Continuous monitoring of chemical signals in plants under stress

Coatsworth, Philip; Gonzalez‐Macia, Laura; Collins, Alexander Silva Pinto; Bozkurt, Tolga O.; Güder, Firat

doi:10.1038/s41570-022-00443-0

Cited by 38 publications

(27 citation statements)

References 158 publications

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“…Although, in this work, we mostly studied the rates of uptake of ions, with the existing TETRIS setup consisting of printed pH, EIS and H2O2 sensors, biochemical signaling and feedback loops associated with, but not limited to, plant hormones, pesticides, pathogenic or beneficial microorganisms can be studied. 12 Because TETRIS is modular and customizable, the number of printed chemical sensors integrated into the system can be expanded to include sensors specific to certain ions or molecules. 35 Furthermore, TETRIS can be used as an artificial rhizosphere to study root microbiome to investigate plant-microbe interactions, and potentially allow creations of digital twins of these complex systems.…”

Section: Discussionmentioning

confidence: 99%

“…The multiplexed nature of TETRIS allows simultaneous sensing of different analytes in the same sample, including concentrations of salts (via EIS), H2O2 and pH, where we chose these analytes due to their relevance as signals in plant physiological processes and stress response (Figure 1b-e). 12 The sensing module, holding a single sensor (or multiple sensors for multiplexed measurements), was placed into the chamber, consisting of a silicone base and transparent acrylic lid (Figure 2a). A reservoir of water was created in the silicone base to ensure that a level of nearly 100% relative humidity was maintained to prevent evaporation of water from the root environment and sensor, and therefore prevent erroneous measurements during the test (see supplementary information All experiments were performed with the plants grown on discs of chromatography paper (Whatman 1).…”

Section: General Experimental Setup Of Tetrismentioning

confidence: 99%

“…11 Electrochemical sensors for continuous-time phenotyping of whole plants are emerging as an alternative to optical methods. [12][13][14][15][16] Insertable electrochemical sensors (sensors that are inserted into plant tissues, such as stems or leaves) are typically formed either of conductive electrodes or microfabricated field-effect transistors and have been used to measure the internal chemical environment of plant tissues. Insertable sensors can detect bursts of H2O2 resulting from pathogenic and UV light stresses in leaf tissue, enabling characterization of responses to these stressors.…”

Section: Introductionmentioning

confidence: 99%

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Time-Resolved Chemical Phenotyping of Whole Plant Roots with Printed Electrochemical Sensors and Machine Learning

Coatsworth

Cotur

Naik

et al. 2023

Preprint

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Plants are non-equilibrium systems consisting of time-dependent biological processes. Phenotyping of chemical responses, however, is typically performed using plant tissues, which behave differently to whole plants, in one-off measurements. Single point measurements cannot capture the information rich time-resolved changes in chemical signals in plants associated with nutrient uptake, immunity or growth. In this work, we report a high-throughput, modular, real-time chemical phenotyping platform for continuous monitoring of chemical signals in the often-neglected root environment of whole plants: TETRIS (Time-resolvedElectrochemicalTechnology for plantRootIn-situchemicalSensing). TETRIS consists of screen-printed electrochemical sensors for monitoring concentrations of salt, pH and H2O2 in the root environment of whole plants. TETRIS can detect time-sensitive chemical signals and be operated in parallel through multiplexing to elucidate the overall chemical behavior of living plants. Using TETRIS, we determined the rates of uptake of a range of ions (including nutrients and heavy metals) inBrassica oleracea acephala. We also modulated ion uptake using the ion channel blocker LaCl3, which we could monitor using TETRIS. We developed a machine learning model to predict the rates of uptake of salts, both harmful and beneficial, demonstrating that TETRIS can be used for rapid mapping of ion uptake for new plant varieties. TETRIS has the potential to overcome the urgent "bottleneck" in high-throughput screening in producing high yielding plant varieties with improved resistance against stress.

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Section: Discussionmentioning

confidence: 99%

Section: General Experimental Setup Of Tetrismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Time-Resolved Chemical Phenotyping of Whole Plant Roots with Printed Electrochemical Sensors and Machine Learning

Coatsworth

Cotur

Naik

et al. 2023

Preprint

Self Cite

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“…Temperature and moisture (Biswas et al, 2018) Organic matter and mineral fractions (Biswas et al, 2018) Microbial activities (Biswas et al, 2018); microbiota (Walder et al, 2022); AM fungi (Edlinger et al, 2022) demonstrated that RNAs and miRNAs can be used as marker genes to monitor the onset and transmission of (a)biotic stresses (Loṕez-Galiano et al, 2019;Tyagi et al, 2021;S ̌ecǐćet al, 2021;Zhang et al, 2022;Paes de Melo et al, 2022) species-specific large-scale microarrays which cluster all the (a)biotic stress markers genes available in shoot could be envisaged. Additionally, plant-like miniature adhesive systems for in situ leaf microenvironment monitoring have been recently developed (Fiorello et al, 2021;Coatsworth et al, 2022). The development of plant-inspired miniature machines coupled by sensors to detect in real-time macro-and micronutrients in plant leaves would be instrumental to infer soil nutrient availability and open avenues for their application in precision agrotechnology systems.…”

Section: Stress Resiliencementioning

confidence: 99%

Unearthing soil-plant-microbiota crosstalk: Looking back to move forward

et al. 2023

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The soil is vital for life on Earth and its biodiversity. However, being a non-renewable and threatened resource, preserving soil quality is crucial to maintain a range of ecosystem services critical to ecological balances, food production and human health. In an agricultural context, soil quality is often perceived as the ability to support field production, and thus soil quality and fertility are strictly interconnected. The concept of, as well as the ways to assess, soil fertility has undergone big changes over the years. Crop performance has been historically used as an indicator for soil quality and fertility. Then, analysis of a range of physico-chemical parameters has been used to routinely assess soil quality. Today it is becoming evident that soil quality must be evaluated by combining parameters that refer both to the physico-chemical and the biological levels. However, it can be challenging to find adequate indexes for evaluating soil quality that are both predictive and easy to measure in situ. An ideal soil quality assessment method should be flexible, sensitive enough to detect changes in soil functions, management and climate, and should allow comparability among sites. In this review, we discuss the current status of soil quality indicators and existing databases of harmonized, open-access topsoil data. We also explore the connections between soil biotic and abiotic features and crop performance in an agricultural context. Finally, based on current knowledge and technical advancements, we argue that the use of plant health traits represents a powerful way to assess soil physico-chemical and biological properties. These plant health parameters can serve as proxies for different soil features that characterize soil quality both at the physico-chemical and at the microbiological level, including soil quality, fertility and composition of soil microbial communities.

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“…[ 145 ]. Due to the non-contact nature of measuring light, optical sensors can overcome many problems typically associated with live-plant studies, such as wounding caused during measurements [ 148 ]. Plant surfaces can be profiled by various methods such as Raman spectroscopy, Positron emission tomography, X-ray fluorescence spectroscopy, or spectral imaging that do not require any optical probes [ 149 , 150 , 151 ].…”

Section: Existing Optical Sensing Technologies To Monitor Plant-micro...mentioning

confidence: 99%

Optical Sensing Technologies to Elucidate the Interplay between Plant and Microbes

Neelam

Tabassum

2023

Micromachines

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Plant-microbe interactions are critical for ecosystem functioning and driving rhizosphere processes. To fully understand the communication pathways between plants and rhizosphere microbes, it is crucial to measure the numerous processes that occur in the plant and the rhizosphere. The present review first provides an overview of how plants interact with their surrounding microbial communities, and in turn, are affected by them. Next, different optical biosensing technologies that elucidate the plant-microbe interactions and provide pathogenic detection are summarized. Currently, most of the biosensors used for detecting plant parameters or microbial communities in soil are centered around genetically encoded optical and electrochemical biosensors that are often not suitable for field applications. Such sensors require substantial effort and cost to develop and have their limitations. With a particular focus on the detection of root exudates and phytohormones under biotic and abiotic stress conditions, novel low-cost and in-situ biosensors must become available to plant scientists.

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Continuous monitoring of chemical signals in plants under stress

Cited by 38 publications

References 158 publications

Time-Resolved Chemical Phenotyping of Whole Plant Roots with Printed Electrochemical Sensors and Machine Learning

Time-Resolved Chemical Phenotyping of Whole Plant Roots with Printed Electrochemical Sensors and Machine Learning

Unearthing soil-plant-microbiota crosstalk: Looking back to move forward

Optical Sensing Technologies to Elucidate the Interplay between Plant and Microbes

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