Continuous monitoring of cell transfection efficiency with micropatterned substrates

Azuaje-Hualde, Enrique; Rosique, Melania; Calatayud-Sánchez, Alba; Benito‐Lopez, Fernando; Pancorbo, Marian M. de; Basabe‐Desmonts, Lourdes

doi:10.1002/bit.27783

Cited by 2 publications

(2 citation statements)

References 41 publications

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“…under controlled cell-cell contact) would benefit from these advantages to evolve into fully formed devices with manufacturing and commercialization prospects [4,28,29].…”

Section: Patterning Of Cells On the Hydrophilic Pmma Surfacesmentioning

confidence: 99%

“…These include cell sorting, especially for circulating cells and corpuscles, the assessment of cellular interactions with substrates and biochemical compounds, as well as evaluating cellular responses to chemical stimuli. Our previous works on the use of cell patterns for the optical monitoring of cell behaviors (monitoring of cell adhesion and biosensing of their integrin profile, cell death under cytotoxic treatments and cell transfection under controlled cell-cell contact) would benefit from these advantages to evolve into fully formed devices with manufacturing and commercialization prospects [4,28,29].…”

Section: Maintenance Of the Cell Patterns Under Flow Conditionsmentioning

confidence: 99%

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Cell Patterning Technology on Polymethyl Methacrylate through Controlled Physicochemical and Biochemical Functionalization

Azuaje-Hualde,

Komen,

Alonso-Cabrera

et al. 2023

Biosensors

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In recent years, innovative cell-based biosensing systems have been developed, showing impact in healthcare and life science research. Now, there is a need to design mass-production processes to enable their commercialization and reach society. However, current protocols for their fabrication employ materials that are not optimal for industrial production, and their preparation requires several chemical coating steps, resulting in cumbersome protocols. We have developed a simplified two-step method for generating controlled cell patterns on PMMA, a durable and transparent material frequently employed in the mass manufacturing of microfluidic devices. It involves air plasma and microcontact printing. This approach allows the formation of well-defined cell arrays on PMMA without the need for blocking agents to define the patterns. Patterns of various adherent cell types in dozens of individual cell cultures, allowing the regulation of cell–material and cell–cell interactions, were developed. These cell patterns were integrated into a microfluidic device, and their viability for more than 20 h under controlled flow conditions was demonstrated. This work demonstrated the potential to adapt polymeric cytophobic materials to simple fabrication protocols of cell-based microsystems, leveraging the possibilities for commercialization.

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“…under controlled cell-cell contact) would benefit from these advantages to evolve into fully formed devices with manufacturing and commercialization prospects [4,28,29].…”

Section: Patterning Of Cells On the Hydrophilic Pmma Surfacesmentioning

confidence: 99%

Section: Maintenance Of the Cell Patterns Under Flow Conditionsmentioning

confidence: 99%

Cell Patterning Technology on Polymethyl Methacrylate through Controlled Physicochemical and Biochemical Functionalization

Azuaje-Hualde,

Komen,

Alonso-Cabrera

et al. 2023

Biosensors

Self Cite

View full text Add to dashboard Cite

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CellStudio: a Modular, Tunable and Accessible Platform for Analysis of Growth Factors Secretions in Cell Cultures

Azuaje-Hualde,

Lartitegui-Meneses,

Alonso-Cabrera

et al. 2024

Preprint

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Traditional cell culture methods face significant limitations in monitoring cell secretions with spatial and temporal precision. Advanced microsystems incorporating biosensors have been developed to address these challenges, but they tend to lack versatility, and their complexity, along with the requirement for specialized equipment, limits their broader adoption. CellStudio offers an innovative, user-friendly solution that exploits the Printing and Vacuum Lithography combined with bead-based assays to create modular and tunable cell patterns surrounded by biosensors. This platform allows for high-resolution, spatially resolved analysis of secreted proteins, such as VEGF and FGF-2, while being easily implementable in standard laboratory settings. CellStudio's design is compatible with conventional laboratory equipment, facilitating its integration into existing workflows without the need for extensive training or specialized tools. Validation experiments using mesenchymal stem cells (MSCs) and HeLa cells demonstrated that CellStudio can detect small secretion levels from small cell clusters with high sensitivity as well as analyze diffusion profiles, remarking the possibilities for studying cell behavior. By offering a standardized, cost-effective approach to detailed cellular analysis, CellStudio significantly enhances the capabilities of traditional cell culture techniques, with broad applications across biological and biomedical research.

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Continuous monitoring of cell transfection efficiency with micropatterned substrates

Cited by 2 publications

References 41 publications

Cell Patterning Technology on Polymethyl Methacrylate through Controlled Physicochemical and Biochemical Functionalization

Cell Patterning Technology on Polymethyl Methacrylate through Controlled Physicochemical and Biochemical Functionalization

CellStudio: a Modular, Tunable and Accessible Platform for Analysis of Growth Factors Secretions in Cell Cultures

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