Continuous fractionation of plasma proteins HSA and HIgG using cascade ultrafiltration systems

Mayani, Mukesh; Mohanty, Kaustubha; Filipe, Carlos D. M.; Ghosh, Raja

doi:10.1016/j.seppur.2009.10.002

Cited by 18 publications

(5 citation statements)

References 28 publications

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“…These proof‐of‐concept experiments show that uncrosslinked polymeric membranes can potentially enable purification of bulk solvent/solvent mixtures. However, practical issues such as the effect of stage cut, process upsets and fluctuations, contaminants, and so on, must still be considered …”

Section: Resultsmentioning

confidence: 99%

Torlon® hollow fiber membranes for organic solvent reverse osmosis separation of complex aromatic hydrocarbon mixtures

Jang

Johnson

et al. 2019

AIChE Journal

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Defect‐free polyamide‐imide (Torlon®) hollow fiber membranes were fabricated to investigate the potential for polymer‐based organic solvent reverse osmosis (OSRO) separations. The quality of the membranes was assessed by gas permeation, and the membranes were found to be defect‐free. Low molecular weight cut‐offs of ~180 g/mol were obtained using a complex mixture of aromatic hydrocarbons relevant to refinery separations. We demonstrate bulk OSRO‐type separations of 80/20 (mol%) mixtures of toluene and 1,3,5‐triisopropylbenzene (TIPB, 204 g/mol). At an upstream pressure of 80–90 bar, we find that the permeate concentration was approximately 98.5–99.0 mol% toluene and that the TIPB rejection coefficient was approximately 90% in the permeate. We observed low solvent permeances of 0.01 L/m2 hr bar, which can be attributed to the low OSRO driving forces and the low permeability of Torlon®. The membranes were found to provide stable performance up to pressures of 95 bar and temperatures of 60°C.

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Section: Resultsmentioning

confidence: 99%

Torlon® hollow fiber membranes for organic solvent reverse osmosis separation of complex aromatic hydrocarbon mixtures

Jang

Johnson

et al. 2019

AIChE Journal

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“…Membrane cascades have been widely studied for applications such as desalination, water purification and the fractionation of solutes in mixture (Abatemarco et al, 1999;Caus et al, 2009;Ebara et al, 1978;Mayani et al, 2009;Mellal et al, 2007). The design and operation of membrane cascades can be complex due to the many combinations of design and operation variables.…”

Section: Introductionmentioning

confidence: 99%

Iterative peptide synthesis in membrane cascades: Untangling operational decisions

Chen

Sharifzadeh

Shah

et al. 2018

Computers & Chemical Engineering

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Membrane enhanced peptide synthesis (MEPS) combines liquid-phase synthesis with membrane filtration, avoiding time-consuming separation steps such as precipitation and drying. Although performing MEPS in a multi-stage cascade is advantageous over a single-stage configuration in terms of overall yield, this is offset by the complex combination of operational variables such as the diavolume and recycle ratio in each diafiltration process. This research aims to tackle this problem using dynamic process simulation. The results suggest that the two-stage membrane cascade improves the overall yield of MEPS significantly from 72.2% to 95.3%, although more washing is required to remove impurities as the second-stage membrane retains impurities together with the anchored peptide. This clearly indicates a link between process configuration and operation. While the case study is based on the comparison of single-stage and two-stage MEPS, the results are transferable to other biopolymers such as oligonucleotides, and more complex system configurations (e.g. three-stage MEPS).

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“…In addition, the proposed process configurations are typically too simple and unsuitable for carrying out in an effective way the fractionation, particularly in situations where recovery of very pure fractions is desired. Nevertheless, membrane cascades have demonstrated their usefulness as effective configurations to coordinate several separation stages in order to overcome the constraints of limited separation processes based on membranes, because the overall selectivity can be significantly improved. − This type of configuration has been widely used for the separation and fractionation of proteins and other biological molecules by membrane technologies, − but further efforts to progress in the complete design of industrial-scale installations based on this advanced configuration are still pending.…”

Section: Introductionmentioning

confidence: 99%

In Silico Evaluation of Ultrafiltration and Nanofiltration Membrane Cascades for Continuous Fractionation of Protein Hydrolysate from Tuna Processing Byproduct

Abejón

Garea

et al. 2016

Ind. Eng. Chem. Res.

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The present work proposes the design of cascades that integrate ultrafiltration (UF) and nanofiltration (NF) membranes to separate the different protein fractions from the protein hydrolysate obtained after hydrolysis of tuna byproducts. Experimental data (permeate flux and rejection of protein fractions under different applied pressures) previously obtained and published by this research group were fitted to empirical models, which were the basis for a process simulation model. High recovery rates (0.9) in the UF stages implied high process yields by reduced desired fraction losses, while similar recovery rates in the NF stages were required for high product purity. However, the applied pressures were not so influential over the performance of the system. Optimization problems were solved to identify the optimal design and operation conditions to maximize the product purity or the process yield. Maximal purity of the preferred 1−4 kDa fraction (49.3% from 19.0% in feed stream) obtained by the configuration with 3 UF stages and another 3 NF stages implied 2 and 5 bar pressures applied in the UF and NF stages, respectively, while 0.9 was the optimal recovery rate value for all the stages. These maximal purity conditions resulted in 62.6% process yield, defined as the percentage of the 1−4 kDa fraction in the feed stream recovered in the product stream. In addition, multiobjective optimization of the process was also carried out to obtain the Pareto graphs that represent the counterbalance between maximal yields and purities.

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Continuous fractionation of plasma proteins HSA and HIgG using cascade ultrafiltration systems

Cited by 18 publications

References 28 publications

Torlon® hollow fiber membranes for organic solvent reverse osmosis separation of complex aromatic hydrocarbon mixtures

Torlon® hollow fiber membranes for organic solvent reverse osmosis separation of complex aromatic hydrocarbon mixtures

Iterative peptide synthesis in membrane cascades: Untangling operational decisions

In Silico Evaluation of Ultrafiltration and Nanofiltration Membrane Cascades for Continuous Fractionation of Protein Hydrolysate from Tuna Processing Byproduct

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