Continuous-mode acclimation and operation of lignocellulosic sulfate-reducing bioreactors for enhanced metal immobilization from acidic mining-influenced water

Miranda, Evelyn M; Severson, Carli; Reep, Jeffrey K.; Hood, Daniel; Hansen, Shane; Santisteban, Leonard; Hamdan, Nasser; Delgado, Anca G.

doi:10.1016/j.jhazmat.2021.128054

Cited by 10 publications

(32 citation statements)

References 94 publications

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“…The method details and calibration were described previously. 68 detection limit was 0.3 μM (0.03 mg L −1 ). Lactate, formate, acetate, and propionate were measured using a high-performance liquid chromatograph (HPLC, Shimadzu LC-20AT) equipped with an Aminex HPX-87H column (Biorad, Hercules, CA) as previously described.…”

Section: ■ Materials and Methodsmentioning

confidence: 98%

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Decoupling Fe⁰ Application and Bioaugmentation in Space and Time Enables Microbial Reductive Dechlorination of Trichloroethene to Ethene: Evidence from Soil Columns

Rangan

Rao

Robles

et al. 2023

Environ. Sci. Technol.

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Fe0 is a powerful chemical reductant with applications for remediation of chlorinated solvents, including tetrachloroethene and trichloroethene. Its utilization efficiency at contaminated sites is limited because most of the electrons from Fe0 are channeled to the reduction of water to H2 rather than to the reduction of the contaminants. Coupling Fe0 with H2-utilizing organohalide-respiring bacteria (i.e., Dehalococcoides mccartyi) could enhance trichloroethene conversion to ethene while maximizing Fe0 utilization efficiency. Columns packed with aquifer materials have been used to assess the efficacy of a treatment combining in space and time Fe0 and aD. mccartyi-containing culture (bioaugmentation). To date, most column studies documented only partial conversion of the solvents to chlorinated byproducts, calling into question the feasibility of Fe0 to promote complete microbial reductive dechlorination. In this study, we decoupled the application of Fe0 in space and time from the addition of organic substrates andD. mccartyi-containing cultures. We used a column containing soil and Fe0 (at 15 g L–1 in porewater) and fed it with groundwater as a proxy for an upstream Fe0 injection zone dominated by abiotic reactions and biostimulated/bioaugmented soil columns (Bio-columns) as proxies for downstream microbiological zones. Results showed that Bio-columns receiving reduced groundwater from the Fe0-column supported microbial reductive dechlorination, yielding up to 98% trichloroethene conversion to ethene. The microbial community in the Bio-columns established with Fe0-reduced groundwater also sustained trichloroethene reduction to ethene (up to 100%) when challenged with aerobic groundwater. This study supports a conceptual model where decoupling the application of Fe0 and biostimulation/bioaugmentation in space and/or time could augment microbial trichloroethene reductive dechlorination, particularly under oxic conditions.

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Section: ■ Materials and Methodsmentioning

confidence: 98%

“…The ion chromatography method was based on EPA Method 9056A. The method details and calibration were described previously . SO 4 2– detection limit was 0.3 μM (0.03 mg L –1 ).…”

Section: Methodsmentioning

confidence: 99%

Decoupling Fe⁰ Application and Bioaugmentation in Space and Time Enables Microbial Reductive Dechlorination of Trichloroethene to Ethene: Evidence from Soil Columns

Rangan

Rao

Robles

et al. 2023

Environ. Sci. Technol.

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“…The slag was sieved, retaining particles in the 9–20 mm diameter range. Spent brewing grains and sugarcane bagasse were used as the lignocellulosic substrates in this study because of their documented potential in stimulating SO 4 2– reduction in SRBRs. ,, Spent brewing grains (after the mashing process) were obtained from SanTan Brewery in Chandler, Arizona, USA, and were transported to Arizona State University on the same day. Sugarcane bagasse was provided by Cajun Sugar Company in New Iberia, Louisiana.…”

Section: Methodsmentioning

confidence: 99%

“…Synthetic MIW (referred henceforth as MIW) was prepared as described in Miranda et al 30 The composition of the MIW from this study, including the type and concentration of metal(loid)s and concentration of SO 4 2− , was based on the characteristics of an actual MIW from a legacy hard rock mine (see Table S1 The SRBR stage consisted of two sets of SRBRs packed with either spent brewing grains or sugarcane bagasse. The slag reactor and SRBRs were constructed from clear, schedule 40 PVC pipes (L = 116 cm, ID = 10 cm, V = 9.1 L) and were mounted using strut channel clamps to a shelf.…”

Section: Materials and Preparation Of Synthetic Miwmentioning

confidence: 99%

“…The enrichment process and the composition of the microbial community of these cultures were reported in a previous study. 30 MIW from the settling tank entered the SRBRs at the top. The SRBRs were operated directly in continuous mode (no batch operation) as previously done 30 to acclimate the microbial community to MIW.…”

Section: Sulfate-reducing Biochemical Reactor (Srbr)mentioning

confidence: 99%

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High Efficacy Two-Stage Metal Treatment Incorporating Basic Oxygen Furnace Slag and Microbiological Sulfate Reduction

Miranda,

McLaughlin,

Reep

et al. 2024

ACS EST Eng.

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Lignocellulosic sulfate-reducing biochemical reactors (SRBRs) can be implemented as passive treatment for mining-influenced water (MIW) mitigating the potentially deleterious effects of MIW acidic pH, and high concentrations of metal(loid)s and SO 4 2−. In this study, a novel two-stage treatment for MIW was designed, where basic oxygen furnace slag (slag stage) and microbial SO 4 2− reduction (SRBR stage) were incorporated in series. The SRBRs contained spent brewing grains or sugarcane bagasse as sources of lignocellulose. The slag reactor removed >99% of the metal(loid) concentration present in the MIW (130 ± 40 mg L −1 ) and increased MIW pH from 2.6 ± 0.2 to 12 ± 0.3. The alkaline effluent pH of the slag reactor was mitigated by remixing slag effluent with acidic MIW before SRBR treatment. The SRBR stage removed the bulk of SO 4 2− from MIW, additional metal(loid)s, and yielded a circumneutral effluent pH. Cadmium, copper, and zinc showed high removal rates in SRBRs (≥96%) and likely precipitated as sulfide minerals. The microbial communities developed in SRBRs were enriched in hydrolytic, fermentative, and sulfatereducing taxa. However, the SRBRs developed distinct community compositions due to the different lignocellulose sources employed. Overall, this study underscores the potential of a two-stage treatment employing steel slag and SRBRs for full-scale implementation at mining sites.

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Enhancing the fermentability of brewer’s spent grains to short- and medium-chain carboxylates through dilute alkaline pretreatment

Henry,

Isenborghs,

Walhain

et al. 2024

Biomass Conv. Bioref.

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Continuous-mode acclimation and operation of lignocellulosic sulfate-reducing bioreactors for enhanced metal immobilization from acidic mining-influenced water

Cited by 10 publications

References 94 publications

Decoupling Fe⁰ Application and Bioaugmentation in Space and Time Enables Microbial Reductive Dechlorination of Trichloroethene to Ethene: Evidence from Soil Columns

Decoupling Fe⁰ Application and Bioaugmentation in Space and Time Enables Microbial Reductive Dechlorination of Trichloroethene to Ethene: Evidence from Soil Columns

High Efficacy Two-Stage Metal Treatment Incorporating Basic Oxygen Furnace Slag and Microbiological Sulfate Reduction

Enhancing the fermentability of brewer’s spent grains to short- and medium-chain carboxylates through dilute alkaline pretreatment

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Continuous-mode acclimation and operation of lignocellulosic sulfate-reducing bioreactors for enhanced metal immobilization from acidic mining-influenced water

Cited by 10 publications

References 94 publications

Decoupling Fe0 Application and Bioaugmentation in Space and Time Enables Microbial Reductive Dechlorination of Trichloroethene to Ethene: Evidence from Soil Columns

Decoupling Fe0 Application and Bioaugmentation in Space and Time Enables Microbial Reductive Dechlorination of Trichloroethene to Ethene: Evidence from Soil Columns

High Efficacy Two-Stage Metal Treatment Incorporating Basic Oxygen Furnace Slag and Microbiological Sulfate Reduction

Enhancing the fermentability of brewer’s spent grains to short- and medium-chain carboxylates through dilute alkaline pretreatment

Contact Info

Product

Resources

About

Decoupling Fe⁰ Application and Bioaugmentation in Space and Time Enables Microbial Reductive Dechlorination of Trichloroethene to Ethene: Evidence from Soil Columns

Decoupling Fe⁰ Application and Bioaugmentation in Space and Time Enables Microbial Reductive Dechlorination of Trichloroethene to Ethene: Evidence from Soil Columns