols, and other aromatics, were immobilized by entrapment in Ca-alginate and agar gel beads, separately and their performance in a fluidized bed bioreactor was compared. In batch runs, with an aeration rate of 1 vol(-1) vol(-1) min(-1), at 30°C and pH 7.0 ± 0.2, agar-encapsulated cells degraded up to 3000 mg l(-1) of phenol as compared to 1500 mg l(-1) by Ca-alginate-entrapped cells whereas free cells could tolerate only 1000 mg l(-1). In a continuous process with Ca-alginate entrapped cells a degradation rate of 200 mg phenol l(-1) h(-1) was obtained while agar-entrapped cells were far superior and could withstand and degrade up to 4000 mg phenol l(-1) in the feed with a maximum degradation rate of 400 mg phenol l(-1) h(-1). The results indicate a clear possibility of development of an efficient treatment technology for phenol containing waste waters with the agar-entrapped bacterial strain, Pseudomonas sp. CP4.

show abstract

“…Earlier studies examined the application of immobilized, pure cultures and reactor systems for synthetic phenolic wastewater treatment [6][7][8][9][10]. However, the application of immobilized pure culture cells for wastewater treatment may not be practical because of mixed substrate and culture characteristics in many wastewater treatment processes.…”

Section: Introductionmentioning

confidence: 99%

Packed entrapped mixed microbial cell process for removal of phenol and its compounds

Yang

See

1991

Journal of Environmental Science and Health . Part A: Environme

View full text Add to dashboard Cite

show abstract

Continuous degradation of phenol(s) by Pseudomonas putida P8 entrapped in polyacrylamidehydrazide

Cited by 46 publications

References 10 publications

Bioremediation of phenol by alkaliphilic bacteria isolated from alkaline lake of Lonar, India

Bioremediation of phenol by alkaliphilic bacteria isolated from alkaline lake of Lonar, India

Enhanced degradation of phenol by Pseudomonas sp. CP4 entrapped in agar and calcium alginate beads in batch and continuous processes

Packed entrapped mixed microbial cell process for removal of phenol and its compounds

Contact Info

Product

Resources

About