Continuous flow microfluidic device for cell separation, cell lysis and DNA purification

Chen, Xing; Cui, Dafu; Liu, Changchun; Li, Hui; Chen, Jian

doi:10.1016/j.aca.2006.11.057

Cited by 170 publications

(130 citation statements)

References 19 publications

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“…Combining microfluidic systems with different readout techniques has produced powerful tools in the emerging area of the on-chip controlled handling and monitoring of biological and synthetic microparticles and nanoparticles, with especially great impact on biotechnology and environmental topics, such as hydrodynamic forces [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20], optical methods [21,22], electric fields and dielectrophoresis [23][24][25][26][27] or acoustic fields , either isolated or combined with other fields.…”

Section: Introductionmentioning

confidence: 99%

Optimizing Polymer Lab-on-Chip Platforms for Ultrasonic Manipulation: Influence of the Substrate

González

Tijero²,

Martin

et al. 2015

Micromachines

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Abstract:The choice of substrate material in a chip that combines ultrasound with microfluidics for handling biological and synthetic microparticles can have a profound effect on the performance of the device. This is due to the high surface-to-volume ratio that exists within such small structures and acquires particular relevance in polymer-based resonators with 3D standing waves. This paper presents three chips developed to perform particle flow-through separation by ultrasound based on a polymeric SU-8 layer containing channelization over three different substrates: Polymethyl methacrylate (PMMA); Pyrex; and a cracked PMMA composite-like structure. Through direct observations of polystyrene microbeads inside the channel, the three checked chips exhibit their potential as disposable continuous concentration devices with different spatial pressure patterns at frequencies of resonance close to 1 Mhz. Chips with Pyrex and cracked PMMA substrates show restrictions on the number of pressure nodes established in the channel associated with the inhibition of 3D modes in the solid structure. The glass-substrate chip presents some advantages associated with lower energy requirements to collect particles. According to the OPEN ACCESSMicromachines 2015, 6 575 results, the use of polymer-based chips with rigid substrates can be advantageous for applications that require short treatment times (clinical tests handling human samples) and low-cost fabrication.

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Section: Introductionmentioning

confidence: 99%

Optimizing Polymer Lab-on-Chip Platforms for Ultrasonic Manipulation: Influence of the Substrate

González

Tijero²,

Martin

et al. 2015

Micromachines

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“…Chen et al and Sethu et al developed crossflow filtration systems with one main channel in the centre and two side channels in a parallel orientation. Micropillar [17] and microweir [18] arrays are located at the interfaces between the main and lateral channels, enabling cross flows. With the optimized flow dynamics, WBC-enriched and depleted blood fractions can be harvested from the outlets of main channel and lateral channels, respectively (Figure 1iii).…”

Section: Filtrationmentioning

confidence: 99%

On-chip sample preparations for Point-of-Care cellular analysis of blood

Zhang¹,

Terstappen²,

Beck³

2017

Point-of-Care Diagnostics - New Progresses and Perspectives

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“…10,[14][15][16] In the oscillating flow PCR system the temperatures in the denaturing, annealing, and extension zones were kept constant over time while the sample was moving through these individual zones ͑Fig. 1͒.…”

Section: A Chip Design and Fabricationmentioning

confidence: 99%

Amplification of SPPS150 and Salmonella typhi DNA with a high throughput oscillating flow polymerase chain reaction device

Sugumar

Ravichandran

Ismail³

et al. 2010

Biomicrofluidics

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In this paper, a novel oscillating flow polymerase chain reaction ͑PCR͒ device was designed and fabricated to amplify SPPS150 and salmonella typhi. In this new design, the samples are shuttled ͑oscillating flow͒ inside a microfluidic chip to three different temperature zones required for DNA amplification. The amplification cycle time has markedly been reduced as the reagent volume used was only about 25% of that used in conventional PCRs. Bubble formation and adsorption issues commonly associated to chip based PCR were also eliminated. Based on the performance evaluated, it is demonstrated that this oscillating flow PCR has the advantages of both the stationary chamber and continuous flow PCR devices.

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Continuous flow microfluidic device for cell separation, cell lysis and DNA purification

Cited by 170 publications

References 19 publications

Optimizing Polymer Lab-on-Chip Platforms for Ultrasonic Manipulation: Influence of the Substrate

Optimizing Polymer Lab-on-Chip Platforms for Ultrasonic Manipulation: Influence of the Substrate

On-chip sample preparations for Point-of-Care cellular analysis of blood

Amplification of SPPS150 and Salmonella typhi DNA with a high throughput oscillating flow polymerase chain reaction device

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