Continuous glucose monitoring and control in a rotating wall perfused bioreactor

Abstract

A glucose control system consisting of a single in-line glucose sensor, concentrated glucose solution, and computer hardware and software were developed. The system was applied to continuously control glucose concentrations of a perfusion medium in a rotating wall perfused vessel (RWPV) bioreactor culturing BHK-21 cells. The custom-made glucose sensor was based on a hydrogen peroxide electrode. The sensor continuously and accurately measured the glucose concentration of GTSF-2 medium in the RWPV bioreactor during cell culture. Three sets of two-point calibrations were applied to the glucose sensor during the 55-day cell culture. The system first controlled the glucose concentration in perfusing medium between 4.2 and 5.6 mM for 36 days and then at different glucose levels for 19 days. A stock solution with a high glucose concentration (266 mM) was used as the glucose injection solution. The standard error of prediction (SEP) for glucose measurement by the sensor, compared to measurement by the Beckman glucose analyzer, was +/-0.4 mM for 55 days.

Computational Study of Culture Conditions and Nutrient Supply in Cartilage Tissue Engineering

“…In general, following the time course of the concentrations in the bulk medium is important, for the interpretation of measurements and the prediction of concentrations within the construct. Furthermore, combining such (on-line) measurements with relatively simple numerical models can prove useful for future bioreactor control applications (55,62). The type of numerical modeling employed in the current study is suited to quickly provide first order directions in the design of bioreactor setups and culture protocols.…”

Self-reporting Scaffolds for 3-Dimensional Cell Culture

“…This would allow assessment of nutrient supply to deeper regions within the structure and determine whether metabolic waste was being removed effectively 10,11 . Systems that attempt to address the issue of invasiveness generally involve the use of a perfusion chamber that passes culture medium through both the culture vessel and to external sensors to monitor pH, oxygen and glucose 12 . There is increasing interest in developing sensors that can be directly integrated into the culture vessel that do not require removal of an aliquot for sampling and as such would provide in situ monitoring.…”