Continuous Hydrolysis of Olive Oil by Lipase in Microporous Hydrophobic Hollow Fiber Bioreactor

Hoq, Mohammad Mozammel; Koike, Miyuki; Yamané, Tsuneo; Shimizu, Sakayu

doi:10.1080/00021369.1985.10867235

Cited by 10 publications

(8 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The application of membrane reactors in lipid processing is still in its infancy, but many reports have focused on using membranes as the separation media (Snape and Nakajima, 1996). Membrane reactors for enzyme-catalyzed lipid reaction systems have been mainly used in the hydrolysis of oils and fats (Hoq et al, 1985;Prond et al, 1988;Taylor and Craig, 1991;Garcia et al, 1992;Goto et al, 1992;Cuperus et al, 1993;Prazeres et al, 1993) and the esterification between alcohol and fatty acids (van der Padt et al, 1990;1992). In few cases of lipase-catalyzed interesterification, membranes were used to retain the lipases when the reaction mixture was circulated (Basheer et al, 1995c) or as the carrier for lipase immobilization (Balcao and Malcata, 1998).…”

Section: Membrane Reactorsmentioning

confidence: 99%

Modification of Oils and Fats by Lipase‐Catalyzed Interesterification: Aspects of Process Engineering

2000

Enzymes in Lipid Modification

View full text Add to dashboard Cite

Section: Membrane Reactorsmentioning

confidence: 99%

Modification of Oils and Fats by Lipase‐Catalyzed Interesterification: Aspects of Process Engineering

2000

Enzymes in Lipid Modification

View full text Add to dashboard Cite

“…The applications usually improve the process of production, such as shortening the process, decreasing the temperature, leading the reaction to a specific direction, etc., and have many advantages over other separation techniques (2). Most investigations of membrane applications in lipid separation have been focused on the recovery of solvent from micella, separation in degumming, refining and bleaching, condensate return, catalyst recovery (2)(3)(4), hydrolyses of oils and fats (5)(6)(7)(8), or the syntheses of acylglycerols in two-phase membrane reactors (9). The direct separation of free fatty acids (FFA) from triacylglycerols was also investigated in a membrane reactor (10) and a review on enzymatic membrane reactors has been published (11).…”

mentioning

confidence: 99%

“…then C b α t [5] where t is the permeation time. If C a = 100% and C b is not negligible, the flux is:…”

mentioning

confidence: 99%

Production of structured lipids by lipase‐catalyzed interesterification in a flat membrane reactor

Balchen

Jonsson

et al. 2000

J Americ Oil Chem Soc

View full text Add to dashboard Cite

The application of membrane technology to the enzymatic production of specific structured lipids has been investigated in this work. Membrane screening was carried out in a membrane diffusion cell. Twenty-six flat membranes of different materials were tested using rapeseed oil and capric acid. The suitable membranes were selected in terms of higher fatty acid and lower rapeseed oil permeation rates. The stability of membranes and the effect of fatty acid chain length on effluent fluxes were also investigated. Reaction experiments were carried out in a membrane reactor between medium-chain triacylglycerols and n-3 polyunsaturated fatty acids (PUFA) from fish oil. Lipozyme IM was used as the biocatalyst. The incorporation of PUFA into medium-chain triacylglycerols was increased by about 15% in a PUFA 90-h reaction by simultaneous separation of the released medium-chain fatty acids, compared to no separation under the same reaction conditions. It has thus clearly been demonstrated that membrane-assisted separation improved the incorporation of acyl donors into oils beyond the reaction equilibrium defined by the original substrate concentration. MATERIALS AND METHODSSubstrates. MCT, containing 60.0 mol% caprylic acid and 40.0 mol% capric acid by analysis, was purchased from Grü-nau GmbH (Illertissen, Germany). Refined rapeseed oil was a donation from Aarhus Oliefabrik A/S (Aarhus, Denmark). The fatty acid composition of the rapeseed oil (mol%) was: C 16:0 , 6.0; C 16:1 , 0.2; C 18:0 , 1.6; C 18:1n-9 , 58.8; C 18:2n-6 , 21.9; C 18:3n-3 , 10.0; and C 20:1n-9 , 0.6. Capric acid was purchased from Henkel Kimianika Sdn. Bhd.; purity: 99.6 mol%, Selangor, Malaysia. Oleic acid (87% purity) was purchased from Riedel-de Haen (Seelze, Germany). An eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) concentrate (mixture of 35% EPA and 25% DHA) and a DHA product (85% purity) were donated by Pronova Biocare A.S. (Sandefjord, Norway). Lipozyme IM (Novo Nordisk A/S, Bagsvaerd, Denmark) consists of an sn-1,3-specific lipase from Rhizomucor miehei, immobilized on a macroporous ion exchange resin (water content 3.3%). All solvents and reagents for analyses were of analytical grade.Membranes. G-10 and Desal-5 were donated by Desal (Euro/Africa Office, Hauptstrasse, Switzerland). Other membranes were donated by Dow Denmark Separation Systems, (Nakskov, Denmark). The details of the membranes are listed in Table 1. GR, FS, and all microfiltration membranes were obtained in the wet state, saturated with water or an aqueous solution, and the membranes were soaked in ethanol before use. The rest of the membranes were tested in their dry state.Membrane reactor. The reactor is depicted in Figure 1. A 1036 X. XU ET AL. FIG. 2.The typical relationship between the free fatty acid (FFA) content (wt%) in rapeseed oil (Chamber B) and permeation time. Conditions: membrane, ETNA10A (Dow Denmark Separation Systems, Nakskov, Denmark); temperature, 60°C; and skin layer side of membrane facing Chamber B (rapeseed oil side).

show abstract

“…The enzymatic hydrolysis of lipids is an interesting process as it could help overcome some of the drawbacks of a high-temperature process. Recently, membrane reactors with immobilized lipase have been reported for a successful olive oil (2)(3)(4) or sunflower oil (5,6), hydrolysis. A microporous membrane is the enzyme's carrier and separates the bulk phases.…”

mentioning

confidence: 99%

Polymeric membranes for lipase immobilization

Rucka

Turkiewicz

żuk

1990

J Americ Oil Chem Soc

View full text Add to dashboard Cite

Lipase triacylglycerol acylhydrolase, (E.C. 3.1.1.3) is an enzyme that is fully active on aggregated substrates and practically inactive on monodisperse systems. A lipase immobilized on polymeric membranes has been applied for sunflower oil hydrolysis. The influence of membrane properties on enzyme activity is studied. Membranes made of poly(vinyl chloride), collagen, cellulose acetate and polytetrafluoroethylene were used for adsorption of lipase. The porosity and hydrophobicity of membranes did not influence the lipase activity. The difference of the work of adhesion for the water/membrane system and oil/membrane system reflected the activity data, while work of adhesion for water or oil (done separately) did not. For oil hydrolysis to occur on the membrane surface, accessibility of two liquid phases is important, and the lower the difference of work of adhesion between water and oil, the greater the activity of immobilized lipase.

show abstract

Continuous Hydrolysis of Olive Oil by Lipase in Microporous Hydrophobic Hollow Fiber Bioreactor

Cited by 10 publications

References 3 publications

Modification of Oils and Fats by Lipase‐Catalyzed Interesterification: Aspects of Process Engineering

Modification of Oils and Fats by Lipase‐Catalyzed Interesterification: Aspects of Process Engineering

Production of structured lipids by lipase‐catalyzed interesterification in a flat membrane reactor

Polymeric membranes for lipase immobilization

Contact Info

Product

Resources

About