Continuous <i>in vitro</i> culture of <i>Babesia divergens</i>
 in a 

serum-free medium

Grande, N.; Précigout, Éric; Ancelin, Marie-Laure; Moubri, K.; Carcy, B.; Lemesre, Jean-Loup; Vial, Henri; Gorenflot, A.

doi:10.1017/s0031182097008937

Cited by 32 publications

(19 citation statements)

References 7 publications

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“…Various species of Babesia have previously been propagated in medium without serum, for example, B. occultans and Babesia sp. (Zweygarth et al 1995b) and B. divergens (Grande et al 1997). The two stocks of B. caballi studied in this work, however, were the only ones that produced a higher PPE than did control cultures propagated in serum-containing medium.…”

Section: Discussionmentioning

confidence: 63%

Continuous in vitro cultivation of Babesia caballi in serum-free medium

Zweygarth

Niekerk

Waal

1999

Parasitology Research

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Experiments were undertaken to develop a serum-free medium for the in vitro cultivation of Babesia caballi, a tick-borne hemoprotozoan parasite, one of the causative agents of equine piroplasmosis. A modified HL-1 medium supplemented with horse serum, L-glutamine, antibiotics, and hypoxanthine was used. B. caballi organisms were continuously cultivated at 37 degrees C in microaerophilous stationary-phase culture in a humidified atmosphere containing 5% CO2 in air before exposure to serum-free culture conditions. For serum-free propagation, lipid-rich bovine serum albumin (LR-BSA), alone or with chemically defined lipids (CDL), were added instead of serum. Media containing LR-BSA alone or LR-BSA and CDL in various amounts supported the in vitro propagation of B. caballi. Growth was maintained for more than 6 months. The growth rates obtained in serum-free media were similar to those previously obtained in traditional serum-containing medium.

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Section: Discussionmentioning

confidence: 63%

Continuous in vitro cultivation of Babesia caballi in serum-free medium

Zweygarth

Niekerk

Waal

1999

Parasitology Research

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“…Since Vayrynen and Tuomi first reported successful long-term in vitro cultivation in bovine erythrocytes (182), the parasite has been successfully maintained in human (63,67,141), rat (8), and sheep (29,192) erythrocytes. Although B. divergens was also able to invade horse and donkey erythrocytes (Fig.…”

Section: Host Specificity and Parasite Morphologymentioning

confidence: 99%

Babesia divergens , a Bovine Blood Parasite of Veterinary and Zoonotic Importance

et al. 2003

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Babesia divergens is an intraerythrocytic protozoan parasite, transmitted by the tick Ixodes ricinus, and is the main agent of bovine babesiosis in Europe. It is not only a cause of significant loss to the cattle industry; it can also infect immunocompromised humans, causing medical emergencies characterized by rapid fulmination and parasitemias that may exceed 70%. The current emphasis in Europe on sustainable agriculture and extensification is likely to lead to an increase in vector tick populations with increased risk of infection. Despite the veterinary and zoonotic importance of this parasite, relatively little research has been carried out on B. divergens, and many questions regarding the parasite's epidemiology and the host's response remain unanswered. A better understanding of the species' biology and host-parasite interactions may lead to improved control mechanisms and new trends in vaccine and antibabesial drug development. This review provides the first comprehensive summary of B. divergens biology, including its morphology, life cycle, and host specificity, and the current state of knowledge of both human and bovine infections

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“…15,2002 CULTIVATION OF BABESIA 369 replication. Grande et al (16) postulated that the parasites' need for phospholipid associated with de novo membrane formation is the factor that limited growth in host erythrocytes and that this need can be satisfied only by an exogenous source of phospholipid and a carrier such as albumin. They note, however, that PPE was not restored to its normal level of 40 to 50%, indicating that serum provided other still-undetermined components needed for optimal growth of the parasite.…”

Section: B Divergensmentioning

confidence: 99%

Cultivation ofBabesiaandBabesia-Like Blood Parasites: Agents of an Emerging Zoonotic Disease

Schuster

2002

Clin Microbiol Rev

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Babesia and its close relatives are members of a group of organisms called piroplasms, a name which comes from their pear-shaped outlines. Long associated with blood diseases of cattle and other mammals, members of the genus Babesia have been recognized since the 1950s as infectious agents in humans. Species of this protozoan blood parasite that have routinely been isolated from mice (B. microti) or cattle (B. divergens) have also been isolated from humans. In addition to these familiar species, new isolates that resist being placed in existing taxonomic categories are the basis for rethinking their phylogenetic relationships based on sequencing data. The parasite represents a threat to the safety of the blood supply in that blood from asymptomatic humans can transmit Babesia to blood recipients. Such transmissions have occurred. The development of methods for cultivation of these organisms represents a significant opportunity to study their biology and disease potential. In addition, in vitro cultivation has provided a basis for studying immune responses of mammals to these infectious agents, with the hope of ultimately producing attenuated strains that could be used for immunizing of cattle and, perhaps, humans who live in areas of endemicity. The microaerophilous stationary phase culture technique, which uses a tissue culture medium base supplemented with appropriate serum and erythrocytes, has made it possible to obtain large numbers of parasitized erythrocytes for studying the biology of this parasite

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Continuous in vitro culture of Babesia divergens in a serum-free medium

Cited by 32 publications

References 7 publications

Continuous in vitro cultivation of Babesia caballi in serum-free medium

Continuous in vitro cultivation of Babesia caballi in serum-free medium

Babesia divergens , a Bovine Blood Parasite of Veterinary and Zoonotic Importance

Cultivation ofBabesiaandBabesia-Like Blood Parasites: Agents of an Emerging Zoonotic Disease

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