Continuous Electrocardiogram Monitoring in Zebrafish with Prolonged Mild Anesthesia

Le, Tai; Zhang, Jimmy; Xia, Xing; Xu, Xiaolei; Clark, Isaac; Schmiess-Heine, Lauren; Nguyen, Anh H.; Lau, Michael; Cao, Hung

doi:10.1109/embc44109.2020.9175576

Cited by 5 publications

(2 citation statements)

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“…Four syringes contain low dose Tricaine solution, continuously providing the solution to the fish through the tubing system to reduce the aggressiveness and activity of zebrafish but keep them awake. Four valves adjust the solution's flow rate within a range of 5.5 -6 ml/min [53]. Housing apparatuses and sensors are improved from the previous work [53].…”

Section: Design Of the Zebra IImentioning

confidence: 99%

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A Novel Art of Electrocardiogram Assessment in Zebrafish for Cardiovascular Disease Studies and Drug Screening

Zhang

et al. 2021

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The zebrafish (Dario rerio) has proven to be an excellent animal model for biological research owing to its small size, low cost for maintenance, short generation time, amenable genetics, and optical transparency. Zebrafish have been extensively used in cardiovascular studies in which mutant lines with cardiovascular defects were introduced and analyzed. Despite the small size, technological advances have paved the way to effectively assess cardiac functions of zebrafish. Here, we present a novel art for long-term simultaneous monitoring and analysis of electrocardiogram (ECG) in multiple zebrafish with controlled environment. The system helps minimize the effect of anesthetic drug and temperature to cardiac rhythm side effects as well as save time and efforts by 40-50 fold compared with the conventional approach. We further employed the system to study the Na+ sensitivity in the development of sinus arrest in Tg(SCN5A-D1275N) fish, a study model of the sick sinus syndrome, as well as the relationship between this variant and drug administration. The novel ECG system developed in this study holds promise to greatly accelerate other cardiovascular studies and drug screening using zebrafish.

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Section: Design Of the Zebra IImentioning

confidence: 99%

“…Four valves adjust the solution's flow rate within a range of 5.5 -6 ml/min [53]. Housing apparatuses and sensors are improved from the previous work [53]. Specifically, multiple small housings are made of polydimethylsiloxane (PDMS), providing comfort to the fish and thus minimizing unwanted artifacts.…”

Section: Design Of the Zebra IImentioning

confidence: 99%