Continuous particle separation based on electrical properties using alternating current dielectrophoresis

Çetin, Barbaros; Li, Dongqing

doi:10.1002/elps.200900078

Cited by 18 publications

(19 citation statements)

References 42 publications

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“…However, it has been shown that this method can predict the particle motion qualitatively. By introducing a scale factor which accounts for the distortion of the electric and flow fields due to the finite particle size, the simulation results can match the experimental results [10,11,45,46,50]. The scaling factor is assumed to be constant and varies between 0 and 1.0 for a given particle size.…”

Section: Modelingmentioning

confidence: 89%

“…In this model, the particle is assumed to be move with a terminal velocity under the action of drag force which is the result of the interaction of the particle with the flow field, and the DEP force which is the result of the interaction of the particle with the electrical field. The details of the modeling of the particle motion can be found in our previous studies [46,50]. Considering the drag force and the DEP force, velocity of the particle can be written as…”

Section: Modelingmentioning

confidence: 99%

“…1. The hurdle geometry is chosen as circular to achieve the most efficient separation [50]. The device has two inlet (A and B) and two exit (C and D) reservoirs.…”

Section: Modelingmentioning

confidence: 99%

“…To simulate the particle trajectories, a model based on Lagrangian tracking method [10,11,45,46,50] is utilized. In this model, the particle is assumed to be move with a terminal velocity under the action of drag force which is the result of the interaction of the particle with the flow field, and the DEP force which is the result of the interaction of the particle with the electrical field.…”

Section: Modelingmentioning

confidence: 99%

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Lab‐on‐a‐chip device for continuous particle and cell separation based on electrical properties via alternating current dielectrophoresis

Çetin

2010

Electrophoresis

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A novel alternating current-dielectrophoresis microfluidic chip was developed to separate particles and cells continuously by their electric properties. The flow is induced by pressure gradient. A pair of simple, 3-D electrodes was used to achieve a localized nonuniform electric field. Dielectrophoretic force is generated in the transverse direction to the flow by inserting the electrodes along the channel side walls. The localized electric field is important to reduce the Joule heating and any adverse effects of electrical field on biological cells. Latex particles of different sizes and white blood cells (8-12 μm) were manipulated successfully and the separation of 10 μm latex particles and white blood cells based on their different electrical properties was demonstrated.

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Section: Modelingmentioning

confidence: 89%

Section: Modelingmentioning

confidence: 99%

“…1. The hurdle geometry is chosen as circular to achieve the most efficient separation [50]. The device has two inlet (A and B) and two exit (C and D) reservoirs.…”

Section: Modelingmentioning

confidence: 99%

Section: Modelingmentioning

confidence: 99%

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Lab‐on‐a‐chip device for continuous particle and cell separation based on electrical properties via alternating current dielectrophoresis

Çetin

2010

Electrophoresis

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“…Inlet velocity was calculated and chosen in simulations as 0.018 m/s to be able to apply 10 μL/min flow rate. Outlet boundary condition was chosen as zero pressure because it is open to atmosphere throughout the tests .…”

Section: Methodsmentioning

confidence: 99%

Label‐free detection of multidrug resistance in K562 cells through isolated 3D‐electrode dielectrophoresis

et al. 2015

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Dielectrophoresis (DEP), a technique used to separate particles based on different sizes and/or dielectric properties under nonuniform electric field, is a promising method to be applied in label-free, rapid, and effective cell manipulation and separation. In this study, a microelectromechanical systems-based, isolated 3D-electrode DEP device has been designed and implemented for the label-free detection of multidrug resistance in K562 leukemia cells, based on the differences in their cytoplasmic conductivities. Cells were hydrodynamically focused to the 3D-electrode arrays, placed on the side walls of the microchannel, through V-shaped parylene-C obstacles. 3D-electrodes extruded along the z-direction provide uniformly distributed DEP force through channel depth. Cell suspension containing resistant and sensitive cancer cells with 1:100 ratio was continuously flown through the channel at a rate of 10 μL/min. Detection was realized at 48.64 MHz, the cross-over frequency of sensitive K562 cells, at which sensitive cells flow with the fluid, while the resistant ones are trapped by positive DEP force. Device can be operated at considerably low voltages (<9 Vpp ). This is achieved by means of a very thin (0.5 μm) parylene coating on electrodes, providing the advantages offered by the isolation of electrodes from the sample, while the working voltage can still be kept low. Results prove that the presented DEP device can provide an efficient platform for the detection of multidrug resistance in leukemia, in a label-free manner.

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Direct current insulator‐based dielectrophoretic characterization of erythrocytes: ABO‐Rh human blood typing

Srivastava¹,

Artemiou²,

Minerick³

2011

Electrophoresis

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A microfluidic platform developed for quantifying the dependence of erythrocyte (red blood cell, RBC) responses by ABO-Rh blood type via direct current insulator dielectrophoresis (DC-iDEP) is presented. The PDMS DC-iDEP device utilized a 400 x 170 μm² rectangular insulating obstacle embedded in a 1.46-cm long, 200-μm wide inlet channel to create spatial non-uniformities in direct current (DC) electric field density realized by separation into four outlet channels. The DC-iDEP flow behaviors were investigated for all eight blood types (A+, A-, B+, B-, AB+, AB-, O+, O-) in the human ABO-Rh blood typing system. Three independent donors of each blood type, same donor reproducibility, different conductivity buffers (0.52-9.1 mS/cm), and DC electric fields (17.1-68.5 V/cm) were tested to investigate separation dependencies. The data analysis was conducted from image intensity profiles across inlet and outlet channels in the device. Individual channel fractions suggest that the dielectrophoretic force experienced by the cells is dependent on erythrocyte antigen expression. Two different statistical analysis methods were conducted to determine how distinguishable a single blood type was from the others. Results indicate that channel fraction distributions differ by ABO-Rh blood types suggesting that antigens present on the erythrocyte membrane polarize differently in DC-iDEP fields. Under optimized conductivity and field conditions, certain blind blood samples could be sorted with low misclassification rates.

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Continuous particle separation based on electrical properties using alternating current dielectrophoresis

Cited by 18 publications

References 42 publications

Lab‐on‐a‐chip device for continuous particle and cell separation based on electrical properties via alternating current dielectrophoresis

Lab‐on‐a‐chip device for continuous particle and cell separation based on electrical properties via alternating current dielectrophoresis

Label‐free detection of multidrug resistance in K562 cells through isolated 3D‐electrode dielectrophoresis

Direct current insulator‐based dielectrophoretic characterization of erythrocytes: ABO‐Rh human blood typing

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