Continuous multicomponent quantification during supercritical fluid extraction applied to microalgae using in-line UV/Vis absorption spectroscopy and on-line evaporative light scattering detection

Abrahamsson, Victor; Jumaah, Firas; Turner, Charlotta

doi:10.1016/j.supflu.2017.09.014

Cited by 21 publications

(10 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, new and more sophisticated equipment, which allow a simultaneous extraction and characterization of compounds, are emerging in the last years. As an example, Abrahamsson et al [111] developed a SFE-UV/Vis-ELSD equipment (Figure 5) capable of detecting carotenoids, chlorophyll A, ergosterol and total lipids from a microalgae extract obtained by SFE. This approach not only simplifies the whole extraction-identification-quantification process but also avoid the possible damage that extracts can suffer during these steps.…”

Section: Extraction Of Bioactive Compounds From Seaweeds and Microalgaementioning

confidence: 99%

Sub- and supercritical fluid extraction of bioactive compounds from plants, food-by-products, seaweeds and microalgae – An update

Gallego

Bueno

Herrero

2019

TrAC Trends in Analytical Chemistry

218

112

View full text Add to dashboard Cite

Following our previous reviews, this manuscript presents an updated perspective on the use of compressed fluids, mainly under sub-and supercritical conditions, for the extraction of bioactive components from natural matrices covering the period from 2015 to present. These extraction technologies might have an important role in the development of sustainable and efficient extraction processes to cope with the high demand of natural bioactive compounds.Moreover, more complex approaches based on process integration, intensification and the development of sequential valorization chains are being increasingly developed. Most recent and interesting applications grouped according to the type of natural material used (plants, seaweeds, microalgae and food-related by-products) are described and critically commented. Furthermore, we discuss the potential future outlooks related to this field in agreement with our own experience.

show abstract

Section: Extraction Of Bioactive Compounds From Seaweeds and Microalgaementioning

confidence: 99%

Sub- and supercritical fluid extraction of bioactive compounds from plants, food-by-products, seaweeds and microalgae – An update

Gallego

Bueno

Herrero

2019

TrAC Trends in Analytical Chemistry

218

112

View full text Add to dashboard Cite

show abstract

“…The on‐line coupling with LC and GC was reviewed by Hyotylainen in 2007 , and more recently by Cifuentes’ research group . Only one manuscript dealing with SFE–GC and SFE–LC has been found in a literature search in Scopus covering the last two years (SFE–UV/Vis–ELSD, ). Regarding SFE–SFC, to the best of our knowledge, eight investigations were conducted in the same period that will be discussed in the present review.…”

Section: On‐line Sfe Coupled To Sfcmentioning

confidence: 99%

Recent applications of on‐line supercritical fluid extraction coupled to advanced analytical techniques for compounds extraction and identification

Sánchez‐Camargo

Parada-Alonso

Ibáñez

2018

J of Separation Science

View full text Add to dashboard Cite

In a previous review (Sánchez-Camargo et al., J. Sep. Sci. 40 (2017) 213-227), we discussed the application of on-line supercritical fluid extraction coupled to chromatographic techniques. This review includes an update of the most recent publications (from January 2016 till June 2018) on this topic, which employs advanced analytical techniques for extracting and identifying valuable analytes. Supercritical fluid extraction has been widely recognized as a green sample preparation technique, because it is efficient, environmentally friendly, powerful, and faster, offering the possibility of direct coupling to analytical instrumental techniques. Among those techniques, supercritical fluid chromatography has experienced an innovative progression in the last 10 years, and the most recent applications of supercritical fluid extraction are coupled to this advanced analytical tool. The general principles, both methodological and instrumental of on-line supercritical fluid extraction coupled to supercritical fluid chromatography are described here. Besides, applications of the mentioned coupling for analysing biological fluids, food, soil, and botanical samples are also presented and discussed. Finally, a brief description about the very recent on-line coupling of supercritical fluid extraction to ion mobility spectrometry is presented, as well as concluding remarks about the importance of using these coupled techniques in the near future.

show abstract

“…CLS model assumes that spectrum of a mixture of multiple components is the sum of spectra of the pure components and requires reference spectra of all pure components [24]. CLS method has been used for separating total carotenoid signal from UV-spectra [25] and for distinguishing between two carotenoid by Raman spectroscopy in human retina [26]. Decomposition of complex Raman spectra into individual pure carotenoids using CLS method has not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Detection of Multiple Carotenoid Pigments in Algae by Raman Spectroscopy and Classical Least Squares (CLS) Analysis

Bhakthavatsalam¹

2020

PSBJ

View full text Add to dashboard Cite

Synthetic carotenoid pigments derived from petrochemicals are used for feed industry. However, due to health safety concerns these synthetic forms cannot be used for direct human consumption. Algae cells naturally produce multiple carotenoid pigments, including astaxanthin and lutein that have antioxidants, antitumor and antiviral properties. Hence, these natural carotenoids have high market value as nutraceutical supplements, and also as natural food coloring and cosmetics ingredient. Cost-efficient extraction and purification of these high value carotenoids from algae is challenging as the pigment profiles of algae cells is complex and dynamic. Cells contain a mixture of multiple pigments dissolved in lipids and the composition varies with growth stages, changes in environment, and nutrient availability. To be commercially beneficial, harvesting and extraction of algal cultures should be done at an optimal time point, when both biomass and the desired high-value carotenoid accumulation are at its peak. Pigment composition and quantity are conventionally determined by chromatography and UV/ IR spectroscopy. These methods although can provide high accuracy level, are dependent on multi-step process of pigment extraction, needs large volume of algal biomass, expensive chemicals, laboratory equipments, and hours of skilled manpower. In this paper, we present a high-throughput method of in situ pigment profiling by Raman spectroscopy. Pigment spectra could be obtained under 5 min from live algae cells, without any extraction, drying or grinding. Identification and relative quantification of 3-6 carotenoid pigments simultaneously from the spectra was possible using CLS analysis method. The method was found to be sensitive, versatile and robust by statistical analysis. This approach can be used for pigment profiling and screening of live algal cell cultures with population variability for real-time assessment of their health and productivity. It additionally has the potential to be used for live pond monitoring using portable Raman spectrometers.

show abstract

Continuous multicomponent quantification during supercritical fluid extraction applied to microalgae using in-line UV/Vis absorption spectroscopy and on-line evaporative light scattering detection

Cited by 21 publications

References 34 publications

Sub- and supercritical fluid extraction of bioactive compounds from plants, food-by-products, seaweeds and microalgae – An update

Sub- and supercritical fluid extraction of bioactive compounds from plants, food-by-products, seaweeds and microalgae – An update

Recent applications of on‐line supercritical fluid extraction coupled to advanced analytical techniques for compounds extraction and identification

Simultaneous Detection of Multiple Carotenoid Pigments in Algae by Raman Spectroscopy and Classical Least Squares (CLS) Analysis

Contact Info

Product

Resources

About